Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic
| Autor(a) principal: | |
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| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/34560 |
Resumo: | O artigo encontra-se disponível em acesso aberto no site do Editor. |
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Daltro, Ramona TavaresLeony, Leonardo MaiaFreitas, Natália Erdens MaronSilva, Ângelo Antônio OliveiraSantos, Emily FerreiraDel-Rei, Rodrigo PimentaBrito, Maria Edileuza FelintoBrandão Filho, Sinval PintoGomes, Yara MirandaSilva, Marcelo SousaDonato, Silvia TavaresJeronimo, Selma Maria BezerraMonteiro, Gloria Regina de GóisCarvalho, Lucas PedreiraMagalhães, Andréa SantosZanchin, Nilson Ivo ToninCeledon, Paola Alejandra FioraniSantos, Fred Luciano Neves2019-08-01T14:02:44Z2019-08-01T14:02:44Z2019DALTRO, Ramona Tavares et al. Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic. Journal of Clinical Microbiology, v. 57, n. 8, p. 1-10, 2019.0095-1137https://www.arca.fiocruz.br/handle/icict/3456010.1128/JCM.00762-19O artigo encontra-se disponível em acesso aberto no site do Editor.FIOCRUZ Technical Platform program for providing antigen purification infrastructure. Internal Research Support Program–PIAP Jovem (grant PIAP-001/2017); the Coordination of Superior Level Staff Improvement–CAPES (grant PROEX 0720/2018), the National Council for Scientific and Technological Development– CNPq (grants 479046/2011-5 and 400331/2012-8), Araucária Foundation (grant 05/2016), and Research Support Foundation of the State of Bahia (FAPESB).2020-01-06Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Faculty of Technology and Sciences of Bahia. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Federal University of Rio Grande do Norte. Natal, RN, Brazil / Institute of Hygiene and Tropical Medicine. Lisbon, Portugal.Federal University of Rio Grande do Norte. Natal, RN, Brazil.Federal University of Rio Grande do Norte. Natal, RN, Brazil.Federal University of Rio Grande do Norte. Natal, RN, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil.Federal University of Bahia. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Molecular Biology Institute of Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Chimeric T. cruzi antigens have been proposed as a diagnostic tool for chronic Chagas disease (CD) in both settings where Chagas disease is endemic and those where it is not endemic. Antibody response varies in accordance to each T. cruzi strain, presenting challenges to the use of antigens lacking demonstrated cross-reactivity with Leishmania spp. Our group expressed four chimeric proteins (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) and previously assessed their diagnostic performance to determine cross-reactivity with Leishmania spp. Here, we validated our findings using serum samples from different Brazilian geographic areas reporting endemic Chagas disease, endemic visceral or American cutaneous leishmaniasis (ACL), or both. Overall, 829 serum samples were evaluated using commercial and IBMP enzyme-linked immunosorbent assays. Due to the absence of a reference assay to diagnosis CD, latent class analysis (LCA) was performed through the use of a statistical model. The incidence of cross-reactivity for ACL-positive samples varied from 0.35% (IBMP-8.3) to 0.70% (IBMP-8.1 and IBMP-8.2). Regarding visceral leishmaniasis (VL)-positive samples, the IBMP-8.2 and IBMP-8.3 antigens cross-reacted with six (3.49%) and with only one sample (0.58%), respectively. No cross-reactivity with either ACL or VL was observed for the IBMP-8.4 antigen. Similarly, no cross-reactions were found when VL-positive samples were assayed with IBMP-8.1. The agreement among the results obtained using IBMP antigens ranged from 97.3% for IBMP-8.2 and 99% for IBMP-8.1 and IBMP-8.3 to 100% for IBMP-8.4, demonstrating almost perfect agreement with LCA. Accordingly, in light of the negligible cross-reactivity with both ACL and VL, we suggest the use of IBMP antigens in regions where T. cruzi and Leishmania spp. are coendemic.engAmerican Society for MicrobiologyLeishmaniose tegumentar americanaDoença de ChagasReatividade cruzadaAntígenos quiméricos recombinantesLeishmaniose visceralAmerican cutaneous leishmaniasisChagas diseaseCross-reactivityRecombinant chimeric antigensVisceral leishmaniasisCross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemicinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic |
