Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7427 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil |
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Damasceno, Leonardo MirandaHuang, C. J.Batt, C. A.2014-03-19T18:13:59Z2014-03-19T18:13:59Z2012DAMASCENO, Leonardo Miranda; HUANG, CJ; BATT, CA. Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology. 2012, vol.93, n.1, pp. 31-39.0175-7598https://www.arca.fiocruz.br/handle/icict/742710.1007/s00253-011-3654-zengSpringer LinkProtein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilCornell University. Department of Microbiolog. Ithaca, NY, USACornell University. Department of Microbiolog. Ithaca, NY, USAYeast expression systems have been successfully used for over 20 years for the production of recombinant proteins. With the growing interest in recombinant protein expression for various uses, yeast expression systems, such as the popular Pichia pastoris, are becoming increasingly important. Although P. pastoris has been successfully used in the production of many secreted and intracellular recombinant proteins, there is still room for improvement of this expression system. In particular, secretion of recombinant proteins is still one of the main reasons for using P. pastoris. Therefore, endoplasmic reticulum protein folding, correct glycosylation, vesicular transport to the plasma membrane, gene dosage, secretion signal sequences, and secretome studies are important considerations for improved recombinant protein production.Pichia pastorisRecombinant protein secretionUnfolded protein responseProtein foldinginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALProtein secretion in Pichia pastoris and advances in protein production.pdfProtein secretion in Pichia pastoris and advances in protein production.pdfapplication/pdf264431https://www.arca.fiocruz.br/bitstream/icict/7427/1/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf59b48b61eb1be20befaa979cbc967496MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7427/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTProtein secretion in Pichia pastoris and advances in protein production.pdf.txtProtein secretion in Pichia pastoris and advances in protein production.pdf.txtExtracted texttext/plain46873https://www.arca.fiocruz.br/bitstream/icict/7427/5/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf.txta0c60d4be505efb1573acc980cfeaa61MD55THUMBNAILProtein secretion in Pichia pastoris and advances in protein production.pdf.jpgProtein secretion in Pichia pastoris and advances in protein production.pdf.jpgGenerated Thumbnailimage/jpeg1945https://www.arca.fiocruz.br/bitstream/icict/7427/4/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf.jpg0ca0ad9ae8c1c93d68ae941fff9d9c44MD54icict/74272023-07-10 11:15:53.369oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-10T14:15:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology |
title |
Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology |
spellingShingle |
Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology Damasceno, Leonardo Miranda Pichia pastoris Recombinant protein secretion Unfolded protein response Protein folding |
title_short |
Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology |
title_full |
Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology |
title_fullStr |
Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology |
title_full_unstemmed |
Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology |
title_sort |
Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology |
author |
Damasceno, Leonardo Miranda |
author_facet |
Damasceno, Leonardo Miranda Huang, C. J. Batt, C. A. |
author_role |
author |
author2 |
Huang, C. J. Batt, C. A. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Damasceno, Leonardo Miranda Huang, C. J. Batt, C. A. |
dc.subject.en.pt_BR.fl_str_mv |
Pichia pastoris Recombinant protein secretion Unfolded protein response Protein folding |
topic |
Pichia pastoris Recombinant protein secretion Unfolded protein response Protein folding |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2014-03-19T18:13:59Z |
dc.date.available.fl_str_mv |
2014-03-19T18:13:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DAMASCENO, Leonardo Miranda; HUANG, CJ; BATT, CA. Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology. 2012, vol.93, n.1, pp. 31-39. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7427 |
dc.identifier.issn.none.fl_str_mv |
0175-7598 |
dc.identifier.doi.none.fl_str_mv |
10.1007/s00253-011-3654-z |
identifier_str_mv |
DAMASCENO, Leonardo Miranda; HUANG, CJ; BATT, CA. Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology. 2012, vol.93, n.1, pp. 31-39. 0175-7598 10.1007/s00253-011-3654-z |
url |
https://www.arca.fiocruz.br/handle/icict/7427 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Springer Link |
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Springer Link |
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