Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro

Detalhes bibliográficos
Autor(a) principal: Guimarães, Erick Vaz
Data de Publicação: 2009
Outros Autores: Carvalho, Laís de, Barbosa, Helene Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30727
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.
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spelling Guimarães, Erick VazCarvalho, Laís deBarbosa, Helene Santos2018-12-20T15:45:48Z2018-12-20T15:45:48Z2009GUIMARÃES, Erick Vaz; CARVALHO, Laís de; BARBOSA, Helene Santos. Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro. Mem Inst Oswaldo Cruz, v.104, n.2, p.170-174, Mar. 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/307271678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzToxoplasma gondiicélulas musculares esqueléticascistogênesecistos teciduaisToxoplasma gondiitissue cystsbradyzoitescystogenesisskeletal muscle cellsInteraction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.Infection by the protozoan parasite Toxoplasma gondii is widely prevalent in humans and animals. To prevent human infection, all meat should be well cooked before consumption, since the parasite is present in skeletal muscle. In this context, the use of skeletal muscle cells (SkMCs) as a cellular model opens up new approaches to investigate T. gondii-host cell interactions. Immunofluorescent detection of proteins that are stage-specific for bradyzoites indicated that complete cystogenesis of T. gondii in in vitro cultures of SkMCs occurs after 96 h of infection. Ultrastructural analysis showed that, after 48 h of interaction, there were alterations on the parasitophorous vacuole membrane, including greater thickness and increased electron density at the inner face of the membrane. The present study demonstrates the potential use of primary cultures of SkMCs to evaluate different molecular aspects of T. gondii invasion and cystogenesis and presents a promising in vitro model for the screening of drug activities toward tissue cysts and bradyzoites.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30727/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALericguimaraes_helenebarbosa_etal_IOC_2009.pdfericguimaraes_helenebarbosa_etal_IOC_2009.pdfapplication/pdf801757https://www.arca.fiocruz.br/bitstream/icict/30727/2/ericguimaraes_helenebarbosa_etal_IOC_2009.pdffee6625f7ee25d83de0532c8f2c0c87eMD52TEXTericguimaraes_helenebarbosa_etal_IOC_2009.pdf.txtericguimaraes_helenebarbosa_etal_IOC_2009.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
title Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
spellingShingle Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
Guimarães, Erick Vaz
Toxoplasma gondii
células musculares esqueléticas
cistogênese
cistos teciduais
Toxoplasma gondii
tissue cysts
bradyzoites
cystogenesis
skeletal muscle cells
title_short Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
title_full Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
title_fullStr Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
title_full_unstemmed Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
title_sort Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
author Guimarães, Erick Vaz
author_facet Guimarães, Erick Vaz
Carvalho, Laís de
Barbosa, Helene Santos
author_role author
author2 Carvalho, Laís de
Barbosa, Helene Santos
author2_role author
author
dc.contributor.author.fl_str_mv Guimarães, Erick Vaz
Carvalho, Laís de
Barbosa, Helene Santos
dc.subject.other.pt_BR.fl_str_mv Toxoplasma gondii
células musculares esqueléticas
cistogênese
cistos teciduais
topic Toxoplasma gondii
células musculares esqueléticas
cistogênese
cistos teciduais
Toxoplasma gondii
tissue cysts
bradyzoites
cystogenesis
skeletal muscle cells
dc.subject.en.pt_BR.fl_str_mv Toxoplasma gondii
tissue cysts
bradyzoites
cystogenesis
skeletal muscle cells
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-12-20T15:45:48Z
dc.date.available.fl_str_mv 2018-12-20T15:45:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUIMARÃES, Erick Vaz; CARVALHO, Laís de; BARBOSA, Helene Santos. Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro. Mem Inst Oswaldo Cruz, v.104, n.2, p.170-174, Mar. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30727
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv GUIMARÃES, Erick Vaz; CARVALHO, Laís de; BARBOSA, Helene Santos. Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro. Mem Inst Oswaldo Cruz, v.104, n.2, p.170-174, Mar. 2009.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/30727
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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