Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study

Detalhes bibliográficos
Autor(a) principal: Pimenta, Rafael Nacif
Data de Publicação: 2020
Outros Autores: Pinto, Luciana da Conceição, Volfova, Vera, Volf, Petr, Pimenta, Paulo Filemon Paolucci, Secundino, Nagila Francinete Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43686
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil
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spelling Pimenta, Rafael NacifPinto, Luciana da ConceiçãoVolfova, VeraVolf, PetrPimenta, Paulo Filemon PaolucciSecundino, Nagila Francinete Costa2020-09-29T17:39:03Z2020-09-29T17:39:03Z2020PIMENTA, Rafael Nacif et al. Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study. Parasites Vectors , v. 13, 441, 2020. https://doi.org/10.1186/s13071-020-04311-y1756-3305https://www.arca.fiocruz.br/handle/icict/4368610.1186/s13071-020-04311-yengPubMed CentralConserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrasilDepartment of Parasitology, Charles University, Prague, Czech RepublicDepartment of Parasitology, Charles University, Prague, Czech RepublicFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrasilBackground: Sand flies are vectors of Leishmania spp., the causative agents of leishmaniasis in vertebrates, including man. The sand fly saliva contains powerful pharmacologically active substances that prevent hemostasis and enhance Leishmania spp. infections. On the other hand, salivary proteins can protect vaccinated mice challenged with parasites. Therefore, sand fly salivary proteins are relevant for the epidemiology of leishmaniasis and can be a potential target for a vaccine against leishmaniasis. Despite this, studies on sand fly salivary glands (SGs) are limited. Methods: The present study analyzes, in detail, the morphology, anatomy and ultrastructure of the SGs of sand fly vectors of the genera Lutzomyia and Phlebotomus. We used histology, transmission and scanning electron microscopy and lectin labeling associated with confocal laser microscopy. Results: The SGs have conserved and distinct morphological aspects according to the distinct sand fly species. Each SG has a single rounded lobe constituting of c.100–120 secretory cells. The SG secretory cells, according to their ultrastructure and lectin binding, were classified into five different subpopulations, which may differ in secretory pathways. Conclusions: To the best of our knowledge, these morphological details of sand fly salivary glands are described for the first time. Further studies are necessary to better understand the role of these different cell types and better relate them with the production and secretion of the saliva substances, which has a fundamental role in the interaction of the sand fly vectors with Leishmania.Secretory cell populationUltrastructureLectin bindingSand fly vectorsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/43686/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALConserved and distinct.pdfConserved and distinct.pdfapplication/pdf1620082https://www.arca.fiocruz.br/bitstream/icict/43686/2/Conserved%20and%20distinct.pdfb66577ea57819d40e61471516377dab5MD52TEXTConserved and distinct.pdf.txtConserved and distinct.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study
title Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study
spellingShingle Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study
Pimenta, Rafael Nacif
Secretory cell population
Ultrastructure
Lectin binding
Sand fly vectors
title_short Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study
title_full Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study
title_fullStr Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study
title_full_unstemmed Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study
title_sort Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study
author Pimenta, Rafael Nacif
author_facet Pimenta, Rafael Nacif
Pinto, Luciana da Conceição
Volfova, Vera
Volf, Petr
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
author_role author
author2 Pinto, Luciana da Conceição
Volfova, Vera
Volf, Petr
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pimenta, Rafael Nacif
Pinto, Luciana da Conceição
Volfova, Vera
Volf, Petr
Pimenta, Paulo Filemon Paolucci
Secundino, Nagila Francinete Costa
dc.subject.en.pt_BR.fl_str_mv Secretory cell population
Ultrastructure
Lectin binding
Sand fly vectors
topic Secretory cell population
Ultrastructure
Lectin binding
Sand fly vectors
description Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-09-29T17:39:03Z
dc.date.available.fl_str_mv 2020-09-29T17:39:03Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PIMENTA, Rafael Nacif et al. Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study. Parasites Vectors , v. 13, 441, 2020. https://doi.org/10.1186/s13071-020-04311-y
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43686
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.eissn.none.fl_str_mv 10.1186/s13071-020-04311-y
identifier_str_mv PIMENTA, Rafael Nacif et al. Conserved and distinct morphological aspects of the salivary glands of sand fly vectors of leishmaniasis: an anatomical and ultrastructural study. Parasites Vectors , v. 13, 441, 2020. https://doi.org/10.1186/s13071-020-04311-y
1756-3305
10.1186/s13071-020-04311-y
url https://www.arca.fiocruz.br/handle/icict/43686
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv PubMed Central
publisher.none.fl_str_mv PubMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/43686/1/license.txt
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