Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

Detalhes bibliográficos
Autor(a) principal: Silva-Neto,Aristeu
Data de Publicação: 2020
Outros Autores: Fonseca,Cristhiane Oliveira da, Silva,Luciana Maria, Coelho,Paulo Marcos Zech, Fortes-Dias,Consuelo Latorre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100374
Resumo: Abstract INTRODUCTION: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria. METHODS: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts. RESULTS: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described. CONCLUSIONS: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.
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spelling Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocystsBiomphalaria tenagophilaBiomphalaria glabrataSchistosoma mansoniDigestive glandSaccular kidneyPrimary cell cultureAbstract INTRODUCTION: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria. METHODS: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts. RESULTS: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described. CONCLUSIONS: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.Sociedade Brasileira de Medicina Tropical - SBMT2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100374Revista da Sociedade Brasileira de Medicina Tropical v.53 2020reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0257-2020info:eu-repo/semantics/openAccessSilva-Neto,AristeuFonseca,Cristhiane Oliveira daSilva,Luciana MariaCoelho,Paulo Marcos ZechFortes-Dias,Consuelo Latorreeng2020-12-11T00:00:00Zoai:scielo:S0037-86822020000100374Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2020-12-11T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
spellingShingle Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
Silva-Neto,Aristeu
Biomphalaria tenagophila
Biomphalaria glabrata
Schistosoma mansoni
Digestive gland
Saccular kidney
Primary cell culture
title_short Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title_full Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title_fullStr Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title_full_unstemmed Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
title_sort Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts
author Silva-Neto,Aristeu
author_facet Silva-Neto,Aristeu
Fonseca,Cristhiane Oliveira da
Silva,Luciana Maria
Coelho,Paulo Marcos Zech
Fortes-Dias,Consuelo Latorre
author_role author
author2 Fonseca,Cristhiane Oliveira da
Silva,Luciana Maria
Coelho,Paulo Marcos Zech
Fortes-Dias,Consuelo Latorre
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva-Neto,Aristeu
Fonseca,Cristhiane Oliveira da
Silva,Luciana Maria
Coelho,Paulo Marcos Zech
Fortes-Dias,Consuelo Latorre
dc.subject.por.fl_str_mv Biomphalaria tenagophila
Biomphalaria glabrata
Schistosoma mansoni
Digestive gland
Saccular kidney
Primary cell culture
topic Biomphalaria tenagophila
Biomphalaria glabrata
Schistosoma mansoni
Digestive gland
Saccular kidney
Primary cell culture
description Abstract INTRODUCTION: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria. METHODS: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts. RESULTS: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described. CONCLUSIONS: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100374
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100374
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-8682-0257-2020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.53 2020
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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