A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Silva, Maria Almerice Lopes da
Data de Publicação: 2014
Outros Autores: Medeiros, Zulma, Soares, Cynthia Regina Pedrosa, Silva, Elis Dionísio da, Miranda-Filho, Demócrito Barros, Melo, Fábio Lopes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23320
Resumo: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Doenças Transmissíveis. Recife, PE, Brasil.
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spelling Silva, Maria Almerice Lopes daMedeiros, ZulmaSoares, Cynthia Regina PedrosaSilva, Elis Dionísio daMiranda-Filho, Demócrito BarrosMelo, Fábio Lopes de2017-11-23T13:38:09Z2017-11-23T13:38:09Z2014SILVA, M. A. L. da et al. A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 2, p. 193–197, abr. 2014.1678-9849https://www.arca.fiocruz.br/handle/icict/2332010.1590/0037-8682-0233-2013engurinaExtraçãoDNALeishmaniose visceralUrineExtractionDNAVisceral leishmaniasisReação em Cadeia da PolimeraseHumanosDNA de ProtozoáriourinaLeishmania infantumgenéticaLeishmaniose VisceralparasitologiaManejo de EspécimesmétodosLeishmania infantumisolamento & purificaçãoLeishmaniose VisceralurinaA comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Doenças Transmissíveis. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Doenças Transmissíveis. Recife, PE, Brasil / Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Doenças Transmissíveis. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Doenças Transmissíveis. Recife, PE, Brasil.Universidade de Pernambuco. Faculdade de Ciências Médicas. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Doenças Transmissíveis. Recife, PE, Brasil.Polymerase chain reaction (PCR) may offer an alternative diagnostic option when clinical signs and symptoms suggest visceral leishmaniasis (VL) but microscopic scanning and serological tests provide negative results. PCR using urine is sensitive enough to diagnose human visceral leishmaniasis (VL). However, DNA quality is a crucial factor for successful amplification.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23320/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. A comparison of four DNA - silva.pdfart. A comparison of four DNA - silva.pdfapplication/pdf1028807https://www.arca.fiocruz.br/bitstream/icict/23320/2/art.%20A%20comparison%20of%20four%20DNA%20-%20silva.pdfec8ae7831502fd08048c342b7c243f55MD52TEXTart. A comparison of four DNA - silva.pdf.txtart. 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dc.title.pt_BR.fl_str_mv A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
title A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
spellingShingle A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
Silva, Maria Almerice Lopes da
urina
Extração
DNA
Leishmaniose visceral
Urine
Extraction
DNA
Visceral leishmaniasis
Reação em Cadeia da Polimerase
Humanos
DNA de Protozoário
urina
Leishmania infantum
genética
Leishmaniose Visceral
parasitologia
Manejo de Espécimes
métodos
Leishmania infantum
isolamento & purificação
Leishmaniose Visceral
urina
title_short A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
title_full A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
title_fullStr A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
title_full_unstemmed A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
title_sort A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
author Silva, Maria Almerice Lopes da
author_facet Silva, Maria Almerice Lopes da
Medeiros, Zulma
Soares, Cynthia Regina Pedrosa
Silva, Elis Dionísio da
Miranda-Filho, Demócrito Barros
Melo, Fábio Lopes de
author_role author
author2 Medeiros, Zulma
Soares, Cynthia Regina Pedrosa
Silva, Elis Dionísio da
Miranda-Filho, Demócrito Barros
Melo, Fábio Lopes de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Maria Almerice Lopes da
Medeiros, Zulma
Soares, Cynthia Regina Pedrosa
Silva, Elis Dionísio da
Miranda-Filho, Demócrito Barros
Melo, Fábio Lopes de
dc.subject.other.pt_BR.fl_str_mv urina
Extração
DNA
Leishmaniose visceral
topic urina
Extração
DNA
Leishmaniose visceral
Urine
Extraction
DNA
Visceral leishmaniasis
Reação em Cadeia da Polimerase
Humanos
DNA de Protozoário
urina
Leishmania infantum
genética
Leishmaniose Visceral
parasitologia
Manejo de Espécimes
métodos
Leishmania infantum
isolamento & purificação
Leishmaniose Visceral
urina
dc.subject.en.pt_BR.fl_str_mv Urine
Extraction
DNA
Visceral leishmaniasis
dc.subject.decs.pt_BR.fl_str_mv Reação em Cadeia da Polimerase
Humanos
DNA de Protozoário
urina
Leishmania infantum
genética
Leishmaniose Visceral
parasitologia
Manejo de Espécimes
métodos
Leishmania infantum
isolamento & purificação
Leishmaniose Visceral
urina
description Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Doenças Transmissíveis. Recife, PE, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2017-11-23T13:38:09Z
dc.date.available.fl_str_mv 2017-11-23T13:38:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, M. A. L. da et al. A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 2, p. 193–197, abr. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23320
dc.identifier.issn.pt_BR.fl_str_mv 1678-9849
dc.identifier.doi.none.fl_str_mv 10.1590/0037-8682-0233-2013
identifier_str_mv SILVA, M. A. L. da et al. A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 2, p. 193–197, abr. 2014.
1678-9849
10.1590/0037-8682-0233-2013
url https://www.arca.fiocruz.br/handle/icict/23320
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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institution FIOCRUZ
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