Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation

Detalhes bibliográficos
Autor(a) principal: Duarte, Ângelo
Data de Publicação: 2015
Outros Autores: Queiroz, Artur Trancoso Lopo de, Tosta, Rafael, Carvalho, Augusto M., Barbosa, Carlos Henrique, Bellio, Maria, Oliveira, Camila Indiani de, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14091
Resumo: Universidade Estadual de Feira de Santana. Departamento de Tecnologia. Feira de Santana, BA, Brasil.
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spelling Duarte, ÂngeloQueiroz, Artur Trancoso Lopo deTosta, RafaelCarvalho, Augusto M.Barbosa, Carlos HenriqueBellio, MariaOliveira, Camila Indiani deBarral Netto, Manoel2016-05-04T16:53:37Z2016-05-04T16:53:37Z2015DUARTE, A. et al. Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation. PLoS One, v. 10, n. 6, p. 1-10,, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/14091engPublic Library of ScienceLeishmanioseLeishmania braziliensisEpitopos de celula TCelula T4Celula T8VacinasCorrection: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Feira de Santana. Departamento de Tecnologia. Feira de Santana, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Estadual de Feira de Santana. Departamento de Tecnologia. Feira de Santana, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil.Background: Leishmaniasis is caused by intracellular Leishmania parasites that induce a T-cell mediated response associated with recognition of CD4+ and CD8+ T cell Line 1Lineepitopes. Identification of CD8+ antigenic determinants is crucial for vaccine and therapy development. Herein, we developed an open-source software dedicated to search and compile data obtained from currently available on line prediction algorithms. Methodology/Principal Findings We developed a two-phase algorithm and implemented in an open source software called EPIBOT, that consolidates the results obtained with single prediction algorithms, generating a final output in which epitopes are ranked. EPIBOT was initially trained using a set of 831 known epitopes from 397 proteins from IEDB. We then screened 63 Leishmania braziliensis vaccine candidates with the EPIBOT trained tool to search for CD8+ T cell epitopes. A proof-of-concept experiment was conducted with the top eight CD8+ epitopes, elected by EPIBOT. To do this, the elected peptides were synthesized and validated for their in vivo cytotoxicity. Among the tested epitopes, three were able to induce lysis of pulsed-target cells. Conclusion: Our results show that EPIBOT can successfully search across existing prediction tools, generating a compiled list of candidate CD8+ epitopes. This software is fast and a simple search engine that can be customized to search over different MHC alleles or HLA haplotypes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14091/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDuarte A Prediction....pdfDuarte A Prediction....pdfapplication/pdf331586https://www.arca.fiocruz.br/bitstream/icict/14091/2/Duarte%20A%20Prediction....pdf3e8b32dd09b776e425e64b954f0cac90MD52TEXTDuarte A Prediction....pdf.txtDuarte A Prediction....pdf.txtExtracted texttext/plain36093https://www.arca.fiocruz.br/bitstream/icict/14091/3/Duarte%20A%20Prediction....pdf.txtf188d34cb334324f74490da5d573dc49MD53icict/140912023-03-15 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dc.title.pt_BR.fl_str_mv Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation
title Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation
spellingShingle Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation
Duarte, Ângelo
Leishmaniose
Leishmania braziliensis
Epitopos de celula T
Celula T4
Celula T8
Vacinas
title_short Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation
title_full Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation
title_fullStr Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation
title_full_unstemmed Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation
title_sort Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation
author Duarte, Ângelo
author_facet Duarte, Ângelo
Queiroz, Artur Trancoso Lopo de
Tosta, Rafael
Carvalho, Augusto M.
Barbosa, Carlos Henrique
Bellio, Maria
Oliveira, Camila Indiani de
Barral Netto, Manoel
author_role author
author2 Queiroz, Artur Trancoso Lopo de
Tosta, Rafael
Carvalho, Augusto M.
Barbosa, Carlos Henrique
Bellio, Maria
Oliveira, Camila Indiani de
Barral Netto, Manoel
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Duarte, Ângelo
Queiroz, Artur Trancoso Lopo de
Tosta, Rafael
Carvalho, Augusto M.
Barbosa, Carlos Henrique
Bellio, Maria
Oliveira, Camila Indiani de
Barral Netto, Manoel
dc.subject.other.pt_BR.fl_str_mv Leishmaniose
Leishmania braziliensis
Epitopos de celula T
Celula T4
Celula T8
Vacinas
topic Leishmaniose
Leishmania braziliensis
Epitopos de celula T
Celula T4
Celula T8
Vacinas
description Universidade Estadual de Feira de Santana. Departamento de Tecnologia. Feira de Santana, BA, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-05-04T16:53:37Z
dc.date.available.fl_str_mv 2016-05-04T16:53:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DUARTE, A. et al. Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation. PLoS One, v. 10, n. 6, p. 1-10,, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14091
dc.identifier.issn.none.fl_str_mv 1932-6203
identifier_str_mv DUARTE, A. et al. Correction: Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation. PLoS One, v. 10, n. 6, p. 1-10,, 2015.
1932-6203
url https://www.arca.fiocruz.br/handle/icict/14091
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/14091/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/14091/2/Duarte%20A%20Prediction....pdf
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