Comparison of five methods for the determination of lethal dose in acute toxicity studies
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1989 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11351 |
Resumo: | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil. |
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Paumgartten, Francisco José RomaPresgrave, Octavio Augusto FrançaMenezes, Marco Antônio CarneiroFingola, Fernando FariaFreitas, João Carlos Borges Rolim deCarvalho, Rosangela Ribeiro deCunha, Fernando de Queiroz2015-07-14T00:47:04Z2015-07-14T00:47:04Z1989PAUMGARTTEN, F. J. R. et al. Comparison of five methods for the determination of lethal dose in acute toxicity studies. Braz. J. Med. Biol. Res. São Paulo, v. 22, n. 8, p. 987-991, 1989.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/11351engAssociação Brasileira de Divulgação CientíficaAlternativas aos Testes com AnimaisHexaclorofenoDose Letal MedianaLidocaínaCamundongosFenobarbitalFisostigminaAnimal Testing AlternativesHexachloropheneLethal Dose 50LidocaineMicePhenobarbitalPhysostigmineAlternativas aos Testes com AnimaisHexaclorofenoDose Letal MedianaLidocaínaCamundongosFenobarbitalFisostigminaComparison of five methods for the determination of lethal dose in acute toxicity studiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil.The aim of the present study was to compare the reliability of LD50 determination using the traditional Litchfield and Wilcoxon method with that obtained by four alternative tests requiring smaller numbers of animals, for the purpose of classifying chemicals according to their acute toxicity. Acute lethal dose determinations were carried out in mice for oral and intraperitoneal administration of hexachlorophene, lidocaine, methanol, phenobarbital and physostigmine. The Molinengo method proved not to be as reliable as suggested by its author. Determination of LD50 using the Thompson and Weil method or, alternatively, the maximal non-lethal dose and the approximate lethal dose permitted the classification of the chemicals in essentially the same order. The approximate lethal dose method, in particular, seems to be a very suitable alternative method to the classical LD50 test since it requires only about 6 animals, provides enough information to order chemicals according to their toxicities, and provides useful information for planning subsequent repeated-dose studies.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11351/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALBraz J Med Biol Res_22_987-991.pdfBraz J Med Biol Res_22_987-991.pdfapplication/pdf1417412https://www.arca.fiocruz.br/bitstream/icict/11351/2/Braz%20J%20Med%20Biol%20Res_22_987-991.pdfa3c8a5edf49fe23be9797e213fa61985MD52TEXTBraz J Med Biol Res_22_987-991.pdf.txtBraz J Med Biol Res_22_987-991.pdf.txtExtracted texttext/plain5https://www.arca.fiocruz.br/bitstream/icict/11351/3/Braz%20J%20Med%20Biol%20Res_22_987-991.pdf.txtcc9067c2ee470dc248b14b194209a34eMD53icict/113512018-04-04 08:06:20.753oai:www.arca.fiocruz.br:icict/11351Q0VTU8ODTyBOw4NPLUVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcyBhdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKQ0VERSBlIFRSQU5TRkVSRSwgdG90YWwgZSBncmF0dWl0YW1lbnRlLCDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVosIGVtIGNhcsOhdGVyIHBlcm1hbmVudGUsIGlycmV2b2fDoXZlbCBlIE7Dg08gRVhDTFVTSVZPLCAKdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08gQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcyAKZGUgdm96IGUgaW1hZ2VtIHZpbmN1bGFkb3Mgw6AgT0JSQSwgZHVyYW50ZSB0b2RvIG8gcHJhem8gZGUgZHVyYcOnw6NvIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgZW0gcXVhbHF1ZXIgaWRpb21hIGUgZW0gdG9kb3Mgb3MgcGHDrXNlczsKICAgICAgICAKQUNFSVRBIHF1ZSBhIGNlc3PDo28gdG90YWwgbsOjbyBleGNsdXNpdmEsIHBlcm1hbmVudGUgZSBpcnJldm9nw6F2ZWwgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhdHJpbW9uaWFpcyBuw6NvIGNvbWVyY2lhaXMgZGUgdXRpbGl6YcOnw6NvIApkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwgb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciAKbWVpbyBvdSB2ZcOtY3VsbywgaW5jbHVzaXZlIGVtIFJlcG9zaXTDs3Jpb3MgRGlnaXRhaXMsIGJlbSBjb21vIG9zIGRpcmVpdG9zIGRlIHJlcHJvZHXDp8OjbywgZXhpYmnDp8OjbywgZXhlY3XDp8OjbywgZGVjbGFtYcOnw6NvLCBleHBvc2nDp8OjbywgCmFycXVpdmFtZW50bywgaW5jbHVzw6NvIGVtIGJhbmNvIGRlIGRhZG9zLCBwcmVzZXJ2YcOnw6NvLCBkaWZ1c8OjbywgZGlzdHJpYnVpw6fDo28sIGRpdnVsZ2HDp8OjbywgZW1wcsOpc3RpbW8sIHRyYWR1w6fDo28sIGluY2x1c8OjbyBlbSBub3ZhcyAKb2JyYXMgb3UgY29sZXTDom5lYXMsIHJldXRpbGl6YcOnw6NvLCBlZGnDp8OjbywgcHJvZHXDp8OjbyBkZSBtYXRlcmlhbCBkaWTDoXRpY28gZSBjdXJzb3Mgb3UgcXVhbHF1ZXIgZm9ybWEgZGUgdXRpbGl6YcOnw6NvIG7Do28gY29tZXJjaWFsOwogICAgICAgIApSRUNPTkhFQ0UgcXVlIGEgY2Vzc8OjbyBhcXVpIGVzcGVjaWZpY2FkYSBjb25jZWRlIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiBvIGRpcmVpdG8gZGUgYXV0b3JpemFyIHF1YWxxdWVyIHBlc3NvYSDigJMgZsOtc2ljYSAKb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdSBlc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlciAKZmluYWxpZGFkZXMgbsOjbyBjb21lcmNpYWlzOwogICAgICAgIApERUNMQVJBIHF1ZSBhIG9icmEgw6kgY3JpYcOnw6NvIG9yaWdpbmFsIGUgcXVlIMOpIG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgYXF1aSBjZWRpZG9zIGUgYXV0b3JpemFkb3MsIHJlc3BvbnNhYmlsaXphbmRvLXNlIGludGVncmFsbWVudGUgCnBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwgaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIAppbmRlbml6YXIgdGVyY2Vpcm9zIHBvciBkYW5vcywgYmVtIGNvbW8gaW5kZW5pemFyIGUgcmVzc2FyY2lyIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiBkZSBldmVudHVhaXMgZGVzcGVzYXMgcXVlIHZpZXJlbSBhIApzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91IGRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIAplIHZpb2xhw6fDtWVzIGRlIGRpcmVpdG9zOwogICAgICAgIApBRklSTUEgcXVlIGNvbmhlY2UgYSBQb2zDrXRpY2EgSW5zdGl0dWNpb25hbCBkZSBBY2Vzc28gQWJlcnRvIGRhIEluc3RpdHVpw6fDo28gZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyAKaW5zdGl0dWNpb25hbCBBUkNBLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-04T11:06:20Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en.fl_str_mv |
Comparison of five methods for the determination of lethal dose in acute toxicity studies |
| title |
Comparison of five methods for the determination of lethal dose in acute toxicity studies |
| spellingShingle |
Comparison of five methods for the determination of lethal dose in acute toxicity studies Paumgartten, Francisco José Roma Alternativas aos Testes com Animais Hexaclorofeno Dose Letal Mediana Lidocaína Camundongos Fenobarbital Fisostigmina Animal Testing Alternatives Hexachlorophene Lethal Dose 50 Lidocaine Mice Phenobarbital Physostigmine Alternativas aos Testes com Animais Hexaclorofeno Dose Letal Mediana Lidocaína Camundongos Fenobarbital Fisostigmina |
| title_short |
Comparison of five methods for the determination of lethal dose in acute toxicity studies |
| title_full |
Comparison of five methods for the determination of lethal dose in acute toxicity studies |
| title_fullStr |
Comparison of five methods for the determination of lethal dose in acute toxicity studies |
| title_full_unstemmed |
Comparison of five methods for the determination of lethal dose in acute toxicity studies |
| title_sort |
Comparison of five methods for the determination of lethal dose in acute toxicity studies |
| author |
Paumgartten, Francisco José Roma |
| author_facet |
Paumgartten, Francisco José Roma Presgrave, Octavio Augusto França Menezes, Marco Antônio Carneiro Fingola, Fernando Faria Freitas, João Carlos Borges Rolim de Carvalho, Rosangela Ribeiro de Cunha, Fernando de Queiroz |
| author_role |
author |
| author2 |
Presgrave, Octavio Augusto França Menezes, Marco Antônio Carneiro Fingola, Fernando Faria Freitas, João Carlos Borges Rolim de Carvalho, Rosangela Ribeiro de Cunha, Fernando de Queiroz |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Paumgartten, Francisco José Roma Presgrave, Octavio Augusto França Menezes, Marco Antônio Carneiro Fingola, Fernando Faria Freitas, João Carlos Borges Rolim de Carvalho, Rosangela Ribeiro de Cunha, Fernando de Queiroz |
| dc.subject.other.pt_BR.fl_str_mv |
Alternativas aos Testes com Animais Hexaclorofeno Dose Letal Mediana Lidocaína Camundongos Fenobarbital Fisostigmina |
| topic |
Alternativas aos Testes com Animais Hexaclorofeno Dose Letal Mediana Lidocaína Camundongos Fenobarbital Fisostigmina Animal Testing Alternatives Hexachlorophene Lethal Dose 50 Lidocaine Mice Phenobarbital Physostigmine Alternativas aos Testes com Animais Hexaclorofeno Dose Letal Mediana Lidocaína Camundongos Fenobarbital Fisostigmina |
| dc.subject.en.en.fl_str_mv |
Animal Testing Alternatives Hexachlorophene Lethal Dose 50 Lidocaine Mice Phenobarbital Physostigmine |
| dc.subject.decs.pt_BR.fl_str_mv |
Alternativas aos Testes com Animais Hexaclorofeno Dose Letal Mediana Lidocaína Camundongos Fenobarbital Fisostigmina |
| description |
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil. |
| publishDate |
1989 |
| dc.date.issued.fl_str_mv |
1989 |
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2015-07-14T00:47:04Z |
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2015-07-14T00:47:04Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
PAUMGARTTEN, F. J. R. et al. Comparison of five methods for the determination of lethal dose in acute toxicity studies. Braz. J. Med. Biol. Res. São Paulo, v. 22, n. 8, p. 987-991, 1989. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/11351 |
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0100-879X |
| identifier_str_mv |
PAUMGARTTEN, F. J. R. et al. Comparison of five methods for the determination of lethal dose in acute toxicity studies. Braz. J. Med. Biol. Res. São Paulo, v. 22, n. 8, p. 987-991, 1989. 0100-879X |
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