Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene

Detalhes bibliográficos
Autor(a) principal: Santos, Eduardo L.
Data de Publicação: 2021
Outros Autores: Freitas, Priscilla R., Araujo, Ana Carolina J., Almeida, Ray S., Tintino, Saulo R., Paulo, Cicera Laura R., Ribeiro Filho, Jaime, Silva, Ana Cristina A., Silva, Luiz E, Amaral, Wanderlei do, Deschampse, Cícero, Siqueira Junior, Jose Pinto, Barbosa Filho, Jose Maria, Sousa, Gabriela Ribeiro de, Coutinho, Henrique D.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/49212
Resumo: a Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, Brazil b Gon¸calo Moniz Institute (IGM), Oswaldo Cruz Foundation (FIOCRUZ), Salvador, BA, Brazil c Maurício de Nassau College -UNINASSAU, Petrolina, PE, Brazil d Post graduate Program in Sustainable Territorial Development, Federal University of Paran a, UFPR, Matinhos, Brazil e Post graduate Program in Agronomy, Federal University of Paran a - UFPR, Curitiba, Brazil f Departmento of Molecular Biology, Federal University of Paraiba-UFPB, Jo~ao Pessoa 58051-900, PB, Brazil g Departmento of Pharmacy, Federal University of Paraiba-UFPB, Jo~ao Pessoa 58051-900, PB, Brazil
id CRUZ_120c395ba2c3b5314111a66723f76351
oai_identifier_str oai:www.arca.fiocruz.br:icict/49212
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Santos, Eduardo L.Freitas, Priscilla R.Araujo, Ana Carolina J.Almeida, Ray S.Tintino, Saulo R.Paulo, Cicera Laura R.Ribeiro Filho, JaimeSilva, Ana Cristina A.Silva, Luiz EAmaral, Wanderlei doDeschampse, CíceroSiqueira Junior, Jose PintoBarbosa Filho, Jose MariaSousa, Gabriela Ribeiro deCoutinho, Henrique D.M.2021-09-30T11:56:28Z2021-09-30T11:56:28Z2021SANTOS, Eduardo L. et al. Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene. South African Journal of Botany, 2021.0254-6299https://www.arca.fiocruz.br/handle/icict/4921210.1016/j.sajb.2021.07.046a Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, Brazil b Gon¸calo Moniz Institute (IGM), Oswaldo Cruz Foundation (FIOCRUZ), Salvador, BA, Brazil c Maurício de Nassau College -UNINASSAU, Petrolina, PE, Brazil d Post graduate Program in Sustainable Territorial Development, Federal University of Paran a, UFPR, Matinhos, Brazil e Post graduate Program in Agronomy, Federal University of Paran a - UFPR, Curitiba, Brazil f Departmento of Molecular Biology, Federal University of Paraiba-UFPB, Jo~ao Pessoa 58051-900, PB, Brazil g Departmento of Pharmacy, Federal University of Paraiba-UFPB, Jo~ao Pessoa 58051-900, PB, Brazil"Múltipla ver em Notas"The traditional knowledge associated with the use of medicinal plants has significantly contributed to drug development research. In this context, Aloysia gratissima, commonly known as bee-brush or white-brush, has been widely used in Brazilian folk medicine as an analgesic and antimicrobial species. Phytochemical analysis of A. gratissima essential oil has identified sesquiterpene b-caryophyllene (17.3%) as a major component with significant antibacterial activity. Thus, this study aimed to characterize the antibacterial effects of the essential oil obtained from A. gratissima (EOAG) and its major compound b-caryophyllene, as well as to evaluate their potential to act as efflux pumps inhibitors in association with conventional antibiotics. The phytochemical characterization of EOAG was performed by gas chromatography coupled to mass spectrometry (GC-MS), while the antibacterial activity against the strains Pseudomonas aeruginosa 24, Staphylococcus aureus 10, and Escherichia coli 06 was assessed using the broth microdilution method. The antibiotic potentiating activity was investigated by analyzing the ability of the natural products to decrease the minimum inhibitory concentration (MIC) of norfloxacin and ciprofloxacin against S. aureus strains 1199B and K2068. A reduction in the MIC of ethidium bromide in the presence of essential oil components was interpreted as efflux pump inhibition. The GC-MS analysis identified 30 compounds, including b-caryophyllene as the major component. The antibacterial activity analysis demonstrated that both EOAG and b-caryophyllene both EOAG and b-caryophyllene presented antibacterial effects against S. aureus 10 (MIC = 32 mg/mL), in addition to potentiating the activity of norfloxacin against [S. aureus 10], [P. aeruginosa 24], and [E. coli 06]. The isolated compound also reversed the degree of antibiotic resistance observed in strains carrying efflux pumps. In conclusion, the essential oil of A. gratissima and b-caryophyllene have the potential to increase the effectiveness of antibiotics and as such can be used in the development of new therapies against bacterial resistance.engElsevierPseudomonas aeruginosaEspectrometria de MassasResistência bacterianaBombasCiprofloxacinaAloysia gratissimab-caryophylleneBacterial resistanceEfflux pump inhibitionPhytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophylleneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/49212/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALSantos, Eduardo L Phytochemical characterization....pdfSantos, Eduardo L Phytochemical characterization....pdfapplication/pdf666866https://www.arca.fiocruz.br/bitstream/icict/49212/2/Santos%2c%20Eduardo%20L%20Phytochemical%20characterization....pdf0b6526809d9515d60e87bc887229ad52MD52icict/492122023-03-15 14:34:27.749oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:27Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene
title Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene
spellingShingle Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene
Santos, Eduardo L.
