Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice

Detalhes bibliográficos
Autor(a) principal: Zanini, Graziela M.
Data de Publicação: 2011
Outros Autores: Cabrales, Pedro, Barkho, Wisam, Frangos, John A., Carvalho, Leonardo J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1186/1742-2094-8-66
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33943
Resumo: La Jolla Bioengineering Institute. San Diego, CA, USA / Fundação Oswaldo Cruz. Instituto de Pesquisas Clinicas Evandro Chagas. Serviço de Parasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Zanini, Graziela M.Cabrales, PedroBarkho, WisamFrangos, John A.Carvalho, Leonardo J. M.2019-07-10T13:40:09Z2019-07-10T13:40:09Z2011CABRALES, Pedro et al. Nitric oxide protection against murine cerebral malaria is associated with improved cerebral microcirculatory physiology. Journal of Neuroinflammation, v. 203, p. 1454-1463, 2011.1742-2094https://www.arca.fiocruz.br/handle/icict/3394310.1186/1742-2094-8-661742-2094engBMCExogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLa Jolla Bioengineering Institute. San Diego, CA, USA / Fundação Oswaldo Cruz. Instituto de Pesquisas Clinicas Evandro Chagas. Serviço de Parasitologia. Rio de Janeiro, RJ, Brasil.La Jolla Bioengineering Institute. San Diego, CA, USA / University of California. Department of Bioengineering. San Diego, CA, USA.La Jolla Bioengineering Institute. San Diego, CA, USA.La Jolla Bioengineering Institute. San Diego, CA, USA.La Jolla Bioengineering Institute. San Diego, CA, USA.Background: Cerebral malaria (CM) is a lethal complication of Plasmodium falciparum infections. In the Plasmodium berghei ANKA (PbA) murine model, CM is associated with marked brain inflammation, increased expression of endothelial cell adhesion molecules and leukocyte and platelet accumulation in brain vessels, causing vascular occlusion and decreased blood flow, damaging the endothelium and leading to blood-brain barrier breakdown, leakage and hemorrhages. Exogenous nitric oxide (NO) administration largely prevents the syndrome. Here we evaluated whether the mechanism of action of NO in preventing murine CM is related to its antiinflammatory properties and to protection of the endothelium. Methods: C57Bl/6 mice infected with PbA were treated twice a day with saline or dipropylenetriamineNONOate (DPTA-NO). Endothelial cell adhesion molecule (ICAM-1, VCAM, E- and P-selectin) expression in brain tissue on day 6 of infection was assessed in both groups by western blot. For intravital microscopy studies, DPTA-NO-treated and saline-treated mice with a previously implanted closed cranial window were injected with albumin-FITC, anti-CD45-TxR and anti-CD41-FITC antibodies on day 6 of infection for quantification of albumin leakage, leukocyte and platelet adherence in pial vessels. Results: PbA-infected mice treated with the NO-donor DPTA-NO showed decreased expression of ICAM-1 and P-selectin, but not VCAM-1, in the brain, compared to saline-treated mice. DPTA-NO treatment also decreased the number of adherent leukocytes and platelets in pial vessels, particularly in venules 30-50 μm in diameter, decreased inflammatory vascular resistance and prevented the occurrence of arteriolar and venular albumin leakage observed in saline-treated PbA-infected mice, as assessed by intravital microscopy. Conclusions: These results indicate that the protective effect of exogenous NO on murine CM is associated with decreased brain vascular expression of inflammatory markers resulting in attenuated endothelial junction damage and facilitating blood flow.Cerebral malariaExogenous nitric oxideBrain vascular inflammationPlasmodium berghei ANKAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83101https://www.arca.fiocruz.br/bitstream/icict/33943/1/license.txt63df43feba17d7507b32704290dbf79aMD51ORIGINALExogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice..pdfExogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice..pdfapplication/pdf387883https://www.arca.fiocruz.br/bitstream/icict/33943/2/Exogenous%20nitric%20oxide%20decreases%20brain%20vascular%20inflammation%2c%20leakage%20and%20venular%20resistance%20during%20Plasmodium%20berghei%20ANKA%20infection%20in%20mice..pdfb70226110d9d41af2fcb6f4e8358f025MD52TEXTExogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice..pdf.txtExogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice..pdf.txtExtracted texttext/plain43048https://www.arca.fiocruz.br/bitstream/icict/33943/3/Exogenous%20nitric%20oxide%20decreases%20brain%20vascular%20inflammation%2c%20leakage%20and%20venular%20resistance%20during%20Plasmodium%20berghei%20ANKA%20infection%20in%20mice..pdf.txtc20c0cf7c942bdb00a529a1cf3458c6dMD53icict/339432019-07-11 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dc.title.pt_BR.fl_str_mv Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice
title Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice
spellingShingle Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice
Zanini, Graziela M.
Cerebral malaria
Exogenous nitric oxide
Brain vascular inflammation
Plasmodium berghei ANKA
title_short Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice
title_full Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice
title_fullStr Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice
title_full_unstemmed Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice
title_sort Exogenous nitric oxide decreases brain vascular inflammation, leakage and venular resistance during Plasmodium berghei ANKA infection in mice
author Zanini, Graziela M.
author_facet Zanini, Graziela M.
Cabrales, Pedro
Barkho, Wisam
Frangos, John A.
Carvalho, Leonardo J. M.
author_role author
author2 Cabrales, Pedro
Barkho, Wisam
Frangos, John A.
Carvalho, Leonardo J. M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Zanini, Graziela M.
Cabrales, Pedro
Barkho, Wisam
Frangos, John A.
Carvalho, Leonardo J. M.
dc.subject.en.pt_BR.fl_str_mv Cerebral malaria
Exogenous nitric oxide
Brain vascular inflammation
Plasmodium berghei ANKA
topic Cerebral malaria
Exogenous nitric oxide
Brain vascular inflammation
Plasmodium berghei ANKA
description La Jolla Bioengineering Institute. San Diego, CA, USA / Fundação Oswaldo Cruz. Instituto de Pesquisas Clinicas Evandro Chagas. Serviço de Parasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2019-07-10T13:40:09Z
dc.date.available.fl_str_mv 2019-07-10T13:40:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CABRALES, Pedro et al. Nitric oxide protection against murine cerebral malaria is associated with improved cerebral microcirculatory physiology. Journal of Neuroinflammation, v. 203, p. 1454-1463, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33943
dc.identifier.issn.pt_BR.fl_str_mv 1742-2094
dc.identifier.doi.none.fl_str_mv 10.1186/1742-2094-8-66
dc.identifier.eissn.none.fl_str_mv 1742-2094
identifier_str_mv CABRALES, Pedro et al. Nitric oxide protection against murine cerebral malaria is associated with improved cerebral microcirculatory physiology. Journal of Neuroinflammation, v. 203, p. 1454-1463, 2011.
1742-2094
10.1186/1742-2094-8-66
url https://www.arca.fiocruz.br/handle/icict/33943
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33943/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33943/2/Exogenous%20nitric%20oxide%20decreases%20brain%20vascular%20inflammation%2c%20leakage%20and%20venular%20resistance%20during%20Plasmodium%20berghei%20ANKA%20infection%20in%20mice..pdf
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