| title |
Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic |
| spellingShingle |
Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic Daltro, Ramona Tavares Leishmaniose tegumentar americana Doença de Chagas Reatividade cruzada Antígenos quiméricos recombinantes Leishmaniose visceral American cutaneous leishmaniasis Chagas disease Cross-reactivity Recombinant chimeric antigens Visceral leishmaniasis |
| title_short |
Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic |
| title_full |
Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic |
| title_fullStr |
Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic |
| title_full_unstemmed |
Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic |
| title_sort |
Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic |
| author |
Daltro, Ramona Tavares |
| author_facet |
Daltro, Ramona Tavares Leony, Leonardo Maia Freitas, Natália Erdens Maron Silva, Ângelo Antônio Oliveira Santos, Emily Ferreira Del-Rei, Rodrigo Pimenta Brito, Maria Edileuza Felinto Brandão Filho, Sinval Pinto Gomes, Yara Miranda Silva, Marcelo Sousa Donato, Silvia Tavares Jeronimo, Selma Maria Bezerra Monteiro, Gloria Regina de Góis Carvalho, Lucas Pedreira Magalhães, Andréa Santos Zanchin, Nilson Ivo Tonin Celedon, Paola Alejandra Fiorani Santos, Fred Luciano Neves |
| author_role |
author |
| author2 |
Leony, Leonardo Maia Freitas, Natália Erdens Maron Silva, Ângelo Antônio Oliveira Santos, Emily Ferreira Del-Rei, Rodrigo Pimenta Brito, Maria Edileuza Felinto Brandão Filho, Sinval Pinto Gomes, Yara Miranda Silva, Marcelo Sousa Donato, Silvia Tavares Jeronimo, Selma Maria Bezerra Monteiro, Gloria Regina de Góis Carvalho, Lucas Pedreira Magalhães, Andréa Santos Zanchin, Nilson Ivo Tonin Celedon, Paola Alejandra Fiorani Santos, Fred Luciano Neves |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Daltro, Ramona Tavares Leony, Leonardo Maia Freitas, Natália Erdens Maron Silva, Ângelo Antônio Oliveira Santos, Emily Ferreira Del-Rei, Rodrigo Pimenta Brito, Maria Edileuza Felinto Brandão Filho, Sinval Pinto Gomes, Yara Miranda Silva, Marcelo Sousa Donato, Silvia Tavares Jeronimo, Selma Maria Bezerra Monteiro, Gloria Regina de Góis Carvalho, Lucas Pedreira Magalhães, Andréa Santos Zanchin, Nilson Ivo Tonin Celedon, Paola Alejandra Fiorani Santos, Fred Luciano Neves |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose tegumentar americana Doença de Chagas Reatividade cruzada Antígenos quiméricos recombinantes Leishmaniose visceral |
| topic |
Leishmaniose tegumentar americana Doença de Chagas Reatividade cruzada Antígenos quiméricos recombinantes Leishmaniose visceral American cutaneous leishmaniasis Chagas disease Cross-reactivity Recombinant chimeric antigens Visceral leishmaniasis |
| dc.subject.en.pt_BR.fl_str_mv |
American cutaneous leishmaniasis Chagas disease Cross-reactivity Recombinant chimeric antigens Visceral leishmaniasis |
| description |
O artigo encontra-se disponível em acesso aberto no site do Editor. |
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2019 |
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2019-08-01T14:02:44Z |
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2019-08-01T14:02:44Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
DALTRO, Ramona Tavares et al. Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic. Journal of Clinical Microbiology, v. 57, n. 8, p. 1-10, 2019. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/34560 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0095-1137 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/JCM.00762-19 |
| identifier_str_mv |
DALTRO, Ramona Tavares et al. Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic. Journal of Clinical Microbiology, v. 57, n. 8, p. 1-10, 2019. 0095-1137 10.1128/JCM.00762-19 |
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https://www.arca.fiocruz.br/handle/icict/34560 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
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American Society for Microbiology |
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