Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/22843 |
Resumo: | University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA. |
| id |
CRUZ_0ea5ea61336a8e93c648511e0f739f29 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/22843 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Magnani, Diogo M.Rogers, Thomas F.Beutler, NathanRicciardi, Michael J.Bailey, Varian K.Gonzalez Nieto, LucasBriney, BryanSok, DevinLe, KhoaStrubel, AlexanderGutman, Martin J.Pedreño-Lopez, NúriaGrubaugh, Nathan D.Silveira, Cassia G. T.Maxwell, Helen S.Domingues, AlineMartins, Mauricio A.Lee, David E.Okwuazi, Erica E.Jean, SherrieStrobert, Elizabeth A.Chahroudi, AnnSilvestri, GuidoVanderford, Thomas H.Kallas, Esper GeorgesDesrosiers, Ronald C.Bonaldo, Myrna C.Whitehead, Stephen S.Burton, Dennis R.Watkins, David I.2017-10-17T14:29:46Z2017-10-17T14:29:46Z2017MAGNANI, Diogo M. et al. Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques. Science Translational Medicine, n.9, eaan8184, 8p, Oct. 2017.1946-6234https://www.arca.fiocruz.br/handle/icict/2284310.1126/scitranslmed.aan81841946-6242engAmerican Association for the Advancement of ScienceZika virusInfecçãoPrevençãoAnticorpos Monoclonais neutralizantesZika virusinfectionpreventionneutralizing monoclonal antibodiesNeutralizing human monoclonal antibodies prevent Zika virus infection in macaquesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.The Scripps Research Institute. International AIDS Vaccine Initiative Neutralizing Antibody Center. Department of Immunology and Microbiology. La Jolla, CA, USA.The Scripps Research Institute. International AIDS Vaccine Initiative Neutralizing Antibody Center. Department of Immunology and Microbiology. La Jolla, CA, USA.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.The Scripps Research Institute. International AIDS Vaccine Initiative Neutralizing Antibody Center. Department of Immunology and Microbiology. La Jolla, CA, USA;.The Scripps Research Institute. International AIDS Vaccine Initiative Neutralizing Antibody Center. Department of Immunology and Microbiology. La Jolla, CA, USA;.The Scripps Research Institute. International AIDS Vaccine Initiative Neutralizing Antibody Center. Department of Immunology and Microbiology. La Jolla, CA, USA;.The Scripps Research Institute. International AIDS Vaccine Initiative Neutralizing Antibody Center. Department of Immunology and Microbiology. La Jolla, CA, USA;.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.The Scripps Research Institute. International AIDS Vaccine Initiative Neutralizing Antibody Center. Department of Immunology and Microbiology. La Jolla, CA, USA;.Universidade de São Paulo. Escola de Medicina. Divisão de Imunologia Clínica e Alergia. São Paulo, SP, Brasil.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.Emory University. YerkesNational Primate Research Center. Atlanta, GA, USA.Emory University. YerkesNational Primate Research Center. Atlanta, GA, USA.Emory University. YerkesNational Primate Research Center. Atlanta, GA, USA.Emory University. YerkesNational Primate Research Center. Atlanta, GA, USA.Emory University. YerkesNational Primate Research Center. Atlanta, GA, USA / Emory University. Department of Pediatrics. Atlanta, GA, USA.Emory University. YerkesNational Primate Research Center. Atlanta, GA, USA.Emory University. YerkesNational Primate Research Center. Atlanta, GA, USA.Universidade de São Paulo. Escola de Medicina. Divisão de Imunologia Clínica e Alergia. São Paulo, SP, Brasil.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Infectious Diseases. Bethesda, MD, USA.The Scripps Research Institute. International AIDS Vaccine Initiative Neutralizing Antibody Center. Department of Immunology and Microbiology. La Jolla, CA, USA / Ragon Institute of MGH, MIT and Harvard. Cambridge, MA, USA.University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA.Therapies to prevent maternal Zika virus (ZIKV) infection and its subsequent fetal developmental complications are urgently required. We isolated three potent ZIKV-neutralizing monoclonal antibodies (nmAbs) from the plasmablasts of a ZIKV-infected patient-SMZAb1, SMZAb2, and SMZAb5-directed against two different domains of the virus. We engineered these nmAbs with Fc LALA mutations that abrogate Fcγ receptor binding, thus eliminating potential therapy-mediated antibody-dependent enhancement. We administered a cocktail of these three nmAbs to nonhuman primates 1 day before challenge with ZIKV and demonstrated that the nmAbs completely prevented viremia in serum after challenge. Given that numerous antibodies have exceptional safety profiles in humans, the cocktail described here could be rapidly developed to protect uninfected pregnant women and their fetuses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/22843/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmyrna2_bonaldo_etal_IOC_2017.pdfmyrna2_bonaldo_etal_IOC_2017.pdfapplication/pdf474211https://www.arca.fiocruz.br/bitstream/icict/22843/2/myrna2_bonaldo_etal_IOC_2017.pdf8667032b81344ed289561d50d9b7d007MD52TEXTmyrna2_bonaldo_etal_IOC_2017.pdf.txtmyrna2_bonaldo_etal_IOC_2017.pdf.txtExtracted texttext/plain48988https://www.arca.fiocruz.br/bitstream/icict/22843/3/myrna2_bonaldo_etal_IOC_2017.pdf.txt4a7965c6a0264b419eac40129a715e6eMD53icict/228432022-06-24 12:19:38.782oai:www.arca.fiocruz.br:icict/22843Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:19:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en.fl_str_mv |
Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques |
| title |
Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques |
| spellingShingle |
Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques Magnani, Diogo M. Zika virus Infecção Prevenção Anticorpos Monoclonais neutralizantes Zika virus infection prevention neutralizing monoclonal antibodies |
| title_short |
Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques |
| title_full |
Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques |
| title_fullStr |
Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques |
| title_full_unstemmed |
Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques |
| title_sort |
Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques |
| author |
Magnani, Diogo M. |
| author_facet |
Magnani, Diogo M. Rogers, Thomas F. Beutler, Nathan Ricciardi, Michael J. Bailey, Varian K. Gonzalez Nieto, Lucas Briney, Bryan Sok, Devin Le, Khoa Strubel, Alexander Gutman, Martin J. Pedreño-Lopez, Núria Grubaugh, Nathan D. Silveira, Cassia G. T. Maxwell, Helen S. Domingues, Aline Martins, Mauricio A. Lee, David E. Okwuazi, Erica E. Jean, Sherrie Strobert, Elizabeth A. Chahroudi, Ann Silvestri, Guido Vanderford, Thomas H. Kallas, Esper Georges Desrosiers, Ronald C. Bonaldo, Myrna C. Whitehead, Stephen S. Burton, Dennis R. Watkins, David I. |
| author_role |
author |
| author2 |
Rogers, Thomas F. Beutler, Nathan Ricciardi, Michael J. Bailey, Varian K. Gonzalez Nieto, Lucas Briney, Bryan Sok, Devin Le, Khoa Strubel, Alexander Gutman, Martin J. Pedreño-Lopez, Núria Grubaugh, Nathan D. Silveira, Cassia G. T. Maxwell, Helen S. Domingues, Aline Martins, Mauricio A. Lee, David E. Okwuazi, Erica E. Jean, Sherrie Strobert, Elizabeth A. Chahroudi, Ann Silvestri, Guido Vanderford, Thomas H. Kallas, Esper Georges Desrosiers, Ronald C. Bonaldo, Myrna C. Whitehead, Stephen S. Burton, Dennis R. Watkins, David I. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Magnani, Diogo M. Rogers, Thomas F. Beutler, Nathan Ricciardi, Michael J. Bailey, Varian K. Gonzalez Nieto, Lucas Briney, Bryan Sok, Devin Le, Khoa Strubel, Alexander Gutman, Martin J. Pedreño-Lopez, Núria Grubaugh, Nathan D. Silveira, Cassia G. T. Maxwell, Helen S. Domingues, Aline Martins, Mauricio A. Lee, David E. Okwuazi, Erica E. Jean, Sherrie Strobert, Elizabeth A. Chahroudi, Ann Silvestri, Guido Vanderford, Thomas H. Kallas, Esper Georges Desrosiers, Ronald C. Bonaldo, Myrna C. Whitehead, Stephen S. Burton, Dennis R. Watkins, David I. |
| dc.subject.other.pt_BR.fl_str_mv |
Zika virus Infecção Prevenção Anticorpos Monoclonais neutralizantes |
| topic |
Zika virus Infecção Prevenção Anticorpos Monoclonais neutralizantes Zika virus infection prevention neutralizing monoclonal antibodies |
| dc.subject.en.en.fl_str_mv |
Zika virus infection prevention neutralizing monoclonal antibodies |
| description |
University of Miami Leonard M. Miller School of Medicine. Department of Pathology. Miami, FL, USA. |
| publishDate |
2017 |
| dc.date.accessioned.fl_str_mv |
2017-10-17T14:29:46Z |
| dc.date.available.fl_str_mv |
2017-10-17T14:29:46Z |
| dc.date.issued.fl_str_mv |
2017 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
MAGNANI, Diogo M. et al. Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques. Science Translational Medicine, n.9, eaan8184, 8p, Oct. 2017. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/22843 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1946-6234 |
| dc.identifier.doi.none.fl_str_mv |
10.1126/scitranslmed.aan8184 |
| dc.identifier.eissn.pt_BR.fl_str_mv |
1946-6242 |
| identifier_str_mv |
MAGNANI, Diogo M. et al. Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques. Science Translational Medicine, n.9, eaan8184, 8p, Oct. 2017. 1946-6234 10.1126/scitranslmed.aan8184 1946-6242 |
| url |
https://www.arca.fiocruz.br/handle/icict/22843 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
American Association for the Advancement of Science |
| publisher.none.fl_str_mv |
American Association for the Advancement of Science |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/22843/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/22843/2/myrna2_bonaldo_etal_IOC_2017.pdf https://www.arca.fiocruz.br/bitstream/icict/22843/3/myrna2_bonaldo_etal_IOC_2017.pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 8667032b81344ed289561d50d9b7d007 4a7965c6a0264b419eac40129a715e6e |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813008934847381504 |