Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Barros, Roberto Rudge de Moraes
Data de Publicação: 2012
Outros Autores: Souza, Marjorie Mendes Marini e, Antônio, Cristiane Regina, Cortez, Danielle Rodrigues, Miyake, Andréa Midory, Lima, Fabio Mitsuo, Ruiz, Jerônimo Conceição, Bartholomeu, Daniella Castanheira, Chiurillo, Miguel Angel, Ramirez, José Luis, Silveira, José Franco da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7800
Resumo: Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.
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spelling Barros, Roberto Rudge de MoraesSouza, Marjorie Mendes Marini eAntônio, Cristiane ReginaCortez, Danielle RodriguesMiyake, Andréa MidoryLima, Fabio MitsuoRuiz, Jerônimo ConceiçãoBartholomeu, Daniella CastanheiraChiurillo, Miguel AngelRamirez, José LuisSilveira, José Franco da2014-06-05T14:21:37Z2014-06-05T14:21:37Z2012BARROS, Roberto Rudge de Moraes et al. Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi. BMC Genomics, v. 13, p. 1-16, 2012.1471-2164https://www.arca.fiocruz.br/handle/icict/780010.1186/1471-2164-13-229engBioMed CentralAnatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.Universidad Centroccidental Lisandro Alvarado. Decanato de Ciencias de la Salud. Barquisimeto, Venezuela.Fundación Instituto de Estudios Avanzados. Caracas, Venezuela.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Background: The subtelomeres of many protozoa are highly enriched in genes with roles in niche adaptation. T. cruzi trypomastigotes express surface proteins from Trans-Sialidase (TS) and Dispersed Gene Family-1 (DGF-1) superfamilies which are implicated in host cell invasion. Single populations of T. cruzi may express different antigenic forms of TSs. Analysis of TS genes located at the telomeres suggests that chromosome ends could have been the sites where new TS variants were generated. The aim of this study is to characterize telomeric and subtelomeric regions of T. cruzi available in TriTrypDB and connect the sequences of telomeres to T. cruzi working draft sequence. Results: We first identified contigs carrying the telomeric repeat (TTAGGG). Of 49 contigs identified, 45 have telomeric repeats at one end, whereas in four contigs the repeats are located internally. All contigs display a conserved telomeric junction sequence adjacent to the hexamer repeats which represents a signature of T. cruzi chromosome ends. We found that 40 telomeric contigs are located on T. cruzi chromosome-sized scaffolds. In addition, we were able to map several telomeric ends to the chromosomal bands separated by pulsed-field gel electrophoresis. The subtelomeric sequence structure varies widely, mainly as a result of large differences in the relative abundance and organization of genes encoding surface proteins (TS and DGF-1), retrotransposon hot spot genes (RHS), retrotransposon elements, RNA-helicase and N-acetyltransferase genes. While the subtelomeric regions are enriched in pseudogenes, they also contain complete gene sequences matching both known and unknown expressed genes, indicating that these regions do not consist of nonfunctional DNA but are instead functional parts of the expressed genome. The size of the subtelomeric regions varies from 5 to 182 kb; the smaller of these regions could have been generated by a recent chromosome breakage and telomere healing event. Conclusions: The lack of synteny in the subtelomeric regions suggests that genes located in these regions are subject to recombination, which increases their variability, even among homologous chromosomes. The presence of typical subtelomeric genes can increase the chance of homologous recombination mechanisms or microhomology-mediated end joining, which may use these regions for the pairing and recombination of free ends.T. cruzi trypomastigotesTrans-Sialidaseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAnatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi.pdfAnatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi.pdfapplication/pdf3870315https://www.arca.fiocruz.br/bitstream/icict/7800/1/Anatomy%20and%20evolution%20of%20telomeric%20and%20subtelomeric%20regions%20in%20the%20human%20protozoan%20parasite%20Trypanosoma%20cruzi.pdf69089c03d0637a9606794bdffb7b9c78MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7800/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTAnatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi.pdf.txtAnatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi.pdf.txtExtracted texttext/plain16https://www.arca.fiocruz.br/bitstream/icict/7800/5/Anatomy%20and%20evolution%20of%20telomeric%20and%20subtelomeric%20regions%20in%20the%20human%20protozoan%20parasite%20Trypanosoma%20cruzi.pdf.txt24d68299ae13852334a122ea657bbaaaMD55THUMBNAILAnatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi.pdf.jpgAnatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi.pdf.jpgGenerated Thumbnailimage/jpeg1820https://www.arca.fiocruz.br/bitstream/icict/7800/4/Anatomy%20and%20evolution%20of%20telomeric%20and%20subtelomeric%20regions%20in%20the%20human%20protozoan%20parasite%20Trypanosoma%20cruzi.pdf.jpg03bb2d81b40c530ec99663081b2480d9MD54icict/78002018-06-21 18:28:36.197oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-06-21T21:28:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi
title Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi
spellingShingle Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi
Barros, Roberto Rudge de Moraes
T. cruzi trypomastigotes
Trans-Sialidase
title_short Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi
title_full Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi
title_fullStr Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi
title_full_unstemmed Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi
title_sort Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi
author Barros, Roberto Rudge de Moraes
author_facet Barros, Roberto Rudge de Moraes
Souza, Marjorie Mendes Marini e
Antônio, Cristiane Regina
Cortez, Danielle Rodrigues
Miyake, Andréa Midory
Lima, Fabio Mitsuo
Ruiz, Jerônimo Conceição
Bartholomeu, Daniella Castanheira
Chiurillo, Miguel Angel
Ramirez, José Luis
Silveira, José Franco da
author_role author
author2 Souza, Marjorie Mendes Marini e
Antônio, Cristiane Regina
Cortez, Danielle Rodrigues
Miyake, Andréa Midory
Lima, Fabio Mitsuo
Ruiz, Jerônimo Conceição
Bartholomeu, Daniella Castanheira
Chiurillo, Miguel Angel
Ramirez, José Luis
Silveira, José Franco da
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barros, Roberto Rudge de Moraes
Souza, Marjorie Mendes Marini e
Antônio, Cristiane Regina
Cortez, Danielle Rodrigues
Miyake, Andréa Midory
Lima, Fabio Mitsuo
Ruiz, Jerônimo Conceição
Bartholomeu, Daniella Castanheira
Chiurillo, Miguel Angel
Ramirez, José Luis
Silveira, José Franco da
dc.subject.en.pt_BR.fl_str_mv T. cruzi trypomastigotes
Trans-Sialidase
topic T. cruzi trypomastigotes
Trans-Sialidase
description Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-06-05T14:21:37Z
dc.date.available.fl_str_mv 2014-06-05T14:21:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARROS, Roberto Rudge de Moraes et al. Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi. BMC Genomics, v. 13, p. 1-16, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7800
dc.identifier.issn.none.fl_str_mv 1471-2164
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2164-13-229
identifier_str_mv BARROS, Roberto Rudge de Moraes et al. Anatomy and evolution of telomeric and subtelomeric regions in the human protozoan parasite Trypanosoma cruzi. BMC Genomics, v. 13, p. 1-16, 2012.
1471-2164
10.1186/1471-2164-13-229
url https://www.arca.fiocruz.br/handle/icict/7800
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7800/1/Anatomy%20and%20evolution%20of%20telomeric%20and%20subtelomeric%20regions%20in%20the%20human%20protozoan%20parasite%20Trypanosoma%20cruzi.pdf
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