Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology

Detalhes bibliográficos
Autor(a) principal: Damasceno, Leonardo Miranda
Data de Publicação: 2012
Outros Autores: Huang, C. J., Batt, C. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7427
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
id CRUZ_145990dcabf90355f0b3e2efa12eeb42
oai_identifier_str oai:www.arca.fiocruz.br:icict/7427
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Damasceno, Leonardo MirandaHuang, C. J.Batt, C. A.2014-03-19T18:13:59Z2014-03-19T18:13:59Z2012DAMASCENO, Leonardo Miranda; HUANG, CJ; BATT, CA. Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology. 2012, vol.93, n.1, pp. 31-39.0175-7598https://www.arca.fiocruz.br/handle/icict/742710.1007/s00253-011-3654-zengSpringer LinkProtein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilCornell University. Department of Microbiolog. Ithaca, NY, USACornell University. Department of Microbiolog. Ithaca, NY, USAYeast expression systems have been successfully used for over 20 years for the production of recombinant proteins. With the growing interest in recombinant protein expression for various uses, yeast expression systems, such as the popular Pichia pastoris, are becoming increasingly important. Although P. pastoris has been successfully used in the production of many secreted and intracellular recombinant proteins, there is still room for improvement of this expression system. In particular, secretion of recombinant proteins is still one of the main reasons for using P. pastoris. Therefore, endoplasmic reticulum protein folding, correct glycosylation, vesicular transport to the plasma membrane, gene dosage, secretion signal sequences, and secretome studies are important considerations for improved recombinant protein production.Pichia pastorisRecombinant protein secretionUnfolded protein responseProtein foldinginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALProtein secretion in Pichia pastoris and advances in protein production.pdfProtein secretion in Pichia pastoris and advances in protein production.pdfapplication/pdf264431https://www.arca.fiocruz.br/bitstream/icict/7427/1/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf59b48b61eb1be20befaa979cbc967496MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7427/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTProtein secretion in Pichia pastoris and advances in protein production.pdf.txtProtein secretion in Pichia pastoris and advances in protein production.pdf.txtExtracted texttext/plain46873https://www.arca.fiocruz.br/bitstream/icict/7427/5/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf.txta0c60d4be505efb1573acc980cfeaa61MD55THUMBNAILProtein secretion in Pichia pastoris and advances in protein production.pdf.jpgProtein secretion in Pichia pastoris and advances in protein production.pdf.jpgGenerated Thumbnailimage/jpeg1945https://www.arca.fiocruz.br/bitstream/icict/7427/4/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf.jpg0ca0ad9ae8c1c93d68ae941fff9d9c44MD54icict/74272023-07-10 11:15:53.369oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-10T14:15:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
title Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
spellingShingle Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
Damasceno, Leonardo Miranda
Pichia pastoris
Recombinant protein secretion
Unfolded protein response
Protein folding
title_short Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
title_full Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
title_fullStr Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
title_full_unstemmed Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
title_sort Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology
author Damasceno, Leonardo Miranda
author_facet Damasceno, Leonardo Miranda
Huang, C. J.
Batt, C. A.
author_role author
author2 Huang, C. J.
Batt, C. A.
author2_role author
author
dc.contributor.author.fl_str_mv Damasceno, Leonardo Miranda
Huang, C. J.
Batt, C. A.
dc.subject.en.pt_BR.fl_str_mv Pichia pastoris
Recombinant protein secretion
Unfolded protein response
Protein folding
topic Pichia pastoris
Recombinant protein secretion
Unfolded protein response
Protein folding
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-03-19T18:13:59Z
dc.date.available.fl_str_mv 2014-03-19T18:13:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DAMASCENO, Leonardo Miranda; HUANG, CJ; BATT, CA. Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology. 2012, vol.93, n.1, pp. 31-39.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7427
dc.identifier.issn.none.fl_str_mv 0175-7598
dc.identifier.doi.none.fl_str_mv 10.1007/s00253-011-3654-z
identifier_str_mv DAMASCENO, Leonardo Miranda; HUANG, CJ; BATT, CA. Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology Biotechnology. 2012, vol.93, n.1, pp. 31-39.
0175-7598
10.1007/s00253-011-3654-z
url https://www.arca.fiocruz.br/handle/icict/7427
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Link
publisher.none.fl_str_mv Springer Link
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7427/1/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf
https://www.arca.fiocruz.br/bitstream/icict/7427/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7427/5/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/7427/4/Protein%20secretion%20in%20Pichia%20pastoris%20and%20advances%20in%20protein%20production.pdf.jpg
bitstream.checksum.fl_str_mv 59b48b61eb1be20befaa979cbc967496
7d48279ffeed55da8dfe2f8e81f3b81f
a0c60d4be505efb1573acc980cfeaa61
0ca0ad9ae8c1c93d68ae941fff9d9c44
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324682652385280

Registros relacionados