Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57635 |
Resumo: | Department of Virology. Parasitology and Immunology. Ghent University. Merelbeke, Belgium. |
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Cools, PietVlaminck, JohnnyAlbonico, MarcoAme, ShaaliAyana, MioAntonio, Barrios Perez JoséCringoli, GiuseppeDana, DanielKeiser, JenniferMaurelli, Maria PMaya, CatalinaMatoso, Leonardo FMontresor, AntonioMekonnen, ZelekeMirams, GregOliveira, Rodrigo Corrêa dePinto, Simone ARinaldi, LauraSayasone, SomphouThomas, EurionVerweij, Jaco JVercruysse, JozefLevecke, Bruno2023-03-31T13:17:21Z2023-03-31T13:17:21Z2019COOLS, Piet et al. Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. PLoS Negl Trop Dis., v. 13, n. 8, e0007446, 2019. doi: 10.1371/journal.pntd.0007446.1935-2735https://www.arca.fiocruz.br/handle/icict/5763510.1371/journal.pntd.0007446engPublic Library of ScienceDiagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countriesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Virology. Parasitology and Immunology. Ghent University. Merelbeke, Belgium.Department of Virology. Parasitology and Immunology. Ghent University. Merelbeke, Belgium.Center for Tropical Diseases. Sacro Cuore Don Calabria Hospital. Negrar, Italy/Department of Life Sciences and Systems Biology. University of Turin. Italy.Public Health Laboratory-Ivo de Carneri. Chake Chake. United Republic of Tanzania.Jimma University Institute of Health. Jimma University. Jimma, Ethiopia.Engineering Institute of National Autonomous University of Mexico. Mexico City, Mexico.Department of Veterinary Medicine and Animal Production. University of Naples Federico II. Naples, Italy.Jimma University Institute of Health. Jimma University. Jimma, Ethiopia.Department of Medical Parasitology and Infection Biology. Swiss Tropical and Public Health Institute. Basel, Switzerland/University of Basel. Basel, Switzerland.Department of Veterinary Medicine and Animal Production. University of Naples Federico II. Naples, Italy.Engineering Institute of National Autonomous University of Mexico. Mexico City, Mexico.Laboratory of Molecular and Cellular Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Department of Control of Neglected Tropical Diseases. World Health Organization. Geneva, Switzerland.Jimma University Institute of Health. Jimma University. Jimma, Ethiopia.Techion Group Ltd. Dunedin, New Zealand.Laboratory of Molecular and Cellular Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Laboratory of Molecular and Cellular Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Department of Veterinary Medicine and Animal Production. University of Naples Federico II. Naples, Italy.Lao Tropical and Public Health Institute. Ministry of Health. Vientiane, Lao People's Democratic Republic.Techion Group Ltd. Aberystwyth, United Kingdom.Laboratory for Medical Microbiology and Immunology. Elisabeth-Tweesteden Hospital. Tilburg, The Netherlands.Department of Virology. Parasitology and Immunology. Ghent University. Merelbeke, Belgium.Department of Virology. Parasitology and Immunology. Ghent University. Merelbeke, Belgium.Background: Because the success of deworming programs targeting soil-transmitted helminths (STHs) is evaluated through the periodically assessment of prevalence and infection intensities, the use of the correct diagnostic method is of utmost importance. The STH community has recently published for each phase of a deworming program the minimal criteria that a potential diagnostic method needs to meet, the so-called target product profiles (TPPs). Methodology: We compared the diagnostic performance of a single Kato-Katz (reference method) with that of other microscopy-based methods (duplicate Kato-Katz, Mini-FLOTAC and FECPAK(G2)) and one DNA-based method (qPCR) for the detection and quantification of STH infections in three drug efficacy trials in Ethiopia, Lao PDR, and Tanzania. Furthermore, we evaluated a selection of minimal diagnostic criteria of the TPPs. Principal findings: All diagnostic methods showed a clinical sensitivity of >= 90% for all STH infections of moderate-to-heavy intensities. For infections of very low intensity, only qPCR resulted in a sensitivity that was superior to a single Kato-Katz for all STHs. Compared to the reference method, both Mini-FLOTAC and FECPAK(G2) resulted in significantly lower fecal egg counts for some STHs, leading to a substantial underestimation of the infection intensity. For qPCR, there was a