Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/43107 |
Resumo: | Platynosomiasis is a parasitic infection reported in non-human primates, including marmosets, and is frequently difficult to diagnose. In this study, the Kato-Katz method and the spontaneous sedimentation method were evaluated for their usefulness in identifying Platynosomum eggs in fecal samples from Callithrix penicillata that naturally harbor Platynosomum illiciens. Spontaneous sedimentation allowed the diagnosis of 41.7% (5/12) and 66.7% (8/12) of infected marmosets from one and three slides, respectively, prepared from the same fecal sample. The examination of a single Kato-Katz thick smear detected 83.3% (10/12) of infection cases. The analysis of feces on three different days increased the rate of diagnosis, since 75% (9/12) and 100% (12/12) of the primates with platynosomiasis were identified using serial spontaneous sedimentation (3 slides/day) and the Kato-Katz method, respectively. The mean number of Platynosomum eggs per gram of feces determined via the Kato-Katz method was 71.7 (8-240). The spontaneous sedimentation method when performed in series is acceptable for the diagnosis of platynosomiasis. However, the Kato-Katz method, which was here used for the first time to detect this infection, has a higher diagnostic sensitivity and the advantage that a quantitative analysis of the eggs released in the host feces is possible. |
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Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primatesAvaliação dos métodos de sedimentação espontânea e Kato-Katz para o diagnóstico da platinossomose em primatas neotropicaisFecal parasitological diagnosisPlatynosomiasisPlatynosomumMarmosetsSpontaneous sedimentationKato-Katz thick smearPlatynosomiasis is a parasitic infection reported in non-human primates, including marmosets, and is frequently difficult to diagnose. In this study, the Kato-Katz method and the spontaneous sedimentation method were evaluated for their usefulness in identifying Platynosomum eggs in fecal samples from Callithrix penicillata that naturally harbor Platynosomum illiciens. Spontaneous sedimentation allowed the diagnosis of 41.7% (5/12) and 66.7% (8/12) of infected marmosets from one and three slides, respectively, prepared from the same fecal sample. The examination of a single Kato-Katz thick smear detected 83.3% (10/12) of infection cases. The analysis of feces on three different days increased the rate of diagnosis, since 75% (9/12) and 100% (12/12) of the primates with platynosomiasis were identified using serial spontaneous sedimentation (3 slides/day) and the Kato-Katz method, respectively. The mean number of Platynosomum eggs per gram of feces determined via the Kato-Katz method was 71.7 (8-240). The spontaneous sedimentation method when performed in series is acceptable for the diagnosis of platynosomiasis. However, the Kato-Katz method, which was here used for the first time to detect this infection, has a higher diagnostic sensitivity and the advantage that a quantitative analysis of the eggs released in the host feces is possible.A platinossomose é uma infecção parasitária relatada em primatas não-humanos, inclusive saguis, cujo diagnóstico é frequentemente difícil. Neste estudo, os métodos de sedimentação espontânea e Kato-Katz foram avaliados quanto à sua utilidade na identificação de ovos de Platynosomum em amostras fecais de Callithrix penicillata naturalmente albergando Platynosomum illiciens. A sedimentação espontânea permitiu o diagnóstico de 41,7% (5/12) e 66,7% (8/12) dos saguis infectados a partir da análise de uma e três lâminas, respectivamente, preparadas de uma mesma amostra fecal. O exame de uma única lâmina de Kato-Katz detectou 83,3% (10/12) dos casos de infecção. A análise de fezes em três dias diferentes aumentou as taxas de diagnóstico, uma vez que 75% (9/12) e 100% (12/12) dos primatas que apresentaram a platinossomose foram identificados, usando-se a sedimentação espontânea (três lâminas/dia) e o Kato-Katz em série, respectivamente. O número médio de ovos de Platynosomum por g de fezes, determinado através do método de Kato-Katz, foi de 71,7 (8-240). O método de sedimentação espontânea, quando realizado em série, é aceitável para o diagnóstico da platinossomose. Entretanto, o método de Kato-Katz, o qual foi pela primeira vez usado para se detectar essa infecção, mostrou uma maior sensibilidade diagnóstica, com a vantagem