Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577 |
Resumo: | An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies. |
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Memórias do Instituto Oswaldo Cruz |
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Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sectionsimmunoglobulin Yhepatitis A virusindirect immunofluorescenceAn increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.Instituto Oswaldo Cruz, Ministério da Saúde2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577Memórias do Instituto Oswaldo Cruz v.110 n.4 2015reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760140457info:eu-repo/semantics/openAccessBentes,Gentil ArthurLanzarini,Natália MariaLima,Lyana Rodrigues PintoManso,Pedro Paulo de AbreuSilva,Alexandre dos Santos daMouta Junior,Sergio da Silva eGuimarães,Juliana RodriguesMoraes,Marcia Terezinha Baroni dePelajo-Machado,MarceloPinto,Marcelo Alveseng2020-04-25T17:52:14Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:45.687Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
title |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
spellingShingle |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections Bentes,Gentil Arthur immunoglobulin Y hepatitis A virus indirect immunofluorescence |
title_short |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
title_full |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
title_fullStr |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
title_full_unstemmed |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
title_sort |
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
author |
Bentes,Gentil Arthur |
author_facet |
Bentes,Gentil Arthur Lanzarini,Natália Maria Lima,Lyana Rodrigues Pinto Manso,Pedro Paulo de Abreu Silva,Alexandre dos Santos da Mouta Junior,Sergio da Silva e Guimarães,Juliana Rodrigues Moraes,Marcia Terezinha Baroni de Pelajo-Machado,Marcelo Pinto,Marcelo Alves |
author_role |
author |
author2 |
Lanzarini,Natália Maria Lima,Lyana Rodrigues Pinto Manso,Pedro Paulo de Abreu Silva,Alexandre dos Santos da Mouta Junior,Sergio da Silva e Guimarães,Juliana Rodrigues Moraes,Marcia Terezinha Baroni de Pelajo-Machado,Marcelo Pinto,Marcelo Alves |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bentes,Gentil Arthur Lanzarini,Natália Maria Lima,Lyana Rodrigues Pinto Manso,Pedro Paulo de Abreu Silva,Alexandre dos Santos da Mouta Junior,Sergio da Silva e Guimarães,Juliana Rodrigues Moraes,Marcia Terezinha Baroni de Pelajo-Machado,Marcelo Pinto,Marcelo Alves |
dc.subject.por.fl_str_mv |
immunoglobulin Y hepatitis A virus indirect immunofluorescence |
topic |
immunoglobulin Y hepatitis A virus indirect immunofluorescence |
dc.description.none.fl_txt_mv |
An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies. |
description |
An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-02760140457 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.110 n.4 2015 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937719326801920 |