Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients

Detalhes bibliográficos
Autor(a) principal: Cantanhêde, Lilian Motta
Data de Publicação: 2015
Outros Autores: Júnior, Cipriano Ferreira da Silva, Ito, Marcos Massayuki, Felipin, Kátia Paula, Nicolete, Roberto, Salcedo, Juan Miguel Villalobos, Porrozzi, Renato, Cupolillo, Elisa, Ferreira, Ricardo de Godoi Mattos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12589
Resumo: Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.
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spelling Cantanhêde, Lilian MottaJúnior, Cipriano Ferreira da SilvaIto, Marcos MassayukiFelipin, Kátia PaulaNicolete, RobertoSalcedo, Juan Miguel VillalobosPorrozzi, RenatoCupolillo, ElisaFerreira, Ricardo de Godoi Mattos2016-01-21T21:36:45Z2016-01-21T21:36:45Z2015CANTANHÊDE, Lilian Motta et al. Further Evidence of an Association between the Presence of Leishmania RNAVirus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients. PLoS Negl. Trop. Dis., v. 9, n. 9, p. 4-11, Sept. 15, 2015.https://www.arca.fiocruz.br/handle/icict/1258910.1371/journal.pntd.0004079engPLOS Neglected Tropical DiseasesFurther Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Porto Velho, RO, Brasil.Governo do Estado de Rondônia. Secretaria de Saúde. Porto Velho, RO, Brasil.Governo do Estado de Rondônia. Secretaria de Saúde. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Tegumentary Leishmaniasis (TL) is endemic in Latin America, and Brazil contributes approximately 20 thousand cases per year. The pathogenesis of TL, however, is still not fully understood. Clinical manifestations vary from cutaneous leishmaniasis (CL) to more severe outcomes, such as disseminated leishmaniasis (DL), mucosal leishmaniasis (ML) and diffuse cutaneous leishmaniasis (DCL). Many factors have been associated with the severity of the disease and the development of lesions. Recent studies have reported that the presence of Leishmania RNA virus 1 infecting Leishmania (Leishmania RNA virus 1, LRV1) is an important factor associated with the severity of ML in experimental animal models. In the present study, 156 patients who attended Rondonia's Hospital of Tropical Medicine with both leishmaniasis clinical diagnoses (109 CL; 38 ML; 5 CL+ML; 3 DL and 1 DCL) and molecular diagnoses were investigated. The clinical diagnosis were confirmed by PCR by targeting hsp70 and kDNA DNA sequences and the species causing the infection were determined by HSP70 PCR-RFPL. The presence of LVR1 was tested by RT-PCR. Five Leishmania species were detected: 121 (77.6%) samples were positive for Leishmania (Viannia) braziliensis, 18 (11.5%) were positive for Leishmania (V.) guyanensis, 3 (1.8%) for Leishmania (V.) lainsoni, 2 (1.3%) for Leishmania (Leishmania) amazonensis and 2 (1.3%) for Leishmania (V.) shawi. Six (3.9%) samples were positive for Leishmania sp. but the species could not be determined, and 4 (2.6%) samples were suggestive of mixed infection by L. (V.) braziliensis and L. (V.) guyanensis. The virus was detected in L. braziliensis (N = 54), L. guyanensis (N = 5), L. amazonensis (N = 2), L. lainsoni (N = 1) and inconclusive samples (N = 6). Patients presenting with CL+ML, DL and DCL were excluded from further analysis. Association between the presence of the virus and the disease outcome were tested among the remaining 147 patients (CL = 109 and ML = 38). Of them, 71.1% (n = 27) mucosal lesions were positive for LRV1, and 28.9% (n = 11) were negative. In cutaneous lesions, 36.7%(n = 40) were positive and 63.3%(n = 69) were negative for LRV1. The ratio P(ML|LRV1+)/P(ML|LRV1-) was 2.93 (CI95%1.57. . .5.46; p<0.001), thus corroborating the hypothesis of the association between LRV1 and the occurrence of mucosal leishmaniasis, as previously described in animal models; it also indicates that LRV1 is not the only factor contributing to the disease outcome.LeishmaniaRNA Virus 1Mucosal ManifestationsTegumentaryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12589/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFurther Evidence of an Association.pdfFurther Evidence of an Association.pdfapplication/pdf863117https://www.arca.fiocruz.br/bitstream/icict/12589/2/Further%20Evidence%20of%20an%20Association.pdf7a312093f7956b74c7daaae042a635cdMD52TEXTFurther Evidence of an Association.pdf.txtFurther Evidence of an 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dc.title.pt_BR.fl_str_mv Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
title Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
spellingShingle Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
Cantanhêde, Lilian Motta
Leishmania
RNA Virus 1
Mucosal Manifestations
Tegumentary
title_short Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
title_full Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
title_fullStr Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
title_full_unstemmed Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
title_sort Further Evidence of an Association between the Presence of Leishmania RNA Virus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients
author Cantanhêde, Lilian Motta
author_facet Cantanhêde, Lilian Motta
Júnior, Cipriano Ferreira da Silva
Ito, Marcos Massayuki
Felipin, Kátia Paula
Nicolete, Roberto
Salcedo, Juan Miguel Villalobos
Porrozzi, Renato
Cupolillo, Elisa
Ferreira, Ricardo de Godoi Mattos
author_role author
author2 Júnior, Cipriano Ferreira da Silva
Ito, Marcos Massayuki
Felipin, Kátia Paula
Nicolete, Roberto
Salcedo, Juan Miguel Villalobos
Porrozzi, Renato
Cupolillo, Elisa
Ferreira, Ricardo de Godoi Mattos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cantanhêde, Lilian Motta
Júnior, Cipriano Ferreira da Silva
Ito, Marcos Massayuki
Felipin, Kátia Paula
Nicolete, Roberto
Salcedo, Juan Miguel Villalobos
Porrozzi, Renato
Cupolillo, Elisa
Ferreira, Ricardo de Godoi Mattos
dc.subject.en.eng.fl_str_mv Leishmania
RNA Virus 1
Mucosal Manifestations
Tegumentary
topic Leishmania
RNA Virus 1
Mucosal Manifestations
Tegumentary
description Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-21T21:36:45Z
dc.date.available.fl_str_mv 2016-01-21T21:36:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CANTANHÊDE, Lilian Motta et al. Further Evidence of an Association between the Presence of Leishmania RNAVirus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients. PLoS Negl. Trop. Dis., v. 9, n. 9, p. 4-11, Sept. 15, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12589
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0004079
identifier_str_mv CANTANHÊDE, Lilian Motta et al. Further Evidence of an Association between the Presence of Leishmania RNAVirus 1 and the Mucosal Manifestations in Tegumentary Leishmaniasis Patients. PLoS Negl. Trop. Dis., v. 9, n. 9, p. 4-11, Sept. 15, 2015.
10.1371/journal.pntd.0004079
url https://www.arca.fiocruz.br/handle/icict/12589
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv PLOS Neglected Tropical Diseases
publisher.none.fl_str_mv PLOS Neglected Tropical Diseases
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12589/1/license.txt
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