Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11608 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. |
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Bentes, Gentil ArthurLanzarini, Natália MariaLima, Lyana Rodrigues PintoManso, Pedro Paulo de AbreuSilva, Alexandre dos Santos daMouta Junior, Sergio da Silva eGuimarães, Juliana RodriguesMoraes, Marcia Terezinha Baroni deMachado, Marcelo PelajoPinto, Marcelo Alves2015-08-19T13:49:40Z2015-08-19T13:49:40Z2015BENTES, Gentil Arthur; et al. Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections. Mem Inst Oswaldo Cruz, v.110, n.4, p.577-579, June 2015.1678-8060https://www.arca.fiocruz.br/handle/icict/1160810.1590/0074-02760140457engFundação Oswaldo CruzUsing immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ,Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ,Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.Immunoglobulin YHepatitis A virusIndirect immunofluorescenceVírus da Hepatite AImunoglobulina Yinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11608/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALgentil_bentes_etal_IOC_2015.pdfapplication/pdf573420https://www.arca.fiocruz.br/bitstream/icict/11608/2/gentil_bentes_etal_IOC_2015.pdf9fb606d9da99892ab59cedf804d67dedMD52TEXTgentil_bentes_etal_IOC_2015.pdf.txtgentil_bentes_etal_IOC_2015.pdf.txtExtracted texttext/plain14478https://www.arca.fiocruz.br/bitstream/icict/11608/3/gentil_bentes_etal_IOC_2015.pdf.txtd2de01ed27d2575355c7ed77231a83e4MD53icict/116082022-06-24 12:59:51.072oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:59:51Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| spellingShingle |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections Bentes, Gentil Arthur Immunoglobulin Y Hepatitis A virus Indirect immunofluorescence Vírus da Hepatite A Imunoglobulina Y |
| title_short |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_full |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_fullStr |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_full_unstemmed |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_sort |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| author |
Bentes, Gentil Arthur |
| author_facet |
Bentes, Gentil Arthur Lanzarini, Natália Maria Lima, Lyana Rodrigues Pinto Manso, Pedro Paulo de Abreu Silva, Alexandre dos Santos da Mouta Junior, Sergio da Silva e Guimarães, Juliana Rodrigues Moraes, Marcia Terezinha Baroni de Machado, Marcelo Pelajo Pinto, Marcelo Alves |
| author_role |
author |
| author2 |
Lanzarini, Natália Maria Lima, Lyana Rodrigues Pinto Manso, Pedro Paulo de Abreu Silva, Alexandre dos Santos da Mouta Junior, Sergio da Silva e Guimarães, Juliana Rodrigues Moraes, Marcia Terezinha Baroni de Machado, Marcelo Pelajo Pinto, Marcelo Alves |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bentes, Gentil Arthur Lanzarini, Natália Maria Lima, Lyana Rodrigues Pinto Manso, Pedro Paulo de Abreu Silva, Alexandre dos Santos da Mouta Junior, Sergio da Silva e Guimarães, Juliana Rodrigues Moraes, Marcia Terezinha Baroni de Machado, Marcelo Pelajo Pinto, Marcelo Alves |
| dc.subject.en.pt_BR.fl_str_mv |
Immunoglobulin Y Hepatitis A virus Indirect immunofluorescence |
| topic |
Immunoglobulin Y Hepatitis A virus Indirect immunofluorescence Vírus da Hepatite A Imunoglobulina Y |
| dc.subject.decs.pt_BR.fl_str_mv |
Vírus da Hepatite A Imunoglobulina Y |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. |
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2015 |
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2015-08-19T13:49:40Z |
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2015-08-19T13:49:40Z |
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2015 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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BENTES, Gentil Arthur; et al. Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections. Mem Inst Oswaldo Cruz, v.110, n.4, p.577-579, June 2015. |
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https://www.arca.fiocruz.br/handle/icict/11608 |
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1678-8060 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/0074-02760140457 |
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BENTES, Gentil Arthur; et al. Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections. Mem Inst Oswaldo Cruz, v.110, n.4, p.577-579, June 2015. 1678-8060 10.1590/0074-02760140457 |
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