Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication

Detalhes bibliográficos
Autor(a) principal: Real-Hohn, Antonio
Data de Publicação: 2017
Outros Autores: Provance, D. William, Gonçalves, Rafael Braga, Denani, Caio Bidueira, Oliveira, Andrea Cheble de, Salerno, Verônica P., Gomes, Andre Marco Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59574
Resumo: Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Universidade Federal Rio do Janeiro. Centro de Ciências da Saúde. Escola de Educação Física e Desportos. Departamento de Biociências da Atividade Física. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.
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spelling Real-Hohn, AntonioProvance, D. WilliamGonçalves, Rafael BragaDenani, Caio BidueiraOliveira, Andrea Cheble deSalerno, Verônica P.Gomes, Andre Marco Oliveira2023-07-13T19:29:35Z2023-07-13T19:29:35Z2017REAL-HOHN, Antonio et al. Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication. Scientific Reports, v. 7, n. 1, p. 1-15, Dec. 2017.2045-2322https://www.arca.fiocruz.br/handle/icict/5957410.1038/S41598-017-17501-Z2045-2322engNature Publishing GroupActin CytoskeletonEnterovirus InfectionsHeLa CellsHumansMethylmalonic AcidMyosin Heavy ChainsMyosin Type VMyosin-Light-Chain KinasePhosphorylationRhinovirusVirus InternalizationVirus ReplicationRecycling endosomesHela-cellsIn-vivoRNAVirusEntryEndocytosisExpressionInfectionMultipleImpairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replicationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Universidade Federal Rio do Janeiro. Centro de Ciências da Saúde. Escola de Educação Física e Desportos. Departamento de Biociências da Atividade Física. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Instituto Biomédico. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brazil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Universidade Federal Rio do Janeiro. Centro de Ciências da Saúde. Escola de Educação Física e Desportos. Departamento de Biociências da Atividade Física. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brazil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Together, the three human rhinovirus (RV) species are the most frequent cause of the common cold. Because of their high similarity with other viral species of the genus Enterovirus, within the large family Picornaviridae, studies on RV infectious activities often offer a less pathogenic model for more aggressive enteroviruses, e.g. poliovirus or EV71. Picornaviruses enter via receptor mediated endocytosis and replicate in the cytosol. Most of them depend on functional F-actin, Rab proteins, and probably motor proteins. To assess the latter, we evaluated the role of myosin light chain kinase (MLCK) and two myosin V isoforms (Va and Vb) in RV-B14 infection. We report that ML-9, a very specific MLCK inhibitor, dramatically reduced RV-B14 entry. We also demonstrate that RV-B14 infection in cells expressing dominant-negative forms of myosin Va and Vb was impaired after virus entry. Using immunofluorescent localization and immunoprecipitation, we show that myosin Va co-localized with RV-B14 exclusively after viral entry (15 min post infection) and that myosin Vb was present in the clusters of newly synthesized RNA in infected cells. These clusters, observed at 180 min post infection, are reminiscent of replication sites. Taken together, these results identify myosin light chain kinase, myosin Va and myosin Vb as new players in RV-B14 infection that participate directly or indirectly in different stages of the viral cycle.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59574/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL10.1038_s41598-017-17501-z.pdfapplication/pdf3957442https://www.arca.fiocruz.br/bitstream/icict/59574/2/10.1038_s41598-017-17501-z.pdfb228c4166a8b440a90ea47f1bafe222dMD52icict/595742023-07-20 15:06:18.206oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-20T18:06:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
title Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
spellingShingle Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
Real-Hohn, Antonio
Actin Cytoskeleton
Enterovirus Infections
HeLa Cells
Humans
Methylmalonic Acid
Myosin Heavy Chains
Myosin Type V
Myosin-Light-Chain Kinase
Phosphorylation
Rhinovirus
Virus Internalization
Virus Replication
Recycling endosomes
Hela-cells
In-vivo
RNA
Virus
Entry
Endocytosis
Expression
Infection
Multiple
title_short Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
title_full Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
title_fullStr Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
title_full_unstemmed Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
title_sort Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
author Real-Hohn, Antonio
author_facet Real-Hohn, Antonio
Provance, D. William
Gonçalves, Rafael Braga
Denani, Caio Bidueira
Oliveira, Andrea Cheble de
Salerno, Verônica P.
Gomes, Andre Marco Oliveira
author_role author
author2 Provance, D. William
Gonçalves, Rafael Braga
Denani, Caio Bidueira
Oliveira, Andrea Cheble de
Salerno, Verônica P.
Gomes, Andre Marco Oliveira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Real-Hohn, Antonio
Provance, D. William
Gonçalves, Rafael Braga
Denani, Caio Bidueira
Oliveira, Andrea Cheble de
Salerno, Verônica P.
Gomes, Andre Marco Oliveira
dc.subject.mesh.en_US.fl_str_mv Actin Cytoskeleton
Enterovirus Infections
HeLa Cells
Humans
Methylmalonic Acid
Myosin Heavy Chains
Myosin Type V
Myosin-Light-Chain Kinase
Phosphorylation
Rhinovirus
Virus Internalization
Virus Replication
topic Actin Cytoskeleton
Enterovirus Infections
HeLa Cells
Humans
Methylmalonic Acid
Myosin Heavy Chains
Myosin Type V
Myosin-Light-Chain Kinase
Phosphorylation
Rhinovirus
Virus Internalization
Virus Replication
Recycling endosomes
Hela-cells
In-vivo
RNA
Virus
Entry
Endocytosis
Expression
Infection
Multiple
dc.subject.en.en_US.fl_str_mv Recycling endosomes
Hela-cells
In-vivo
RNA
Virus
Entry
Endocytosis
Expression
Infection
Multiple
description Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Universidade Federal Rio do Janeiro. Centro de Ciências da Saúde. Escola de Educação Física e Desportos. Departamento de Biociências da Atividade Física. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2023-07-13T19:29:35Z
dc.date.available.fl_str_mv 2023-07-13T19:29:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REAL-HOHN, Antonio et al. Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication. Scientific Reports, v. 7, n. 1, p. 1-15, Dec. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59574
dc.identifier.issn.en_US.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/S41598-017-17501-Z
dc.identifier.eissn.none.fl_str_mv 2045-2322
identifier_str_mv REAL-HOHN, Antonio et al. Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication. Scientific Reports, v. 7, n. 1, p. 1-15, Dec. 2017.
2045-2322
10.1038/S41598-017-17501-Z
url https://www.arca.fiocruz.br/handle/icict/59574
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59574/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/59574/2/10.1038_s41598-017-17501-z.pdf
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