An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16401 |
Resumo: | University of Texas Medical Branch (UTMB). Department of Internal Medicine. Division of Infectious Diseases. Galveston, Texas, USA. |
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Saldarriaga, Omar A.Castellanos-Gonzalez, AlejandroPorrozzi, RenatoBaldeviano, Gerald C.Lescano, Andrés G.Los Santos, Maxy B. deFernandez, Olga L.Saravia, Nancy G.Costa, ErikaMelby, Peter C.Travi, Bruno L.2016-12-01T14:12:55Z2016-12-01T14:12:55Z2016SAIDARRIAGA, Omar A. et al. An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections. PLoS Negl Trop Dis , v.10, n.4, e0004638, 12p, Apr. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1640110.1371/journal. pntd.0004638engPublic Library of ScienceTeste MolecularLeishmania cutâneaDiagnósticoCutaneous Leishmania Viannia spp.Molecular TestDiagnosisAn Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Texas Medical Branch (UTMB). Department of Internal Medicine. Division of Infectious Diseases. Galveston, Texas, USA.University of Texas Medical Branch (UTMB). Department of Internal Medicine. Division of Infectious Diseases. Galveston, Texas, USA / University of Texas Medical Branch (UTMB),. Center for Tropical Diseases (CTD). Galveston, Texas, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.U.S. Naval Medical Research Unit No. 6 (NAMRU-6). Lima, Peru.U.S. Naval Medical Research Unit No. 6 (NAMRU-6). Lima, Peru / Universidad Peruana Cayetano Heredia. Lima, Peru.U.S. Naval Medical Research Unit No. 6 (NAMRU-6). Lima, Peru.Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM). Cali, Colombia.Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM). Cali, Colombia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil.University of Texas Medical Branch (UTMB). Department of Internal Medicine. Division of Infectious Diseases. Galveston, Texas, USA / University of Texas Medical Branch (UTMB),. Center for Tropical Diseases (CTD). Galveston, Texas, USA / University of Texas Medical Branch (UTMB). Department of Microbiology and Immunology. Galveston, Texas, USA.University of Texas Medical Branch (UTMB). Department of Internal Medicine. Division of Infectious Diseases. Galveston, Texas, USA / University of Texas Medical Branch (UTMB),. Center for Tropical Diseases (CTD). Galveston, Texas, USA / University of Texas Medical Branch (UTMB). Department of Microbiology and Immunology. Galveston, Texas, USA.Cutaneous and mucosal leishmaniasis is widely distributed in Central and South America. Leishmania of the Viannia subgenus are the most frequent species infecting humans. L. (V.) braziliensis, L. (V.) panamensis are also responsible for metastatic mucosal leishmaniasis. Conventional or real time PCR is a more sensitive diagnostic test than microscopy, but the cost and requirement for infrastructure and trained personnel makes it impractical in most endemic regions. Primary health systems need a sensitive and specific point of care (POC) diagnostic tool. We developed a novel POC molecular diagnostic test for cutaneous leishmaniasis caused by Leishmania (Viannia) spp. Parasite DNA was amplified using isothermal Recombinase Polymerase Amplification (RPA) with primers and probes that targeted the kinetoplast DNA. The amplification product was detected by naked eye with a lateral flow (LF) immunochromatographic strip. The RPA-LF had an analytical sensitivity equivalent to 0.1 parasites per reaction. The test amplified the principal L. Viannia species from multiple countries: L. (V.) braziliensis (n = 33), L. (V.) guyanensis (n = 17), L. (V.) panamensis (n = 9). The less common L. (V.) lainsoni, L. (V.) shawi, and L. (V.) naiffi were also amplified. No amplification was observed in parasites of the L. (Leishmania) subgenus. In a small number of clinical samples (n = 13) we found 100% agreement between PCR and RPA-LF. The high analytical sensitivity and clinical validation indicate the test could improve the efficiency of diagnosis, especially in chronic lesions with submicroscopic parasite burdens. Field implementation of the RPA-LF test could contribute to management and control of cutaneous and mucosal leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83134https://www.arca.fiocruz.br/bitstream/icict/16401/1/license.txt0ab46789d568c4ba27c7b5d29a9fe9c4MD51ORIGINALerika_costa_etal_IOC_2016.pdferika_costa_etal_IOC_2016.pdfapplication/pdf1981042https://www.arca.fiocruz.br/bitstream/icict/16401/2/erika_costa_etal_IOC_2016.pdf593f9a59432e284a1467ef8382663a08MD52TEXTerika_costa_etal_IOC_2016.pdf.txterika_costa_etal_IOC_2016.pdf.txtExtracted texttext/plain36950https://www.arca.fiocruz.br/bitstream/icict/16401/3/erika_costa_etal_IOC_2016.pdf.txtc5060e9ff237d7b97835e74b65b4b28eMD53icict/164012018-08-15 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| dc.title.pt_BR.fl_str_mv |
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections |
| title |
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections |
| spellingShingle |
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections Saldarriaga, Omar A. Teste Molecular Leishmania cutânea Diagnóstico Cutaneous Leishmania Viannia spp. Molecular Test Diagnosis |
| title_short |
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections |
| title_full |
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections |
| title_fullStr |
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections |
| title_full_unstemmed |
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections |
| title_sort |
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections |
| author |
Saldarriaga, Omar A. |
| author_facet |
Saldarriaga, Omar A. Castellanos-Gonzalez, Alejandro Porrozzi, Renato Baldeviano, Gerald C. Lescano, Andrés G. Los Santos, Maxy B. de Fernandez, Olga L. Saravia, Nancy G. Costa, Erika Melby, Peter C. Travi, Bruno L. |
| author_role |
author |
| author2 |
Castellanos-Gonzalez, Alejandro Porrozzi, Renato Baldeviano, Gerald C. Lescano, Andrés G. Los Santos, Maxy B. de Fernandez, Olga L. Saravia, Nancy G. Costa, Erika Melby, Peter C. Travi, Bruno L. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Saldarriaga, Omar A. Castellanos-Gonzalez, Alejandro Porrozzi, Renato Baldeviano, Gerald C. Lescano, Andrés G. Los Santos, Maxy B. de Fernandez, Olga L. Saravia, Nancy G. Costa, Erika Melby, Peter C. Travi, Bruno L. |
| dc.subject.other.pt_BR.fl_str_mv |
Teste Molecular Leishmania cutânea Diagnóstico |
| topic |
Teste Molecular Leishmania cutânea Diagnóstico Cutaneous Leishmania Viannia spp. Molecular Test Diagnosis |
| dc.subject.en.pt_BR.fl_str_mv |
Cutaneous Leishmania Viannia spp. Molecular Test Diagnosis |
| description |
University of Texas Medical Branch (UTMB). Department of Internal Medicine. Division of Infectious Diseases. Galveston, Texas, USA. |
| publishDate |
2016 |
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2016-12-01T14:12:55Z |
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2016-12-01T14:12:55Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SAIDARRIAGA, Omar A. et al. An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections. PLoS Negl Trop Dis , v.10, n.4, e0004638, 12p, Apr. 2016. |
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https://www.arca.fiocruz.br/handle/icict/16401 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal. pntd.0004638 |
| identifier_str_mv |
SAIDARRIAGA, Omar A. et al. An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections. PLoS Negl Trop Dis , v.10, n.4, e0004638, 12p, Apr. 2016. 1932-6203 10.1371/journal. pntd.0004638 |
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