Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms

Detalhes bibliográficos
Autor(a) principal: Reis, Mônica N. G.
Data de Publicação: 2019
Outros Autores: Guimarães, Monick L., Bello, Gonzalo, Stefani, Mariane M. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32897
Resumo: Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da Aids e da Hanseníase. Goiânia, GO, Brasil.
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spelling Reis, Mônica N. G.Guimarães, Monick L.Bello, GonzaloStefani, Mariane M. A.2019-05-02T10:37:21Z2019-05-02T10:37:21Z2019REIS, Mônica N. G. et al. Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms. Frontiers in Microbiology, v. 10, Article 97, 13p, Feb. 2019.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/3289710.3389/fmicb.2019.00097engFrontiers MediaHIV-1Epidemiologia MolecularBrasil CentralHIV-1CRFsURFsmolecular epidemiologyCentral Western, BrazilIdentification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Formsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da Aids e da Hanseníase. Goiânia, GO, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da Aids e da Hanseníase. Goiânia, GO, Brasil.Intersubtype recombinants classified as circulating recombinant forms (CRFs) or unique recombinant forms (URFs) have been shown to play an important role in the complex and dynamic Brazilian HIV/AIDS epidemic. Previous pol region studies (2003-2013) in 828 patients from six states from Central Western, Northern and Northeastern Brazil reported variable rates of BF1, F1CB, BC, and CF1 mosaics. In this study HIV-1 subtype diversity BF1, F1CB, BC, and CF1 recombinants in pol were analyzed. Full/near-full/partial genome sequences were generated from F1CB and BF1 recombinants. Genomic DNA extracted from whole blood was used in nested-PCR to amplify four overlapping fragments encompassing the full HIV-1 genome. Phylogenetic trees were generated using the neighbor-joining/NJ method (MEGA 6.0). The time of the most recent common ancestor (TMRCA) of F1CB and BF1 clades was estimated using a Bayesian Markov Chain Monte Carlo approach (BEAST v1.8; BEAGLE). Bootscanning was used for recombination analyses (Simplot v3.5.1); separate NJ phylogenetic analysis of fragments confirmed subtypes. The phylogenetic analyses of protease/reverse-transcriptase sequences in 828 patients revealed 76% subtype B (n = 629), 6.4% subtype C (n = 53), 4.2% subtype F1 (n = 35), 13.4% intersubtype recombinants: 10.5% BF1 (n = 87), 2.3% BC (n = 19), 0.4% F1CB (n = 3), and 0.2% CF1 (n = 2). Two full and one partial BF1C genomes allowed the characterization of the CRF81_cpx that has 9 breakpoints dividing the genome into 10 subregions. Basic Local Alignment Search Tool searches (Los Alamos HIV Sequence Database) identified six other sequences with the same recombination profile in pol, five from Brazil, and one from Italy. The estimated median TMRCA of CRF81_cpx was 1999 (1992-2003). CRF60_BC-like sequences, originally described in Italy, were also found. Two full and one near full-length BF1 genomes led to the characterization of the new CRF99_BF1 that has six recombination breakpoints dividing the genome into seven subregions. Two new URFs BF1, with six recombination breakpoints and seven subregions were also characterized. The description of the first Brazilian BF1C CRF81_cpx and of the new CRF99_BF1 corroborate the important role of CRFs in the HIV/AIDS epidemic throughout Brazil. Our data also highlight the value of HIV-1 full-genome sequence studies in order to fully reveal the complexity of the epidemic in a huge country as Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32897/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMonicaNGReis_MarianeStefanni_etal_IOC_2019.pdfMonicaNGReis_MarianeStefanni_etal_IOC_2019.pdfapplication/pdf5869909https://www.arca.fiocruz.br/bitstream/icict/32897/2/MonicaNGReis_MarianeStefanni_etal_IOC_2019.pdf8d97a2f4f3df1e30398a8a7ff69fe57cMD52TEXTMonicaNGReis_MarianeStefanni_etal_IOC_2019.pdf.txtMonicaNGReis_MarianeStefanni_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms
title Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms
spellingShingle Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms
Reis, Mônica N. G.
HIV-1
Epidemiologia Molecular
Brasil Central
HIV-1
CRFs
URFs
molecular epidemiology
Central Western, Brazil
title_short Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms
title_full Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms
title_fullStr Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms
title_full_unstemmed Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms
title_sort Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms
author Reis, Mônica N. G.
author_facet Reis, Mônica N. G.
Guimarães, Monick L.
Bello, Gonzalo
Stefani, Mariane M. A.
author_role author
author2 Guimarães, Monick L.
Bello, Gonzalo
Stefani, Mariane M. A.
author2_role author
author
author
dc.contributor.author.fl_str_mv Reis, Mônica N. G.
Guimarães, Monick L.
Bello, Gonzalo
Stefani, Mariane M. A.
dc.subject.other.pt_BR.fl_str_mv HIV-1
Epidemiologia Molecular
Brasil Central
topic HIV-1
Epidemiologia Molecular
Brasil Central
HIV-1
CRFs
URFs
molecular epidemiology
Central Western, Brazil
dc.subject.en.pt_BR.fl_str_mv HIV-1
CRFs
URFs
molecular epidemiology
Central Western, Brazil
description Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da Aids e da Hanseníase. Goiânia, GO, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-05-02T10:37:21Z
dc.date.available.fl_str_mv 2019-05-02T10:37:21Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REIS, Mônica N. G. et al. Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms. Frontiers in Microbiology, v. 10, Article 97, 13p, Feb. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32897
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2019.00097
identifier_str_mv REIS, Mônica N. G. et al. Identification of New HIV-1 Circulating Recombinant Forms CRF81_cpx and CRF99_BF1 in Central Western Brazil and of Unique BF1 Recombinant Forms. Frontiers in Microbiology, v. 10, Article 97, 13p, Feb. 2019.
1664-302X
10.3389/fmicb.2019.00097
url https://www.arca.fiocruz.br/handle/icict/32897
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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