PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells

Detalhes bibliográficos
Autor(a) principal: Lemos, Ana Emília Goulart
Data de Publicação: 2016
Outros Autores: Ferreira, Luciana Bueno, Batoreu, Nadia Maria, Freitas, Paula Priscilla de, Bonamino, Martin Hernan, Gimba, Etel Rodrigues Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13673
Resumo: Fundação Oswaldo Cruz, Bio-Manguinhos, Rio de Janeiro, Brazil
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spelling Lemos, Ana Emília GoulartFerreira, Luciana BuenoBatoreu, Nadia MariaFreitas, Paula Priscilla deBonamino, Martin HernanGimba, Etel Rodrigues Pereira2016-04-11T17:08:09Z2016-04-11T17:08:09Z2016LEMOS, Ana Emília Goulart et al. PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells.Tumor Biology, p. 1-10, mar. 2016.1010-4283https://www.arca.fiocruz.br/handle/icict/136731423-0380engSpringerExpressão GênicaNeoplasias da PróstataPCA3Long noncoding RNAGene expressionCancer-related genesProstatic NeoplasmsExpressão GênicaProstatic NeoplasmsPCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz, Bio-Manguinhos, Rio de Janeiro, BrazilInstituto de Patologia Molecular e Imunologia da Universidade do Porto, Porto, PortugalFundação Centro Universitário Estadual da Zona Oeste, Rio de Janeiro, BrazilInstituto Nacional de Câncer José Alencar Gomes da Silva, Coordenação de Pesquisa, Rio de Janeiro, BrazilFundação Oswaldo Cruz, Vice-presidência de Pesquisa e Laboratórios de Referência, Rio de Janeiro, BrazilUniversidade Federal Fluminense, Instituto de Humanidades e Sáude, Departamento de Ciências da Natureza, Rio de Janeiro, BrazilProstate cancer antigen 3 (PCA3)is a prostate-specific long noncoding RNA (lncRNA) involved in the control of prostate cancer (PCa) cell survival, through modulating androgen receptor (AR) signaling.To furthercomprehendthemechanisms by which PCA3 modulates LNCaP cell survival, we characterized the expression patterns of several cancer-related genes, including those involved in epithelial-mesenchymal transition (EMT) and AR cofactors in response to PCA3 silencing. We also aimed to develop a strategyto stably silence PCA3. SmallinterferingRNA (siRNA) or short hairpinRNA (shRNA) was usedto knock down PCA3inLNCaP cells.The expression of 84cancer-related genes, as well as those coding for AR cofactors and EMT markers, was analyzed by quantitative real-time PCR (qRT-PCR). LNCaPPCA3 silenced cells differentially expressed 16 of the 84 cancer genes tested, mainly those involved in gene expression control and cell signaling. PCA3 knockdown also induced the upregulation of severaltranscripts coding for AR cofactors and modulated the expression of EMT markers. LNCaP cells transduced with lentivirus vectors carrying an shRNA sequence targeting PCA3 stably downregulated PCA3 expression, causing a significant drop (60 %) in the proportion of LNCaP cells expressing the transgene. In conclusion, our data provide evidence that PCA3 silencing modulates the expression of key cancer-related genes, including those coding for AR cofactors and EMT markers. Transducing LNCaP cells with an shRNA sequence targeting PCA3 led to loss of viability of the cells, supporting the proposal of PCA3 knockdown as a putativetherapeutic approachtoinhibit PCa growth.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALLemos-et-al-2016.pdfLemos-et-al-2016.pdfapplication/pdf1468872https://www.arca.fiocruz.br/bitstream/icict/13673/2/Lemos-et-al-2016.pdf06c44b6803fda29fc48fc1f33022ab35MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13673/3/license.txt5a560609d32a3863062d77ff32785d58MD53TEXTLemos-et-al-2016.pdf.txtLemos-et-al-2016.pdf.txtExtracted texttext/plain57104https://www.arca.fiocruz.br/bitstream/icict/13673/4/Lemos-et-al-2016.pdf.txtdf76b177dd7d98ea5645ad26ed658299MD54icict/136732018-04-02 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dc.title.pt_BR.fl_str_mv PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells
title PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells
spellingShingle PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells
Lemos, Ana Emília Goulart
Expressão Gênica
Neoplasias da Próstata
PCA3
Long noncoding RNA
Gene expression
Cancer-related genes
Prostatic Neoplasms
Expressão Gênica
Prostatic Neoplasms
title_short PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells
title_full PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells
title_fullStr PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells
title_full_unstemmed PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells
title_sort PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells
author Lemos, Ana Emília Goulart
author_facet Lemos, Ana Emília Goulart
Ferreira, Luciana Bueno
Batoreu, Nadia Maria
Freitas, Paula Priscilla de
Bonamino, Martin Hernan
Gimba, Etel Rodrigues Pereira
author_role author
author2 Ferreira, Luciana Bueno
Batoreu, Nadia Maria
Freitas, Paula Priscilla de
Bonamino, Martin Hernan
Gimba, Etel Rodrigues Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lemos, Ana Emília Goulart
Ferreira, Luciana Bueno
Batoreu, Nadia Maria
Freitas, Paula Priscilla de
Bonamino, Martin Hernan
Gimba, Etel Rodrigues Pereira
dc.subject.other.pt_BR.fl_str_mv Expressão Gênica
Neoplasias da Próstata
topic Expressão Gênica
Neoplasias da Próstata
PCA3
Long noncoding RNA
Gene expression
Cancer-related genes
Prostatic Neoplasms
Expressão Gênica
Prostatic Neoplasms
dc.subject.en.pt_BR.fl_str_mv PCA3
Long noncoding RNA
Gene expression
Cancer-related genes
Prostatic Neoplasms
dc.subject.decs.pt_BR.fl_str_mv Expressão Gênica
Prostatic Neoplasms
description Fundação Oswaldo Cruz, Bio-Manguinhos, Rio de Janeiro, Brazil
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-04-11T17:08:09Z
dc.date.available.fl_str_mv 2016-04-11T17:08:09Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LEMOS, Ana Emília Goulart et al. PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells.Tumor Biology, p. 1-10, mar. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13673
dc.identifier.issn.none.fl_str_mv 1010-4283
dc.identifier.eissn.none.fl_str_mv 1423-0380
identifier_str_mv LEMOS, Ana Emília Goulart et al. PCA3 long noncoding RNA modulates the expression of key cancer-related genes in LNCaP prostate cancer cells.Tumor Biology, p. 1-10, mar. 2016.
1010-4283
1423-0380
url https://www.arca.fiocruz.br/handle/icict/13673
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/13673/2/Lemos-et-al-2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/13673/3/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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