Endothelial cell invasion is controlled by dactylopodia

Detalhes bibliográficos
Autor(a) principal: Figueiredo, Ana
Data de Publicação: 2021
Outros Autores: Barbacena, Pedro, Russo, Ana, Vaccaro, Silvia, Ramalho, Daniela, Pena, Andreia, Lima, Aida Pires, Rua Ferreira, Rita, Fidalgo, Marta, El-Marjou, Fatima, Carvalho, Yulia, Vasconcelos, Francisca, Lennon-Duménil, Ana-Maria, Vignjevic, Danijela Matic, Franco, Claudio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/47603
Resumo: Copyright © 2021 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
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spelling Endothelial cell invasion is controlled by dactylopodiaAngiogenesisEndothelial cellsInvasionActinMyosinCopyright © 2021 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).Sprouting angiogenesis is fundamental for development and contributes to cancer, diabetic retinopathy, and cardiovascular diseases. Sprouting angiogenesis depends on the invasive properties of endothelial tip cells. However, there is very limited knowledge on how tip cells invade into tissues. Here, we show that endothelial tip cells use dactylopodia as the main cellular protrusion for invasion into nonvascular extracellular matrix. We show that dactylopodia and filopodia protrusions are balanced by myosin IIA (NMIIA) and actin-related protein 2/3 (Arp2/3) activity. Endothelial cell-autonomous ablation of NMIIA promotes excessive dactylopodia formation in detriment of filopodia. Conversely, endothelial cell-autonomous ablation of Arp2/3 prevents dactylopodia development and leads to excessive filopodia formation. We further show that NMIIA inhibits Rac1-dependent activation of Arp2/3 by regulating the maturation state of focal adhesions. Our discoveries establish a comprehensive model of how endothelial tip cells regulate its protrusive activity and will pave the way toward strategies to block invasive tip cells during sprouting angiogenesis.C.A.F. was supported by a European Research Council starting grant (679368), the European Union H2020-TWINN-2015—Twinning (692322), the Fundação para a Ciência e a Tecnologia funding (grants IF/00412/2012, EXPL/BEX-BCM/2258/2013, PTDC/MED-PAT/31639/2017, and PTDC/BIA-CEL/32180/2017 and fellowships CEECIND/04251/2017 and CEECIND/02589/2018), and a grant from the Fondation Leducq (17CVD03).National Academy of SciencesRepositório da Universidade de LisboaFigueiredo, AnaBarbacena, PedroRusso, AnaVaccaro, SilviaRamalho, DanielaPena, AndreiaLima, Aida PiresRua Ferreira, RitaFidalgo, MartaEl-Marjou, FatimaCarvalho, YuliaVasconcelos, FranciscaLennon-Duménil, Ana-MariaVignjevic, Danijela MaticFranco, Claudio2021-04-29T13:11:19Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/47603engProc Natl Acad Sci U S A. 2021 May 4;118(18):e20238291180027-842410.1073/pnas.20238291181091-6490info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:50:36Zoai:repositorio.ul.pt:10451/47603Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:59:36.391389Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Endothelial cell invasion is controlled by dactylopodia
title Endothelial cell invasion is controlled by dactylopodia
spellingShingle Endothelial cell invasion is controlled by dactylopodia
Figueiredo, Ana
Angiogenesis
Endothelial cells
Invasion
Actin
Myosin
title_short Endothelial cell invasion is controlled by dactylopodia
title_full Endothelial cell invasion is controlled by dactylopodia
title_fullStr Endothelial cell invasion is controlled by dactylopodia
title_full_unstemmed Endothelial cell invasion is controlled by dactylopodia
title_sort Endothelial cell invasion is controlled by dactylopodia
author Figueiredo, Ana
author_facet Figueiredo, Ana
Barbacena, Pedro
Russo, Ana
Vaccaro, Silvia
Ramalho, Daniela
Pena, Andreia
Lima, Aida Pires
Rua Ferreira, Rita
Fidalgo, Marta
El-Marjou, Fatima
Carvalho, Yulia
Vasconcelos, Francisca
Lennon-Duménil, Ana-Maria
Vignjevic, Danijela Matic
Franco, Claudio
author_role author
author2 Barbacena, Pedro
Russo, Ana
Vaccaro, Silvia
Ramalho, Daniela
Pena, Andreia
Lima, Aida Pires
Rua Ferreira, Rita
Fidalgo, Marta
El-Marjou, Fatima
Carvalho, Yulia
Vasconcelos, Francisca
Lennon-Duménil, Ana-Maria
Vignjevic, Danijela Matic
Franco, Claudio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Figueiredo, Ana
Barbacena, Pedro
Russo, Ana
Vaccaro, Silvia
Ramalho, Daniela
Pena, Andreia
Lima, Aida Pires
Rua Ferreira, Rita
Fidalgo, Marta
El-Marjou, Fatima
Carvalho, Yulia
Vasconcelos, Francisca
Lennon-Duménil, Ana-Maria
Vignjevic, Danijela Matic
Franco, Claudio
dc.subject.por.fl_str_mv Angiogenesis
Endothelial cells
Invasion
Actin
Myosin
topic Angiogenesis
Endothelial cells
Invasion
Actin
Myosin
description Copyright © 2021 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
publishDate 2021
dc.date.none.fl_str_mv 2021-04-29T13:11:19Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/47603
url http://hdl.handle.net/10451/47603
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Proc Natl Acad Sci U S A. 2021 May 4;118(18):e2023829118
0027-8424
10.1073/pnas.2023829118
1091-6490
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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