Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

Detalhes bibliográficos
Autor(a) principal: Silva Neto, Aristeu
Data de Publicação: 2020
Outros Autores: Fonseca, Cristhiane Oliveira da, Silva, Luciana Maria, Coelho, Paulo Marcos Zech, Fortes-Dias, Consuelo Latorre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58740
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
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spelling Silva Neto, AristeuFonseca, Cristhiane Oliveira daSilva, Luciana MariaCoelho, Paulo Marcos ZechFortes-Dias, Consuelo Latorre2023-05-26T13:20:37Z2023-05-26T13:20:37Z2020SILVA NETO, Aristeu et al. Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts. Rev Soc Bras Med Trop., v. 53, e20200257, 2020. doi: 10.1590/0037-8682-0257-2020.0037-8682https://www.arca.fiocruz.br/handle/icict/5874010.1590/0037-8682-0257-2020engSociedade Brasileira De Medicina TropicalInteraction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocystsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, BrasilIntroduction: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria. Methods: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts. Results: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described. Conclusions: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.Biomphalaria tenagophilaBiomphalaria glabrataSchistosoma mansoniDigestive glandSaccular kidneyPrimary cell cultureinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALA_0257_2020.indd.pdfA_0257_2020.indd.pdfapplication/pdf1988070https://www.arca.fiocruz.br/bitstream/icict/58740/2/A_0257_2020.indd.pdf1403d56bf465f3fac1273f7a6a44be2eMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58740/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/587402023-05-26 10:20:39.264oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-26T13:20:39Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
spellingShingle Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
Silva Neto, Aristeu
Biomphalaria tenagophila
Biomphalaria glabrata
Schistosoma mansoni
Digestive gland
Saccular kidney
Primary cell culture
title_short Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title_full Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title_fullStr Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title_full_unstemmed Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title_sort Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
author Silva Neto, Aristeu
author_facet Silva Neto, Aristeu
Fonseca, Cristhiane Oliveira da
Silva, Luciana Maria
Coelho, Paulo Marcos Zech
Fortes-Dias, Consuelo Latorre
author_role author
author2 Fonseca, Cristhiane Oliveira da
Silva, Luciana Maria
Coelho, Paulo Marcos Zech
Fortes-Dias, Consuelo Latorre
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva Neto, Aristeu
Fonseca, Cristhiane Oliveira da
Silva, Luciana Maria
Coelho, Paulo Marcos Zech
Fortes-Dias, Consuelo Latorre
dc.subject.en.en_US.fl_str_mv Biomphalaria tenagophila
Biomphalaria glabrata
Schistosoma mansoni
Digestive gland
Saccular kidney
Primary cell culture
topic Biomphalaria tenagophila
Biomphalaria glabrata
Schistosoma mansoni
Digestive gland
Saccular kidney
Primary cell culture
description Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2023-05-26T13:20:37Z
dc.date.available.fl_str_mv 2023-05-26T13:20:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA NETO, Aristeu et al. Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts. Rev Soc Bras Med Trop., v. 53, e20200257, 2020. doi: 10.1590/0037-8682-0257-2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58740
dc.identifier.issn.en_US.fl_str_mv 0037-8682
dc.identifier.doi.none.fl_str_mv 10.1590/0037-8682-0257-2020
identifier_str_mv SILVA NETO, Aristeu et al. Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts. Rev Soc Bras Med Trop., v. 53, e20200257, 2020. doi: 10.1590/0037-8682-0257-2020.
0037-8682
10.1590/0037-8682-0257-2020
url https://www.arca.fiocruz.br/handle/icict/58740
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira De Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira De Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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