Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions

Detalhes bibliográficos
Autor(a) principal: Reis, Mônica Nogueira da Guarda
Data de Publicação: 2017
Outros Autores: Bello, Gonzalo, Guimarães, Monick Lindenmeyer, Stefani, Mariane Martins Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23084
Resumo: Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da AIDS e da Hanseníase. Goiânia, GO, Brasil.
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spelling Reis, Mônica Nogueira da GuardaBello, GonzaloGuimarães, Monick LindenmeyerStefani, Mariane Martins Araújo2017-11-09T10:50:52Z2017-11-09T10:50:52Z2017REIS, Mônica Nogueira da Guarda; et al. Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions. Plos One, v.12, n.6, e0178578, June 2017.1932-6203https://www.arca.fiocruz.br/handle/icict/2308410.1371/journal.pone.0178578engPublic Library of ScienceAIDSBrasilsequências do genomaCRFs_BF1AIDSBrazilCRFs_BF1genome sequencesCharacterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da AIDS e da Hanseníase. Goiânia, GO, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da AIDS e da Hanseníase. Goiânia, GO, Brasil.The Brazilian AIDS epidemic has been characterized by an increasing rate of BF1 recombinants and so far eight circulating recombinant forms/CRFs_BF1 have been described countrywide. In this study, pol sequences (protease/PR, reverse transcriptase/RT) of 87 BF1 mosaic isolates identified among 828 patients living in six Brazilian States from three geographic regions (Central West, North, Northeast) were analyzed. Phylogenetic and bootscan analyses were performed to investigate the evolutionary relationship and mosaic structure of BF1 isolates. Those analyses showed that 20.7% of mosaics (18 out of 87) were CRFs-like isolates, mostly represented by CRF28/CRF29_BF-like viruses (14 out of 18). We also identified five highly supported clusters that together comprise 42 out of 87 (48.3%) BF1 sequences, each cluster containing at least five sequences sharing a similar mosaic structure, suggesting possible new unidentified CRFs_BF1. The divergence time of these five potential new CRFs_BF1 clusters was estimated using a Bayesian approach and indicate that they probably originated between the middle 1980s and the middle 1990s. DNA was extracted from whole blood and four overlapping fragments were amplified by PCR providing full/near full length genomes (FLG/NFLG) and partial genomes. Eleven HIV-1 isolates from Cluster # 5 identified in epidemiologically unlinked individuals living in Central West and North regions provided FLG/NFLG/partial genome sequences with identical mosaic structure. These viruses differ from any known CRF_BF1 reported to date and were named CRF90_BF1 by the Los Alamos National Laboratory. This is the 9th CRF_BF1 described in Brazil and the first one identified in Central West and North regions. Our results highlight the importance of continued molecular screening and surveillance studies, especially of full genome sequences to understand the evolutionary dynamics of the HIV-1 epidemic in a country of continental dimensions as Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23084/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmonick_guimaraes_etal_IOC_2017.pdfmonick_guimaraes_etal_IOC_2017.pdfapplication/pdf9950005https://www.arca.fiocruz.br/bitstream/icict/23084/2/monick_guimaraes_etal_IOC_2017.pdfc3d6931a185786365a9879f40a02a18eMD52TEXTmonick_guimaraes_etal_IOC_2017.pdf.txtmonick_guimaraes_etal_IOC_2017.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions
title Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions
spellingShingle Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions
Reis, Mônica Nogueira da Guarda
AIDS
Brasil
sequências do genoma
CRFs_BF1
AIDS
Brazil
CRFs_BF1
genome sequences
title_short Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions
title_full Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions
title_fullStr Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions
title_full_unstemmed Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions
title_sort Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions
author Reis, Mônica Nogueira da Guarda
author_facet Reis, Mônica Nogueira da Guarda
Bello, Gonzalo
Guimarães, Monick Lindenmeyer
Stefani, Mariane Martins Araújo
author_role author
author2 Bello, Gonzalo
Guimarães, Monick Lindenmeyer
Stefani, Mariane Martins Araújo
author2_role author
author
author
dc.contributor.author.fl_str_mv Reis, Mônica Nogueira da Guarda
Bello, Gonzalo
Guimarães, Monick Lindenmeyer
Stefani, Mariane Martins Araújo
dc.subject.other.pt_BR.fl_str_mv AIDS
Brasil
sequências do genoma
CRFs_BF1
topic AIDS
Brasil
sequências do genoma
CRFs_BF1
AIDS
Brazil
CRFs_BF1
genome sequences
dc.subject.en.pt_BR.fl_str_mv AIDS
Brazil
CRFs_BF1
genome sequences
description Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da AIDS e da Hanseníase. Goiânia, GO, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-09T10:50:52Z
dc.date.available.fl_str_mv 2017-11-09T10:50:52Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REIS, Mônica Nogueira da Guarda; et al. Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions. Plos One, v.12, n.6, e0178578, June 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23084
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0178578
identifier_str_mv REIS, Mônica Nogueira da Guarda; et al. Characterization of HIV-1 CRF90_BF1 and putative novel CRFs_BF1 in Central West, North and Northeast Brazilian regions. Plos One, v.12, n.6, e0178578, June 2017.
1932-6203
10.1371/journal.pone.0178578
url https://www.arca.fiocruz.br/handle/icict/23084
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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