Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.actatropica.2013.11.026 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13716 |
Resumo: | 2017-11-30 |
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Gonçalves, Alessandra QueirogaAbellana, RosaSilva, Hélio Doyle Pereira daSantos, IvanildesSerra, Paula TaquitaJulião, Genimar RebouçasOrlandi, Patricia PuccinelliAscaso, Carlos2016-04-12T13:42:27Z2014GONÇALVES, Alessandra Queiroga et al. Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard. Acta Tropica, v. 131, p. 63-70, 2014.https://www.arca.fiocruz.br/handle/icict/1371610.1016/j.actatropica.2013.11.026engElsevierComparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standardinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2017-11-30Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, Amazonas, Brazil. / Universitat de Barcelona. Departament de Salut Pública. Facultat de Medicina. Barcelona, Spain.Universitat de Barcelona. Departament de Salut Pública. Facultat de Medicina. Barcelona, Spain.Universitat de Barcelona. Departament de Salut Pública. Facultat de Medicina. Barcelona, Spain.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, Amazonas, Brazil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, Amazonas, Brazil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, Amazonas, Brazil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, Amazonas, Brazil.Universitat de Barcelona. Departament de Salut Pública. Facultat de Medicina. Barcelona, Spain. / Institut d’Investigacions Biomèdiques August Pi i Sunyer. Barcelona, Spain.Performance evaluation of diagnostic tests is critical in the search for accurate diagnoses. A gold standard test is usually absent in parasitology, thus rendering satisfactory assessment of diagnostic accuracy difficult. Moreover, reliability (assessed by the study of repeatability) is a rarely studied characteristic of diagnostic tests. This study compared and evaluated the performance (repeatability, concordance and accuracy) of the spontaneous sedimentation technique (SST) and the Paratest for the diagnosis of Giardia lamblia, Entamoeba histolytica complex, Blastocystis spp., Ascaris lumbricoides, hookworm, Trichuristrichiura and Calodium hepaticum. Fecal samples of 143 individuals were separated into three replicates for each test. Concordance and homogeneity of the results between replicates of each test and between tests were evaluated. Proportions of positives, sensitivity and specificity were estimated using a Bayesian Latent Class Model. High repeatability of both tests was found for the detection of intestinal parasites, except for Blastocystis spp. and hookworm. Concordance between tests was generally high (concordance correlation coefficient, 0.72–0.88), except for Blastocystis spp., hookworm and T. trichiura. The Paratest detected more cases of Blastocystis spp. and fewer of hookworm than the SST. The tests were quite discordant in the detection of T. trichiura. A low sensitivity (39.4–49.2% for SST, 35.8–53.8% for Paratest) and a high specificity (93.2–97.2%) were found for both tests. The Paratest presented a slightly higher sensitivity for the diagnosis of Blastocystis spp. (53.8%), and SST did so for hookworm (49.2%). This is the first study on repeatability and accuracy (using a Bayesian approach) of two spontaneous sedimentation techniques. These results suggest underdiagnosis of little dense parasitic forms due to technical limitations in both tests. We conclude that the combined study of repeatability, concordance and accuracy is a key strategy for better evaluation of the performance of tests and is also useful for the identification of technical limitations.Intestinal parasitesSpontaneous sedimentation techniquesDiagnosisRepeatabilityAccuracyBayesian analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALComparison of the performance.pdfComparison of the performance.pdfapplication/pdf898884https://www.arca.fiocruz.br/bitstream/icict/13716/2/Comparison%20of%20the%20performance.pdf94d6333e1581ab498e8500e93c971d10MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13716/3/license.txt5a560609d32a3863062d77ff32785d58MD53TEXTComparison of the performance.pdf.txtComparison of the performance.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard |
| title |
Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard |
| spellingShingle |
Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard Gonçalves, Alessandra Queiroga Intestinal parasites Spontaneous sedimentation techniques Diagnosis Repeatability Accuracy Bayesian analysis |
| title_short |
Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard |
| title_full |
Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard |
| title_fullStr |
Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard |
| title_full_unstemmed |
Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard |
| title_sort |
Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard |
| author |
Gonçalves, Alessandra Queiroga |
| author_facet |
Gonçalves, Alessandra Queiroga Abellana, Rosa Silva, Hélio Doyle Pereira da Santos, Ivanildes Serra, Paula Taquita Julião, Genimar Rebouças Orlandi, Patricia Puccinelli Ascaso, Carlos |
| author_role |
author |
| author2 |
Abellana, Rosa Silva, Hélio Doyle Pereira da Santos, Ivanildes Serra, Paula Taquita Julião, Genimar Rebouças Orlandi, Patricia Puccinelli Ascaso, Carlos |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gonçalves, Alessandra Queiroga Abellana, Rosa Silva, Hélio Doyle Pereira da Santos, Ivanildes Serra, Paula Taquita Julião, Genimar Rebouças Orlandi, Patricia Puccinelli Ascaso, Carlos |
| dc.subject.en.pt_BR.fl_str_mv |
Intestinal parasites Spontaneous sedimentation techniques Diagnosis Repeatability Accuracy Bayesian analysis |
| topic |
Intestinal parasites Spontaneous sedimentation techniques Diagnosis Repeatability Accuracy Bayesian analysis |
| description |
2017-11-30 |
| publishDate |
2014 |
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2014 |
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2016-04-12T13:42:27Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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GONÇALVES, Alessandra Queiroga et al. Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard. Acta Tropica, v. 131, p. 63-70, 2014. |
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https://www.arca.fiocruz.br/handle/icict/13716 |
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10.1016/j.actatropica.2013.11.026 |
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GONÇALVES, Alessandra Queiroga et al. Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard. Acta Tropica, v. 131, p. 63-70, 2014. 10.1016/j.actatropica.2013.11.026 |
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Elsevier |
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Elsevier |
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