Isolation and characterisation of metallothionein from the clam Ruditapes decussatus

Detalhes bibliográficos
Autor(a) principal: Moura, José J. G.
Data de Publicação: 2003
Outros Autores: Simes, Dina C., Bebianno, Maria João
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/1667
Resumo: Aquatic Toxicology 63 (2003) 307-318
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spelling Isolation and characterisation of metallothionein from the clam Ruditapes decussatusMetallothioneinClamRuditapes decussatusCdToxicityAquatic Toxicology 63 (2003) 307-318Metallothioneins (MT) were obtained after purification from metal-exposed clams (Ruditapes decussatus) using gelpermeation and ion-exchange chromatography. Four cadmium /metallothioneins (CdMTs) were resolved by ionexchange chromatography and they all had similar molecular weights, high cadmium content and an absorption spectra indicative of the presence of characteristic Cd /S aggregates. The NH2-terminal sequence suggests the presence of atleast two class I clam MT isoforms. For the other two putative clam CdMTs isolated, the results of the amino acid determination were inconclusive. One was slightly contaminated and the other one had a blocked NH2-terminal. These clam metalothioneins contain glycine, which seems to be a common feature of molluscan MT family and exhibited more similarity to oysters than to mussels. Further investigation on the inducibility of these isoforms will be necessary if clams are to be used as biomarkers of metal exposure.ElsevierRUNMoura, José J. G.Simes, Dina C.Bebianno, Maria João2008-09-25T11:38:09Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/1667eng0166-445Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:31:39Zoai:run.unl.pt:10362/1667Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:14:47.366969Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
title Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
spellingShingle Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
Moura, José J. G.
Metallothionein
Clam
Ruditapes decussatus
Cd
Toxicity
title_short Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
title_full Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
title_fullStr Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
title_full_unstemmed Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
title_sort Isolation and characterisation of metallothionein from the clam Ruditapes decussatus
author Moura, José J. G.
author_facet Moura, José J. G.
Simes, Dina C.
Bebianno, Maria João
author_role author
author2 Simes, Dina C.
Bebianno, Maria João
author2_role author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Moura, José J. G.
Simes, Dina C.
Bebianno, Maria João
dc.subject.por.fl_str_mv Metallothionein
Clam
Ruditapes decussatus
Cd
Toxicity
topic Metallothionein
Clam
Ruditapes decussatus
Cd
Toxicity
description Aquatic Toxicology 63 (2003) 307-318
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
2008-09-25T11:38:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/1667
url http://hdl.handle.net/10362/1667
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0166-445X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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