Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24926 |
Resumo: | Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). LGG and GRG were supported by scholarships from the Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP (2011/23819-0 and 2014/25856-8; 2013/00382-0, respectively). |
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Gardinassi, Luiz Gustavo AraujoVieira, Thiago Soares de SouzaSilva, Naila O. daGarcia, Gustavo RochaBorges, Valéria de MatosCampos, Roseane Nunes de SantanaAlmeida, Roque Pacheco deSantos, Isabel Kiney Ferreira de MirandaSaraiva, Elvira Maria2018-02-23T18:34:28Z2018-02-23T18:34:28Z2017GARDINASSI, L. G. A. et al.Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis. Parasites & Vectors, v. 10, p. 285, 2017.1756-3305https://www.arca.fiocruz.br/handle/icict/2492610.1186/s13071-017-2222-5Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). LGG and GRG were supported by scholarships from the Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP (2011/23819-0 and 2014/25856-8; 2013/00382-0, respectively).Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, BrazilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, BrazilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, BrazilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Sergipe. Hospital Universitário. Departamento de Medicina. Aracaju, SE, BrazilUniversidade Federal de Sergipe. Hospital Universitário. Departamento de Medicina. Aracaju, SE, BrazilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, BrazilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, BrazilInfections with parasites of the Leishmania donovani complex result in clinical outcomes that range from asymptomatic infection to severe and fatal visceral leishmaniasis (VL). Neutrophils are major players of the immune response against Leishmania, but their contribution to distinct states of infection is unknown. Gene expression data suggest the activation of the NETosis pathway during human visceral leishmaniasis. Thus, we conducted an exploratory study to evaluate NET-related molecules in retrospective sera from VL patients, asymptomatic individuals and uninfected endemic controls. Results: We demonstrate that VL patients and asymptomatic individuals exhibit differential regulation of molecules associated with neutrophil extracellular traps (NET). These differences were observed at the transcriptional level of genes encoding NET-associated proteins; in quantifications of cell free DNA and metalloproteinase 9; and in enzymatic activity of DNAse and elastase. Moreover, multivariate analysis resulted in class-specific signatures, and ROC curves demonstrate the ability of these molecules in discriminating asymptomatic infection from uninfected controls. Conclusion: Molecules that are associated with NETs are differentially regulated between distinct states of infection with L. infantum, suggesting that NETs might have distinct roles depending on the clinical status of infection. Although unlikely to be exclusive for VL, these signatures can be useful to better characterize asymptomatic infections in endemic regions of this disease.engBioMed CentralArmadilhas extracelulares de NeutrófilosNETosisLeishmania infantumLeishmaniose visceralInfecção assintomáticaNeutrophil extracellular trapsNETosisLeishmania infantumVisceral leishmaniasisAsymptomatic infectionMolecular signatures of neutrophil extracellular traps in human visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24926/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGardinassi LG Molecular signatures of neutrophil....pdfGardinassi LG Molecular signatures of 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| dc.title.pt_BR.fl_str_mv |
Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis |
| title |
Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis |
| spellingShingle |
Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis Gardinassi, Luiz Gustavo Araujo Armadilhas extracelulares de Neutrófilos NETosis Leishmania infantum Leishmaniose visceral Infecção assintomática Neutrophil extracellular traps NETosis Leishmania infantum Visceral leishmaniasis Asymptomatic infection |
| title_short |
Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis |
| title_full |
Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis |
| title_fullStr |
Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis |
| title_full_unstemmed |
Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis |
| title_sort |
Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis |
| author |
Gardinassi, Luiz Gustavo Araujo |
| author_facet |
Gardinassi, Luiz Gustavo Araujo Vieira, Thiago Soares de Souza Silva, Naila O. da Garcia, Gustavo Rocha Borges, Valéria de Matos Campos, Roseane Nunes de Santana Almeida, Roque Pacheco de Santos, Isabel Kiney Ferreira de Miranda Saraiva, Elvira Maria |
| author_role |
author |
| author2 |
Vieira, Thiago Soares de Souza Silva, Naila O. da Garcia, Gustavo Rocha Borges, Valéria de Matos Campos, Roseane Nunes de Santana Almeida, Roque Pacheco de Santos, Isabel Kiney Ferreira de Miranda Saraiva, Elvira Maria |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gardinassi, Luiz Gustavo Araujo Vieira, Thiago Soares de Souza Silva, Naila O. da Garcia, Gustavo Rocha Borges, Valéria de Matos Campos, Roseane Nunes de Santana Almeida, Roque Pacheco de Santos, Isabel Kiney Ferreira de Miranda Saraiva, Elvira Maria |
| dc.subject.other.pt_BR.fl_str_mv |
Armadilhas extracelulares de Neutrófilos NETosis Leishmania infantum Leishmaniose visceral Infecção assintomática |
| topic |
Armadilhas extracelulares de Neutrófilos NETosis Leishmania infantum Leishmaniose visceral Infecção assintomática Neutrophil extracellular traps NETosis Leishmania infantum Visceral leishmaniasis Asymptomatic infection |
| dc.subject.en.pt_BR.fl_str_mv |
Neutrophil extracellular traps NETosis Leishmania infantum Visceral leishmaniasis Asymptomatic infection |
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Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). LGG and GRG were supported by scholarships from the Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP (2011/23819-0 and 2014/25856-8; 2013/00382-0, respectively). |
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2017 |
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2017 |
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2018-02-23T18:34:28Z |
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2018-02-23T18:34:28Z |
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GARDINASSI, L. G. A. et al.Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis. Parasites & Vectors, v. 10, p. 285, 2017. |
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https://www.arca.fiocruz.br/handle/icict/24926 |
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1756-3305 |
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10.1186/s13071-017-2222-5 |
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GARDINASSI, L. G. A. et al.Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis. Parasites & Vectors, v. 10, p. 285, 2017. 1756-3305 10.1186/s13071-017-2222-5 |
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