Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10172 |
Resumo: | UNICAMP. Instituto de Química. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil / UNICAMP. Instituto de Química. Instituto Nacional de Ciência e Tecnologia de Bioanalítica. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil. |
| id |
CRUZ_10f22aedfe077ad7e2439d094e931ea7 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/10172 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Hauser-Davis, Rachel AnnBastos, Frederico FreireDantas, Rafael FerreiraTobar, Santiago Alonso LeitãoBastos Neto, Jayme da CunhaBastos, Vera Lucia Freire da CunhaZiolli, Roberta LourençoArruda, Marco Aurélio Zezzi2015-05-04T16:34:33Z2015-05-04T16:34:33Z2014HAUSER-DAVIS, Rachel Ann et al. Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish. Ecotoxicology and Environmental Safety, v.103, p.24–28, 2014.0147-6513https://www.arca.fiocruz.br/handle/icict/1017210.1016/j.ecoenv.2014.01.015engScienceDirectBehaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fishinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUNICAMP. Instituto de Química. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil / UNICAMP. Instituto de Química. Instituto Nacional de Ciência e Tecnologia de Bioanalítica. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.UNICAMP. Instituto de Química. Instituto Nacional de Ciência e Tecnologia de Bioanalítica. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil.Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Biodiversidade Neotropical. Rio de Janeiro, RJ, Brasil.UNICAMP. Instituto de Química. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil / UNICAMP. Instituto de Química. Instituto Nacional de Ciência e Tecnologia de Bioanalítica. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil.The pacu (Piaractus mesopotamicus) is a hypoxia-tolerant neotropical fish species. There is little or no information in this species regarding biochemical adaptations to waters with different oxygen concentrations, such as the production of reactive oxygen species and antioxidant scavengers, which might be of interest in the study of antioxidant defense mechanisms. Metallothioneins (MT) have been widely applied as biomarkers for metal exposure in fish liver, and, recently, in bile. These metalloproteins, however, have also been reported as free radical scavengers, although studies in this regard are scarce in fish. In this context, normoxic and hypoxic controlled experiments were conducted with pacu specimens and MT levels were quantified in both liver and bile. Reduced glutathione (GSH) indicative of oxidative stress, and thiobarbituric acid reactive substances (TBARS), indicative of lipid peroxidation, were also determined in liver. The results demonstrate that hypoxic fish present significantly lower metallothionein levels in liver and bile and lower reduced glutathione levels in liver, whereas lipid peroxidation was not significantly different between hypoxic and normoxic fish. The results of the present study seem to suggest that metallothioneins may actively participate in redox regulation in hypoxic fish in both bile and liver. MT levels in these organs may be temporarily suppressed, supporting the notion that down-regulation of oxidant scavengers during the oxidative burst is important in defense signaling in these adapted organisms.MetallothioneinGSHTBARSFish bile and liverHypoxiaCiências BiológicasPeixesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10172/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALrafael_dantasetal_IOC_2014.pdfapplication/pdf315421https://www.arca.fiocruz.br/bitstream/icict/10172/2/rafael_dantasetal_IOC_2014.pdf0a3c17d1fbe1ca99bd346544efd0c2eeMD52TEXTrafael_dantasetal_IOC_2014.pdf.txtrafael_dantasetal_IOC_2014.pdf.txtExtracted texttext/plain29356https://www.arca.fiocruz.br/bitstream/icict/10172/3/rafael_dantasetal_IOC_2014.pdf.txt365d00bad79236f68b23bfc34fc00c8fMD53icict/101722022-06-24 12:18:57.45oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:18:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish |
| title |
Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish |
| spellingShingle |
Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish Hauser-Davis, Rachel Ann Metallothionein GSH TBARS Fish bile and liver Hypoxia Ciências Biológicas Peixes |
| title_short |
Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish |
| title_full |
Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish |
| title_fullStr |
Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish |
| title_full_unstemmed |
Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish |
| title_sort |
Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish |
| author |
Hauser-Davis, Rachel Ann |
| author_facet |
Hauser-Davis, Rachel Ann Bastos, Frederico Freire Dantas, Rafael Ferreira Tobar, Santiago Alonso Leitão Bastos Neto, Jayme da Cunha Bastos, Vera Lucia Freire da Cunha Ziolli, Roberta Lourenço Arruda, Marco Aurélio Zezzi |
| author_role |
author |
| author2 |
Bastos, Frederico Freire Dantas, Rafael Ferreira Tobar, Santiago Alonso Leitão Bastos Neto, Jayme da Cunha Bastos, Vera Lucia Freire da Cunha Ziolli, Roberta Lourenço Arruda, Marco Aurélio Zezzi |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Hauser-Davis, Rachel Ann Bastos, Frederico Freire Dantas, Rafael Ferreira Tobar, Santiago Alonso Leitão Bastos Neto, Jayme da Cunha Bastos, Vera Lucia Freire da Cunha Ziolli, Roberta Lourenço Arruda, Marco Aurélio Zezzi |
| dc.subject.en.pt_BR.fl_str_mv |
Metallothionein GSH TBARS Fish bile and liver Hypoxia |
| topic |
Metallothionein GSH TBARS Fish bile and liver Hypoxia Ciências Biológicas Peixes |
| dc.subject.decs.pt_BR.fl_str_mv |
Ciências Biológicas Peixes |
| description |
UNICAMP. Instituto de Química. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil / UNICAMP. Instituto de Química. Instituto Nacional de Ciência e Tecnologia de Bioanalítica. Grupo de Espectrometria, Preparo de amostras e Mecanização-GEPAM. Campinas, SP, Brasil. |
| publishDate |
2014 |
| dc.date.issued.fl_str_mv |
2014 |
| dc.date.accessioned.fl_str_mv |
2015-05-04T16:34:33Z |
| dc.date.available.fl_str_mv |
2015-05-04T16:34:33Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
HAUSER-DAVIS, Rachel Ann et al. Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish. Ecotoxicology and Environmental Safety, v.103, p.24–28, 2014. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/10172 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0147-6513 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.ecoenv.2014.01.015 |
| identifier_str_mv |
HAUSER-DAVIS, Rachel Ann et al. Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish. Ecotoxicology and Environmental Safety, v.103, p.24–28, 2014. 0147-6513 10.1016/j.ecoenv.2014.01.015 |
| url |
https://www.arca.fiocruz.br/handle/icict/10172 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
ScienceDirect |
| publisher.none.fl_str_mv |
ScienceDirect |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/10172/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/10172/2/rafael_dantasetal_IOC_2014.pdf https://www.arca.fiocruz.br/bitstream/icict/10172/3/rafael_dantasetal_IOC_2014.pdf.txt |
| bitstream.checksum.fl_str_mv |
7d48279ffeed55da8dfe2f8e81f3b81f 0a3c17d1fbe1ca99bd346544efd0c2ee 365d00bad79236f68b23bfc34fc00c8f |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1798324819193757696 |