Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections

Detalhes bibliográficos
Autor(a) principal: Bentes,Gentil Arthur
Data de Publicação: 2015
Outros Autores: Lanzarini,Natália Maria, Lima,Lyana Rodrigues Pinto, Manso,Pedro Paulo de Abreu, Silva,Alexandre dos Santos da, Mouta Junior,Sergio da Silva e, Guimarães,Juliana Rodrigues, Moraes,Marcia Terezinha Baroni de, Pelajo-Machado,Marcelo, Pinto,Marcelo Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577
Resumo: An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.
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spelling Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sectionsimmunoglobulin Yhepatitis A virusindirect immunofluorescenceAn increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.Instituto Oswaldo Cruz, Ministério da Saúde2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577Memórias do Instituto Oswaldo Cruz v.110 n.4 2015reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760140457info:eu-repo/semantics/openAccessBentes,Gentil ArthurLanzarini,Natália MariaLima,Lyana Rodrigues PintoManso,Pedro Paulo de AbreuSilva,Alexandre dos Santos daMouta Junior,Sergio da Silva eGuimarães,Juliana RodriguesMoraes,Marcia Terezinha Baroni dePelajo-Machado,MarceloPinto,Marcelo Alveseng2020-04-25T17:52:14Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:45.687Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
title Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
spellingShingle Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
Bentes,Gentil Arthur
immunoglobulin Y
hepatitis A virus
indirect immunofluorescence
title_short Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
title_full Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
title_fullStr Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
title_full_unstemmed Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
title_sort Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
author Bentes,Gentil Arthur
author_facet Bentes,Gentil Arthur
Lanzarini,Natália Maria
Lima,Lyana Rodrigues Pinto
Manso,Pedro Paulo de Abreu
Silva,Alexandre dos Santos da
Mouta Junior,Sergio da Silva e
Guimarães,Juliana Rodrigues
Moraes,Marcia Terezinha Baroni de
Pelajo-Machado,Marcelo
Pinto,Marcelo Alves
author_role author
author2 Lanzarini,Natália Maria
Lima,Lyana Rodrigues Pinto
Manso,Pedro Paulo de Abreu
Silva,Alexandre dos Santos da
Mouta Junior,Sergio da Silva e
Guimarães,Juliana Rodrigues
Moraes,Marcia Terezinha Baroni de
Pelajo-Machado,Marcelo
Pinto,Marcelo Alves
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bentes,Gentil Arthur
Lanzarini,Natália Maria
Lima,Lyana Rodrigues Pinto
Manso,Pedro Paulo de Abreu
Silva,Alexandre dos Santos da
Mouta Junior,Sergio da Silva e
Guimarães,Juliana Rodrigues
Moraes,Marcia Terezinha Baroni de
Pelajo-Machado,Marcelo
Pinto,Marcelo Alves
dc.subject.por.fl_str_mv immunoglobulin Y
hepatitis A virus
indirect immunofluorescence
topic immunoglobulin Y
hepatitis A virus
indirect immunofluorescence
dc.description.none.fl_txt_mv An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.
description An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-02760140457
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.110 n.4 2015
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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