Pseudomonas aeruginosa
Espectrometria de Massas
Resistência bacteriana
Bombas
Ciprofloxacina
Aloysia gratissima
b-caryophyllene
Bacterial resistance
Efflux pump inhibition
title_short Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene
title_full Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene
title_fullStr Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene
title_full_unstemmed Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene
title_sort Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene
author Santos, Eduardo L.
author_facet Santos, Eduardo L.
Freitas, Priscilla R.
Araujo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Ribeiro Filho, Jaime
Silva, Ana Cristina A.
Silva, Luiz E
Amaral, Wanderlei do
Deschampse, Cícero
Siqueira Junior, Jose Pinto
Barbosa Filho, Jose Maria
Sousa, Gabriela Ribeiro de
Coutinho, Henrique D.M.
author_role author
author2 Freitas, Priscilla R.
Araujo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Ribeiro Filho, Jaime
Silva, Ana Cristina A.
Silva, Luiz E
Amaral, Wanderlei do
Deschampse, Cícero
Siqueira Junior, Jose Pinto
Barbosa Filho, Jose Maria
Sousa, Gabriela Ribeiro de
Coutinho, Henrique D.M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Eduardo L.
Freitas, Priscilla R.
Araujo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Ribeiro Filho, Jaime
Silva, Ana Cristina A.
Silva, Luiz E
Amaral, Wanderlei do
Deschampse, Cícero
Siqueira Junior, Jose Pinto
Barbosa Filho, Jose Maria
Sousa, Gabriela Ribeiro de
Coutinho, Henrique D.M.
dc.subject.other.pt_BR.fl_str_mv Pseudomonas aeruginosa
Espectrometria de Massas
Resistência bacteriana
Bombas
Ciprofloxacina
topic Pseudomonas aeruginosa
Espectrometria de Massas
Resistência bacteriana
Bombas
Ciprofloxacina
Aloysia gratissima
b-caryophyllene
Bacterial resistance
Efflux pump inhibition
dc.subject.en.pt_BR.fl_str_mv Aloysia gratissima
b-caryophyllene
Bacterial resistance
Efflux pump inhibition
description a Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, Brazil b Gon¸calo Moniz Institute (IGM), Oswaldo Cruz Foundation (FIOCRUZ), Salvador, BA, Brazil c Maurício de Nassau College -UNINASSAU, Petrolina, PE, Brazil d Post graduate Program in Sustainable Territorial Development, Federal University of Paran a, UFPR, Matinhos, Brazil e Post graduate Program in Agronomy, Federal University of Paran a - UFPR, Curitiba, Brazil f Departmento of Molecular Biology, Federal University of Paraiba-UFPB, Jo~ao Pessoa 58051-900, PB, Brazil g Departmento of Pharmacy, Federal University of Paraiba-UFPB, Jo~ao Pessoa 58051-900, PB, Brazil
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-09-30T11:56:28Z
dc.date.available.fl_str_mv 2021-09-30T11:56:28Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Eduardo L. et al. Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene. South African Journal of Botany, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49212
dc.identifier.issn.pt_BR.fl_str_mv 0254-6299
dc.identifier.doi.none.fl_str_mv 10.1016/j.sajb.2021.07.046
identifier_str_mv SANTOS, Eduardo L. et al. Phytochemical characterization and antibiotic potentiating effects of the essential oil of Aloysia gratissima (Gillies & Hook.) and beta-caryophyllene. South African Journal of Botany, 2021.
0254-6299
10.1016/j.sajb.2021.07.046
url https://www.arca.fiocruz.br/handle/icict/49212
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/49212/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/49212/2/Santos%2c%20Eduardo%20L%20Phytochemical%20characterization....pdf
bitstream.checksum.fl_str_mv 36b51ef91c52b5338d9d29ba0cc807bc
0b6526809d9515d60e87bc887229ad52
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324746503323648

Registros relacionados