TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59746 https://doi.org/10.3390/ microorganisms11061558 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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Fragoso, Mariana Sayuri IshikawaSiqueira, Caroline Moraes deVitorino, Francisca Nathália LunaVieira, Alexandre ZanattaMartins-Duarte, Érica SantosFaoro, HelissonCunha, Júlia Pinheiro Chagas daÁvila, Andréa RodriguesNardelli, Sheila Cristina2023-07-25T14:36:11Z2023-07-25T14:36:11Z2023FRAGOSO, Mariana Sayuri Ishikawa et al. TgKDAC4: A unique deacetylase of Toxoplasma’s apicoplast. Microorganisms, v. 11, n. 1558, p. 1-16, 2023.2076-2607https://www.arca.fiocruz.br/handle/icict/59746https://doi.org/10.3390/ microorganisms11061558porMDPILisina desacetilasesTgKDAC4Lysine DeacetylasesApicoplastsApicoplastosApicoplastesToxoplasmaApicoplastosApicomplexaTgKDAC4: a unique deacetylase of Toxoplasma’s apicoplastinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Butantan. Centro de Toxinas, Resposta-Imune e Sinalização Celular -CeTICS. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Butantan. Centro de Toxinas, Resposta-Imune e Sinalização Celular -CeTICS. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa and causes toxoplasmosis infections, a disease that affects a quarter of the world’s population and has no effective cure. Epigenetic regulation is one of the mechanisms controlling gene expression and plays an essential role in all organisms. Lysine deacetylases (KDACs) act as epigenetic regulators affecting gene silencing in many eukaryotes. Here, we focus on TgKDAC4, an enzyme unique to apicomplexan parasites, and a class IV KDAC, the least-studied class of deacetylases so far. This enzyme shares only a portion of the specific KDAC domain with other organisms. Phylogenetic analysis from the TgKDAC4 domain shows a putative prokaryotic origin. Surprisingly, TgKDAC4 is located in the apicoplast, making it the only KDAC found in this organelle to date. Transmission electron microscopy assays confirmed the presence of TgKDAC4 in the periphery of the apicoplast. We identified possible targets or/and partners of TgKDAC4 by immunoprecipitation assays followed by mass spectrometry analysis, including TgCPN60 and TgGAPDH2, both located at the apicoplast and containing acetylation sites. Understanding how the protein works could provide new insights into the metabolism of the apicoplast, an essential organelle for parasite survival.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/59746/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmicroorganisms-11-015ok.pdfmicroorganisms-11-015ok.pdfapplication/pdf5575374https://www.arca.fiocruz.br/bitstream/icict/59746/2/microorganisms-11-015ok.pdf98209c79cf7c1f86c74f3341baa2305bMD52icict/597462023-07-25 11:36:11.961oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-25T14:36:11Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.en_US.fl_str_mv |
TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast |
title |
TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast |
spellingShingle |
TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast Fragoso, Mariana Sayuri Ishikawa Lisina desacetilases TgKDAC4 Lysine Deacetylases Apicoplasts Apicoplastos Apicoplastes Toxoplasma Apicoplastos Apicomplexa |
title_short |
TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast |
title_full |
TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast |
title_fullStr |
TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast |
title_full_unstemmed |
TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast |
title_sort |
TgKDAC4: a unique deacetylase of Toxoplasma’s apicoplast |
author |
Fragoso, Mariana Sayuri Ishikawa |
author_facet |
Fragoso, Mariana Sayuri Ishikawa Siqueira, Caroline Moraes de Vitorino, Francisca Nathália Luna Vieira, Alexandre Zanatta Martins-Duarte, Érica Santos Faoro, Helisson Cunha, Júlia Pinheiro Chagas da Ávila, Andréa Rodrigues Nardelli, Sheila Cristina |
author_role |
author |
author2 |
Siqueira, Caroline Moraes de Vitorino, Francisca Nathália Luna Vieira, Alexandre Zanatta Martins-Duarte, Érica Santos Faoro, Helisson Cunha, Júlia Pinheiro Chagas da Ávila, Andréa Rodrigues Nardelli, Sheila Cristina |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Fragoso, Mariana Sayuri Ishikawa Siqueira, Caroline Moraes de Vitorino, Francisca Nathália Luna Vieira, Alexandre Zanatta Martins-Duarte, Érica Santos Faoro, Helisson Cunha, Júlia Pinheiro Chagas da Ávila, Andréa Rodrigues Nardelli, Sheila Cristina |
dc.subject.other.en_US.fl_str_mv |
Lisina desacetilases TgKDAC4 |
topic |
Lisina desacetilases TgKDAC4 Lysine Deacetylases Apicoplasts Apicoplastos Apicoplastes Toxoplasma Apicoplastos Apicomplexa |
dc.subject.en.en_US.fl_str_mv |
Lysine Deacetylases Apicoplasts |
dc.subject.es.en_US.fl_str_mv |
Apicoplastos |
dc.subject.fr.en_US.fl_str_mv |
Apicoplastes |
dc.subject.decs.en_US.fl_str_mv |
Toxoplasma Apicoplastos Apicomplexa |
description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-07-25T14:36:11Z |
dc.date.available.fl_str_mv |
2023-07-25T14:36:11Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FRAGOSO, Mariana Sayuri Ishikawa et al. TgKDAC4: A unique deacetylase of Toxoplasma’s apicoplast. Microorganisms, v. 11, n. 1558, p. 1-16, 2023. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/59746 |
dc.identifier.issn.en_US.fl_str_mv |
2076-2607 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.3390/ microorganisms11061558 |
identifier_str_mv |
FRAGOSO, Mariana Sayuri Ishikawa et al. TgKDAC4: A unique deacetylase of Toxoplasma’s apicoplast. Microorganisms, v. 11, n. 1558, p. 1-16, 2023. 2076-2607 |
url |
https://www.arca.fiocruz.br/handle/icict/59746 https://doi.org/10.3390/ microorganisms11061558 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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MDPI |
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MDPI |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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