Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.molimm.2013.05.231 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30416 |
Resumo: | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil |
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Souza, Juliana VitorianoMoreira, Nádia das DoresSouza, Daniel MenezesRoatt, Bruno MendesSoares, Rodrigo Diande Oliveira AguiarMathias, Fernando Augusto SiqueiraCardoso, Jamille Mirellede OliveiraGiunchetti, Rodolfo CordeiroSá, Renata Guerra deOliveira, Rodrigo Corrêa deCarneiro, Cláudia MartinsReis, Alexandre Barbosa2018-12-07T12:39:10Z2018-12-07T12:39:10Z2013SOUZA, Juliana Vitoriano et al. Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis. Molecular Immunology, v. 56, p. 540-548, 20130161-5890https://www.arca.fiocruz.br/handle/icict/3041610.1016/j.molimm.2013.05.231engElsevier Ltd.Leishmaniose visceral caninaQuimiocinasSaponinaAdjuvanteCanine visceral leishmaniasisChemokinesSaponinAdjuvantDogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilFundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil/Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Bioquímica e Biologia Molecular. Ouro Preto, MG, BrazilFundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto. Ouro Preto, MG, Brazil/Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais - INCT-DT, CNPq. BrazilThe complex interplay between cytokines and chemokines regulates innate and adaptive immuneresponses against pathogens; specifically, cytokine and chemokine expression drives activation ofimmune effector cells and their recruitment to tissue infection sites. Herein, we inoculated dogs withLeishmania braziliensis antigens plus saponin (the LBSap vaccine), as well as with the vaccine components,and then used real-time PCR to evaluate the kinetics of dermal expression of mRNAs of cytokines (IL-12,IFN- _, TNF- _, IL-4, IL-13, TGF- _ and IL-10) and chemokines (CCL2, CCL4, CCL5, CCL21 and CXCL8) 1, 12, 24and 48 h after inoculation. We also evaluated the correlation between cytokine and chemokine expres-sion and dermal cellularity. The LBSap vaccine induced high levels of IL-12 and IL-10 expression at 12 and24 h, respectively. Furthermore, we observed positive correlations between IL-12 and IL-13 expression,IFN- _ and IL-13 expression, and IL-13 and TGF- _ expression, suggesting that a mixed cytokine microen-vironment developed after immunization with the vaccine. Inoculation with the saponin adjuvant aloneinduced a chemokine and cytokine expression profile similar to that observed in the LBSap group. CCL4and CXCL8 chemokine expression was up regulated by the LBSap vaccine. CCL5 expression was initiallyhighest in the LBSap group, but at 48 h, expression was highest in the LB group. Information about thekinetics of the immune response to this vaccine gained using this dog model will help to elucidate themechanisms of and factors involved in a protective response against Leishmania infection and will aid inestablishing rational approaches for the development of vaccines against canine visceral leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30416/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALDogs immunized with LBSap vaccine.pdfDogs immunized with LBSap vaccine.pdfapplication/pdf667287https://www.arca.fiocruz.br/bitstream/icict/30416/2/Dogs%20immunized%20with%20LBSap%20vaccine.pdfca80e6533245e44ba408a15e2745a09fMD52TEXTDogs immunized with LBSap vaccine.pdf.txtDogs immunized with LBSap vaccine.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis |
| title |
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis |
| spellingShingle |
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis Souza, Juliana Vitoriano Leishmaniose visceral canina Quimiocinas Saponina Adjuvante Canine visceral leishmaniasis Chemokines Saponin Adjuvant |
| title_short |
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis |
| title_full |
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis |
| title_fullStr |
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis |
| title_full_unstemmed |
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis |
| title_sort |
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis |
| author |
Souza, Juliana Vitoriano |
| author_facet |
Souza, Juliana Vitoriano Moreira, Nádia das Dores Souza, Daniel Menezes Roatt, Bruno Mendes Soares, Rodrigo Diande Oliveira Aguiar Mathias, Fernando Augusto Siqueira Cardoso, Jamille Mirellede Oliveira Giunchetti, Rodolfo Cordeiro Sá, Renata Guerra de Oliveira, Rodrigo Corrêa de Carneiro, Cláudia Martins Reis, Alexandre Barbosa |
| author_role |
author |
| author2 |
Moreira, Nádia das Dores Souza, Daniel Menezes Roatt, Bruno Mendes Soares, Rodrigo Diande Oliveira Aguiar Mathias, Fernando Augusto Siqueira Cardoso, Jamille Mirellede Oliveira Giunchetti, Rodolfo Cordeiro Sá, Renata Guerra de Oliveira, Rodrigo Corrêa de Carneiro, Cláudia Martins Reis, Alexandre Barbosa |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Souza, Juliana Vitoriano Moreira, Nádia das Dores Souza, Daniel Menezes Roatt, Bruno Mendes Soares, Rodrigo Diande Oliveira Aguiar Mathias, Fernando Augusto Siqueira Cardoso, Jamille Mirellede Oliveira Giunchetti, Rodolfo Cordeiro Sá, Renata Guerra de Oliveira, Rodrigo Corrêa de Carneiro, Cláudia Martins Reis, Alexandre Barbosa |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose visceral canina Quimiocinas Saponina Adjuvante |
| topic |
Leishmaniose visceral canina Quimiocinas Saponina Adjuvante Canine visceral leishmaniasis Chemokines Saponin Adjuvant |
| dc.subject.en.pt_BR.fl_str_mv |
Canine visceral leishmaniasis Chemokines Saponin Adjuvant |
| description |
Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil |
| publishDate |
2013 |
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2013 |
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2018-12-07T12:39:10Z |
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2018-12-07T12:39:10Z |
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info:eu-repo/semantics/article |
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SOUZA, Juliana Vitoriano et al. Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis. Molecular Immunology, v. 56, p. 540-548, 2013 |
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https://www.arca.fiocruz.br/handle/icict/30416 |
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0161-5890 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.molimm.2013.05.231 |
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SOUZA, Juliana Vitoriano et al. Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokinesin the dermis. Molecular Immunology, v. 56, p. 540-548, 2013 0161-5890 10.1016/j.molimm.2013.05.231 |
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Elsevier Ltd. |
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Elsevier Ltd. |
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