Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages

Detalhes bibliográficos
Autor(a) principal: Niehus, Sebastian
Data de Publicação: 2014
Outros Autores: Smith, Terry K., Azzouz, Nahid, Campos, Marco Antônio Silva, Dubremetz, Jean François, Gazzinelli, Ricardo Tostes, Schwarz, Ralph T., Grockiego, Françoise Debierre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9423
Resumo: Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany /Institute of Molecular and Cellular Biology. Strasbourg, France.
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spelling Niehus, SebastianSmith, Terry K.Azzouz, NahidCampos, Marco Antônio SilvaDubremetz, Jean FrançoisGazzinelli, Ricardo TostesSchwarz, Ralph T.Grockiego, Françoise Debierre2015-02-04T11:32:14Z2015-02-04T11:32:14Z2014NIEHUS, Sebastian et al. Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages. PLoS One, v. 9, n. 1, p. 1-11, 2014.1553-7366https://www.arca.fiocruz.br/handle/icict/942310.1371/journal.pone.0085386. eCollection 2014engPublic Library of ScienceVirulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePhilipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany /Institute of Molecular and Cellular Biology. Strasbourg, France.University of St Andrews. Biomedical Sciences Research Complex. St Andrews, United Kingdom.Philipps University. Institute for Virology.Laboratory of Parasitology. Marburg, Germany.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil.University of Montpellier. Montpellier, France.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Departamento de Bioquimica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG,Brazil / University of Massachusetts Medical School. Department of Medicine. Division of Infectious Diseases and Immunology. Worcester, MA, USA.Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany/Lille 1 University. Unit of Structural and Functional Glycobiology. Lille, France.Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany/ University of Tours. Infectiology and Public Health. Tours, France.Glycosylphosphatidylinositols (GPIs) from several protozoan parasites are thought to elicit a detrimental stimulation of the host innate immune system aside their main function to anchor surface proteins. Here we analyzed the GPI biosynthesis of an avirulent Toxoplasma gondii type 2 strain (PTG) by metabolic radioactive labeling. We determined the biological function of individual GPI species in the PTG strain in comparison with previously characterized GPI-anchors of a virulent strain (RH). The GPI intermediates of both strains were structurally similar, however the abundance of two of six GPI intermediates was significantly reduced in the PTG strain. The side-by-side comparison of GPI-anchor content revealed that the PTG strain had only ∼ 34% of the protein-free GPIs as well as ∼ 70% of the GPI-anchored proteins with significantly lower rates of protein N-glycosylation compared to the RH strain. All mature GPIs from both strains induced comparable secretion levels of TNF-α and IL-12p40, and initiated TLR4/MyD88-dependent NF-κBp65 activation in macrophages. Taken together, these results demonstrate that PTG and RH strains differ in their GPI biosynthesis and possess significantly different GPI-anchor content, while individual GPI species of both strains induce similar biological functions in macrophages.Toxoplasma gondiiCercopithecus aethiopsGlycosylphosphatidylinositols/metabolismToxoplasma/pathogenicityTumor Necrosis Factor-alpha/metabolisminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9423/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_032.pdf2014_032.pdfapplication/pdf1758820https://www.arca.fiocruz.br/bitstream/icict/9423/2/2014_032.pdf6564483393e41127e01d66919a01df72MD52TEXT2014_032.pdf.txt2014_032.pdf.txtExtracted texttext/plain59375https://www.arca.fiocruz.br/bitstream/icict/9423/3/2014_032.pdf.txt7010034ab780110154a3041a25ff295cMD53icict/94232019-06-19 10:12:13.499oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:12:13Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
title Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
spellingShingle Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
Niehus, Sebastian
Toxoplasma gondii
Cercopithecus aethiops
Glycosylphosphatidylinositols/metabolism
Toxoplasma/pathogenicity
Tumor Necrosis Factor-alpha/metabolism
title_short Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
title_full Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
title_fullStr Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
title_full_unstemmed Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
title_sort Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages
author Niehus, Sebastian
author_facet Niehus, Sebastian
Smith, Terry K.
Azzouz, Nahid
Campos, Marco Antônio Silva
Dubremetz, Jean François
Gazzinelli, Ricardo Tostes
Schwarz, Ralph T.
Grockiego, Françoise Debierre
author_role author
author2 Smith, Terry K.
Azzouz, Nahid
Campos, Marco Antônio Silva
Dubremetz, Jean François
Gazzinelli, Ricardo Tostes
Schwarz, Ralph T.
Grockiego, Françoise Debierre
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Niehus, Sebastian
Smith, Terry K.
Azzouz, Nahid
Campos, Marco Antônio Silva
Dubremetz, Jean François
Gazzinelli, Ricardo Tostes
Schwarz, Ralph T.
Grockiego, Françoise Debierre
dc.subject.en.pt_BR.fl_str_mv Toxoplasma gondii
Cercopithecus aethiops
Glycosylphosphatidylinositols/metabolism
Toxoplasma/pathogenicity
Tumor Necrosis Factor-alpha/metabolism
topic Toxoplasma gondii
Cercopithecus aethiops
Glycosylphosphatidylinositols/metabolism
Toxoplasma/pathogenicity
Tumor Necrosis Factor-alpha/metabolism
description Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany /Institute of Molecular and Cellular Biology. Strasbourg, France.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-02-04T11:32:14Z
dc.date.available.fl_str_mv 2015-02-04T11:32:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv NIEHUS, Sebastian et al. Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages. PLoS One, v. 9, n. 1, p. 1-11, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9423
dc.identifier.issn.none.fl_str_mv 1553-7366
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0085386. eCollection 2014
identifier_str_mv NIEHUS, Sebastian et al. Virulent and avirulent strains of Toxoplasma gondii which differ in their glycosylphosphatidylinositol content induce similar biological functions in macrophages. PLoS One, v. 9, n. 1, p. 1-11, 2014.
1553-7366
10.1371/journal.pone.0085386. eCollection 2014
url https://www.arca.fiocruz.br/handle/icict/9423
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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