de que é possível uma análise quantitativa dos ovos liberados nas fezes do hospedeiro.Colégio Brasileiro de Parasitologia Veterinária (CBPV)2020-09-17T01:15:37Z2020-09-17T01:15:37Z2015-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMATI, V. L. T.; PINTO, H.A.; MELO, A. L. de. Evaluation of the accuracy of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 24, n. 1, p. 108-113, Jan./Mar. 2015. DOI: 10.1590/S1984-29612015014.http://repositorio.ufla.br/jspui/handle/1/43107Revista Brasileira de Parasitologia Veterinária (RBPV)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessTenório, Vitor LuísPinto, Hudson AlvesMelo, Alan Lane deeng2020-09-17T01:15:38Zoai:localhost:1/43107Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-09-17T01:15:38Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates Avaliação dos métodos de sedimentação espontânea e Kato-Katz para o diagnóstico da platinossomose em primatas neotropicais |
title |
Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates |
spellingShingle |
Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates Tenório, Vitor Luís Fecal parasitological diagnosis Platynosomiasis Platynosomum Marmosets Spontaneous sedimentation Kato-Katz thick smear |
title_short |
Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates |
title_full |
Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates |
title_fullStr |
Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates |
title_full_unstemmed |
Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates |
title_sort |
Evaluation of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates |
author |
Tenório, Vitor Luís |
author_facet |
Tenório, Vitor Luís Pinto, Hudson Alves Melo, Alan Lane de |
author_role |
author |
author2 |
Pinto, Hudson Alves Melo, Alan Lane de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Tenório, Vitor Luís Pinto, Hudson Alves Melo, Alan Lane de |
dc.subject.por.fl_str_mv |
Fecal parasitological diagnosis Platynosomiasis Platynosomum Marmosets Spontaneous sedimentation Kato-Katz thick smear |
topic |
Fecal parasitological diagnosis Platynosomiasis Platynosomum Marmosets Spontaneous sedimentation Kato-Katz thick smear |
description |
Platynosomiasis is a parasitic infection reported in non-human primates, including marmosets, and is frequently difficult to diagnose. In this study, the Kato-Katz method and the spontaneous sedimentation method were evaluated for their usefulness in identifying Platynosomum eggs in fecal samples from Callithrix penicillata that naturally harbor Platynosomum illiciens. Spontaneous sedimentation allowed the diagnosis of 41.7% (5/12) and 66.7% (8/12) of infected marmosets from one and three slides, respectively, prepared from the same fecal sample. The examination of a single Kato-Katz thick smear detected 83.3% (10/12) of infection cases. The analysis of feces on three different days increased the rate of diagnosis, since 75% (9/12) and 100% (12/12) of the primates with platynosomiasis were identified using serial spontaneous sedimentation (3 slides/day) and the Kato-Katz method, respectively. The mean number of Platynosomum eggs per gram of feces determined via the Kato-Katz method was 71.7 (8-240). The spontaneous sedimentation method when performed in series is acceptable for the diagnosis of platynosomiasis. However, the Kato-Katz method, which was here used for the first time to detect this infection, has a higher diagnostic sensitivity and the advantage that a quantitative analysis of the eggs released in the host feces is possible. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03 2020-09-17T01:15:37Z 2020-09-17T01:15:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MATI, V. L. T.; PINTO, H.A.; MELO, A. L. de. Evaluation of the accuracy of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 24, n. 1, p. 108-113, Jan./Mar. 2015. DOI: 10.1590/S1984-29612015014. http://repositorio.ufla.br/jspui/handle/1/43107 |
identifier_str_mv |
MATI, V. L. T.; PINTO, H.A.; MELO, A. L. de. Evaluation of the accuracy of Kato-Katz and spontaneous sedimentation methods for the diagnosis of platynosomiasis in neotropical primates. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 24, n. 1, p. 108-113, Jan./Mar. 2015. DOI: 10.1590/S1984-29612015014. |
url |
http://repositorio.ufla.br/jspui/handle/1/43107 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (RBPV) reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1784550097564991488 |