Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation

Detalhes bibliográficos
Autor(a) principal: Silva, Marialice da Fonseca Ferreira da
Data de Publicação: 2009
Outros Autores: Rodrigues, Renata Mendonça, Andrade, Elisabete Ferreira de, Carvalho, Laís de, Gross, Uwe, Lüder, Carsten G. K., Barbosa, Helene Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30859
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil / Georg-August University. Institute for Medical Microbiology. Göttingen, Germany.
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spelling Silva, Marialice da Fonseca Ferreira daRodrigues, Renata MendonçaAndrade, Elisabete Ferreira deCarvalho, Laís deGross, UweLüder, Carsten G. K.Barbosa, Helene Santos2019-01-03T15:54:50Z2019-01-03T15:54:50Z2009SILVA, Marialice da Fonseca Ferreira da; et al. Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation. Mem Inst Oswaldo Cruz, v.104, n.2, p.196-200, mar. 2009.https://www.arca.fiocruz.br/handle/icict/308591678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzToxoplasma GondiiConversão de PalcoCélula Muscular EsqueléticaInteração célula hospedeira de T. gondiiToxoplasma GondiiStage ConversionT. gondii-host cell interactionSkeletal Muscle CellTachyzoiteBradyzoiteSpontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil / Georg-August University. Institute for Medical Microbiology. Göttingen, Germany.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Georg-August University. Institute for Medical Microbiology. Göttingen, Germany.Georg-August University. Institute for Medical Microbiology. Göttingen, Germany.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.Although the predilection for Toxoplasma gondii to form cysts in the nervous system and skeletal and heart muscles has been described for more than fifty years, skeletal muscle cells (SkMCs) have not been explored as a host cell type to study the Toxoplasma-host cell interaction and investigate the intracellular development of the parasite. Morphological aspects of the initial events in the Toxoplasma-SkMC interaction were analysed and suggest that there are different processes of protozoan adhesion and invasion and of the subsequent fate of the parasite inside the parasitophorous vacuole (PV). Using scanning electron microscopy, Toxoplasma tachyzoites from the mouse-virulent RH strain were found to be attached to SkMCs by the anterior or posterior region of the body, with or without expansion of the SkMC membrane. This suggests that different types of parasite internalization occurred. Asynchronous multiplication and differentiation of T. gondii were observed. Importantly, intracellular parasites were seen to display high amounts of amylopectin granules in their cytoplasm, indicating that tachyzoites of the RH strain were able to differentiate spontaneously into bradyzoites in SkMCs. This stage conversion occurred in approximately 3% of the PVs. This is particularly intriguing as tachyzoites of virulent Toxoplasma strains are not thought to be prone to cyst formation. We discuss whether biological differences in host cells are crucial to Toxoplasma stage conversion and suggest that important questions concerning the host cell type and its relevance in Toxoplasma differentiation are still unanswered.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30859/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmariaaliceff_silva_etal_IOC_2009.pdfmariaaliceff_silva_etal_IOC_2009.pdfapplication/pdf817489https://www.arca.fiocruz.br/bitstream/icict/30859/2/mariaaliceff_silva_etal_IOC_2009.pdf7ef01761d8186eee0bc10abe68def94aMD52TEXTmariaaliceff_silva_etal_IOC_2009.pdf.txtmariaaliceff_silva_etal_IOC_2009.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation
title Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation
spellingShingle Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation
Silva, Marialice da Fonseca Ferreira da
Toxoplasma Gondii
Conversão de Palco
Célula Muscular Esquelética
Interação célula hospedeira de T. gondii
Toxoplasma Gondii
Stage Conversion
T. gondii-host cell interaction
Skeletal Muscle Cell
Tachyzoite
Bradyzoite
title_short Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation
title_full Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation
title_fullStr Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation
title_full_unstemmed Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation
title_sort Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation
author Silva, Marialice da Fonseca Ferreira da
author_facet Silva, Marialice da Fonseca Ferreira da
Rodrigues, Renata Mendonça
Andrade, Elisabete Ferreira de
Carvalho, Laís de
Gross, Uwe
Lüder, Carsten G. K.
Barbosa, Helene Santos
author_role author
author2 Rodrigues, Renata Mendonça
Andrade, Elisabete Ferreira de
Carvalho, Laís de
Gross, Uwe
Lüder, Carsten G. K.
Barbosa, Helene Santos
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Marialice da Fonseca Ferreira da
Rodrigues, Renata Mendonça
Andrade, Elisabete Ferreira de
Carvalho, Laís de
Gross, Uwe
Lüder, Carsten G. K.
Barbosa, Helene Santos
dc.subject.other.pt_BR.fl_str_mv Toxoplasma Gondii
Conversão de Palco
Célula Muscular Esquelética
Interação célula hospedeira de T. gondii
topic Toxoplasma Gondii
Conversão de Palco
Célula Muscular Esquelética
Interação célula hospedeira de T. gondii
Toxoplasma Gondii
Stage Conversion
T. gondii-host cell interaction
Skeletal Muscle Cell
Tachyzoite
Bradyzoite
dc.subject.en.pt_BR.fl_str_mv Toxoplasma Gondii
Stage Conversion
T. gondii-host cell interaction
Skeletal Muscle Cell
Tachyzoite
Bradyzoite
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil / Georg-August University. Institute for Medical Microbiology. Göttingen, Germany.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2019-01-03T15:54:50Z
dc.date.available.fl_str_mv 2019-01-03T15:54:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Marialice da Fonseca Ferreira da; et al. Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation. Mem Inst Oswaldo Cruz, v.104, n.2, p.196-200, mar. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30859
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SILVA, Marialice da Fonseca Ferreira da; et al. Spontaneous stage differentiation of mouse-virulent Toxoplasma gondii RH parasites in skeletal muscle cells: an ultrastructural evaluation. Mem Inst Oswaldo Cruz, v.104, n.2, p.196-200, mar. 2009.
1678-8060
url https://www.arca.fiocruz.br/handle/icict/30859
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30859/1/license.txt
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