positive significant correlation between the egg counts of a single Kato-Katz and the DNA concentration. Conclusions/Significance: Our results indicate that the diagnostic performance of a single Kato-Katz is underestimated by the community and that diagnostic specific thresholds to classify intensity of infection are warranted for Mini-FLOTAC, FECPAK(G2) and qPCR. When we strictly apply the TPPs, Kato-Katz is the only microscopy-based method that meets the minimal diagnostic criteria for application in the planning, monitoring and evaluation phase of an STH program. qPCR is the only method that could be considered in the phase that aims to seek confirmation for cessation of program.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDiagnostic performance of a single and duplicate.pdfDiagnostic performance of a single and duplicate.pdfapplication/pdf2357605https://www.arca.fiocruz.br/bitstream/icict/57635/2/Diagnostic%20performance%20of%20a%20single%20and%20duplicate.pdffa89b8591ee9ee6efd16229a838928a6MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/57635/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/576352023-03-31 10:17:22.238oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-31T13:17:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries |
| title |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries |
| spellingShingle |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries Cools, Piet |
| title_short |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries |
| title_full |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries |
| title_fullStr |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries |
| title_full_unstemmed |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries |
| title_sort |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries |
| author |
Cools, Piet |
| author_facet |
Cools, Piet Vlaminck, Johnny Albonico, Marco Ame, Shaali Ayana, Mio Antonio, Barrios Perez José Cringoli, Giuseppe Dana, Daniel Keiser, Jennifer Maurelli, Maria P Maya, Catalina Matoso, Leonardo F Montresor, Antonio Mekonnen, Zeleke Mirams, Greg Oliveira, Rodrigo Corrêa de Pinto, Simone A Rinaldi, Laura Sayasone, Somphou Thomas, Eurion Verweij, Jaco J Vercruysse, Jozef Levecke, Bruno |
| author_role |
author |
| author2 |
Vlaminck, Johnny Albonico, Marco Ame, Shaali Ayana, Mio Antonio, Barrios Perez José Cringoli, Giuseppe Dana, Daniel Keiser, Jennifer Maurelli, Maria P Maya, Catalina Matoso, Leonardo F Montresor, Antonio Mekonnen, Zeleke Mirams, Greg Oliveira, Rodrigo Corrêa de Pinto, Simone A Rinaldi, Laura Sayasone, Somphou Thomas, Eurion Verweij, Jaco J Vercruysse, Jozef Levecke, Bruno |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cools, Piet Vlaminck, Johnny Albonico, Marco Ame, Shaali Ayana, Mio Antonio, Barrios Perez José Cringoli, Giuseppe Dana, Daniel Keiser, Jennifer Maurelli, Maria P Maya, Catalina Matoso, Leonardo F Montresor, Antonio Mekonnen, Zeleke Mirams, Greg Oliveira, Rodrigo Corrêa de Pinto, Simone A Rinaldi, Laura Sayasone, Somphou Thomas, Eurion Verweij, Jaco J Vercruysse, Jozef Levecke, Bruno |
| description |
Department of Virology. Parasitology and Immunology. Ghent University. Merelbeke, Belgium. |
| publishDate |
2019 |
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2019 |
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2023-03-31T13:17:21Z |
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2023-03-31T13:17:21Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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COOLS, Piet et al. Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. PLoS Negl Trop Dis., v. 13, n. 8, e0007446, 2019. doi: 10.1371/journal.pntd.0007446. |
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https://www.arca.fiocruz.br/handle/icict/57635 |
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1935-2735 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0007446 |
| identifier_str_mv |
COOLS, Piet et al. Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. PLoS Negl Trop Dis., v. 13, n. 8, e0007446, 2019. doi: 10.1371/journal.pntd.0007446. 1935-2735 10.1371/journal.pntd.0007446 |
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https://www.arca.fiocruz.br/handle/icict/57635 |
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eng |
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eng |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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