Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases

Detalhes bibliográficos
Autor(a) principal: Rabello, Vanessa Brito Souza
Data de Publicação: 2022
Outros Autores: Almeida-Silva, Fernando, Scramignon-Costa, Bruno de Souza, Motta, Beatriz da Silva, Macedo, Priscila Marques de, Teixeira, Marcus de Melo, Almeida-Paes, Rodrigo, Irinyi, Laszlo, Meyer, Wieland, Zancopé-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/55330
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Rabello, Vanessa Brito SouzaAlmeida-Silva, FernandoScramignon-Costa, Bruno de SouzaMotta, Beatriz da SilvaMacedo, Priscila Marques deTeixeira, Marcus de MeloAlmeida-Paes, RodrigoIrinyi, LaszloMeyer, WielandZancopé-Oliveira, Rosely Maria2022-10-25T18:06:32Z2022-10-25T18:06:32Z2022RABELLO, Vanessa Brito Souza et al. Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases. Frontiers in cellular and infection microbiology, v. 12, p. 1-7, 2022.2235-2988https://www.arca.fiocruz.br/handle/icict/5533010.3389/fcimb.2022.894297engFrontiers MediaEnvironmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Casesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Universidade de Brasília. Faculdade de Medicina. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Marie Bashir Institute for Infectious Diseases and Biosecurity. Westmead Clinical School. Sydney Medical School. Faculty of Medicine and Health. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / University of Sydney. Westmead Hospital-Research and Education Network. Westmead Institute for Medical Research. Sydney, NSW, Australia.Marie Bashir Institute for Infectious Diseases and Biosecurity. Westmead Clinical School. Sydney Medical School. Faculty of Medicine and Health. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia. / University of Sydney. Westmead Hospital-Research and Education Network. Westmead Institute for Medical Research. Sydney, NSW, Australia / Curtin University. Curtin Medical School. Perth, WA, Australia.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Sporotrichosis has been expanding throughout the Brazilian territory in recent years. New outbreaks have emerged, and consequently, the sporotrichosis agents, mainly Sporothrix brasiliensis, should remain in the environment somehow. Therefore, the aim of this study was to investigate the presence of Sporothrix spp. in the environment from an area of ​​the Rio de Janeiro state, Brazil, with recurrent cases of human and animal sporotrichosis. Abandoned demolition timber wood samples were collected in the garden of a house where the cases of human and feline sporotrichosis have occurred in the last 10 years. The environmental survey revealed a Sporothrix spp. colony from the serial dilution cultures of one abandoned demolition wood sample. In addition, a fungal strain isolated from a cat with skin lesions that lived in the house was also included in the study. The species-specific PCR, and calmodulin partial sequencing identified the environmental and cat isolates as S. brasiliensis. Furthermore, the phylogenetic analysis performed with the partial sequences of internal transcribed spacer region and constitutive genes (calmodulin, β-tubulin, and chitin synthase) showed high similarity between environmental and cat isolates from the same geographic region. Moreover, the antifungal susceptibility test revealed that the minimal inhibitory concentration of itraconazole from the environment isolate was lower than the cat isolate, while amphotericin B and terbinafine were similar. Our results show that S. brasiliensis is able to maintain itself in the environmental material for years. With this, we corroborate that the eco-epidemiology of sporotrichosis is not well understood, and despite the major occurrence of S. brasiliensis in Brazil, it is rarely isolated from the environment.BrazilSporothrix brasiliensisEnvironmentPhylogenetic analysisSporotrichosisTimber woodinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55330/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEnvironmental_Vanessa_Rabello_etal_INI_2022.pdfEnvironmental_Vanessa_Rabello_etal_INI_2022.pdfapplication/pdf1383553https://www.arca.fiocruz.br/bitstream/icict/55330/2/Environmental_Vanessa_Rabello_etal_INI_2022.pdf4b207bd6f62590b2f5395a5b70c78c79MD52icict/553302022-10-25 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dc.title.en_US.fl_str_mv Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
spellingShingle Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
Rabello, Vanessa Brito Souza
Brazil
Sporothrix brasiliensis
Environment
Phylogenetic analysis
Sporotrichosis
Timber wood
title_short Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_full Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_fullStr Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_full_unstemmed Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
title_sort Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases
author Rabello, Vanessa Brito Souza
author_facet Rabello, Vanessa Brito Souza
Almeida-Silva, Fernando
Scramignon-Costa, Bruno de Souza
Motta, Beatriz da Silva
Macedo, Priscila Marques de
Teixeira, Marcus de Melo
Almeida-Paes, Rodrigo
Irinyi, Laszlo
Meyer, Wieland
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Almeida-Silva, Fernando
Scramignon-Costa, Bruno de Souza
Motta, Beatriz da Silva
Macedo, Priscila Marques de
Teixeira, Marcus de Melo
Almeida-Paes, Rodrigo
Irinyi, Laszlo
Meyer, Wieland
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rabello, Vanessa Brito Souza
Almeida-Silva, Fernando
Scramignon-Costa, Bruno de Souza
Motta, Beatriz da Silva
Macedo, Priscila Marques de
Teixeira, Marcus de Melo
Almeida-Paes, Rodrigo
Irinyi, Laszlo
Meyer, Wieland
Zancopé-Oliveira, Rosely Maria
dc.subject.en.en_US.fl_str_mv Brazil
Sporothrix brasiliensis
Environment
Phylogenetic analysis
Sporotrichosis
Timber wood
topic Brazil
Sporothrix brasiliensis
Environment
Phylogenetic analysis
Sporotrichosis
Timber wood
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-10-25T18:06:32Z
dc.date.available.fl_str_mv 2022-10-25T18:06:32Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RABELLO, Vanessa Brito Souza et al. Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases. Frontiers in cellular and infection microbiology, v. 12, p. 1-7, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/55330
dc.identifier.issn.en_US.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2022.894297
identifier_str_mv RABELLO, Vanessa Brito Souza et al. Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases. Frontiers in cellular and infection microbiology, v. 12, p. 1-7, 2022.
2235-2988
10.3389/fcimb.2022.894297
url https://www.arca.fiocruz.br/handle/icict/55330
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/55330/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/55330/2/Environmental_Vanessa_Rabello_etal_INI_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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