Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31797 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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Robert, Anny WaloskiAngulski, Addeli Bez BattiSpangenberg, LuciaShigunov, PatríciaPereira, Isabela TiemyBettes, Paulo Sergio LoiaconoNaya, HugoDominguez, Alejandro CorreaDallagiovanna, BrunoStimamiglio, Marco Augusto2019-02-20T19:47:52Z2019-02-20T19:47:52Z2018ROBERT, Anny Waloski et al. Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis. Scientific Reports, v. 8, n. 1, p. 1-11, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3179710.1038/s41598-018-22991-6porNature ResearchCélulas-tronco mesenquimaisMesenchymal Stromal CellsMesenchymal stem cellsOsteogenesisGene Expression RegulationCélulas del Estroma MesenquimatosoOsteogénesisRegulación de la Expresión GénicaCélulas Mesenquimais EstromaisOsteogêneseRegulação da Expressão GênicaGene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Pasteur. Unidade de Bioinformática. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Cirurgia Plástica Dr. Paulo Bettes. Curitiba, PR, Brasil.Instituto Pasteur. Unidade de Bioinformática. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Mesenchymal stem cells (MSCs) have been widely studied with regard to their potential use in cell therapy protocols and regenerative medicine. However, a better comprehension about the factors and molecular mechanisms driving cell differentiation is now mandatory to improve our chance to manipulate MSC behavior and to benefit future applications. In this work, we aimed to study gene regulatory networks at an early step of osteogenic differentiation. Therefore, we analyzed both the total mRNA and the mRNA fraction associated with polysomes on human adipose tissue-derived stem cells (hASCs) at 24 h of osteogenesis induction. The RNA-seq results evidenced that hASC fate is not compromised with osteogenesis at this time and that 21 days of continuous cell culture stimuli are necessary for full osteogenic differentiation of hASCs. Furthermore, early stages of osteogenesis induction involved gene regulation that was linked to the management of cell behavior in culture, such as the control of cell adhesion and proliferation. In conclusion, although discrete initial gene regulation related to osteogenesis occur, the first 24 h of induction is not sufficient to trigger and drive in vitro osteogenic differentiation of hASCs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/31797/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs41598-018-229.pdfs41598-018-229.pdfapplication/pdf1904124https://www.arca.fiocruz.br/bitstream/icict/31797/2/s41598-018-229.pdfd442ec2045dcbd7b53ef39c608c69343MD52TEXTs41598-018-229.pdf.txts41598-018-229.pdf.txtExtracted texttext/plain55337https://www.arca.fiocruz.br/bitstream/icict/31797/3/s41598-018-229.pdf.txtbac42848965a07a45d63a8d0b40ee360MD53icict/317972019-10-31 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| dc.title.pt_BR.fl_str_mv |
Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis |
| title |
Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis |
| spellingShingle |
Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis Robert, Anny Waloski Células-tronco mesenquimais Mesenchymal Stromal Cells Mesenchymal stem cells Osteogenesis Gene Expression Regulation Células del Estroma Mesenquimatoso Osteogénesis Regulación de la Expresión Génica Células Mesenquimais Estromais Osteogênese Regulação da Expressão Gênica |
| title_short |
Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis |
| title_full |
Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis |
| title_fullStr |
Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis |
| title_full_unstemmed |
Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis |
| title_sort |
Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis |
| author |
Robert, Anny Waloski |
| author_facet |
Robert, Anny Waloski Angulski, Addeli Bez Batti Spangenberg, Lucia Shigunov, Patrícia Pereira, Isabela Tiemy Bettes, Paulo Sergio Loiacono Naya, Hugo Dominguez, Alejandro Correa Dallagiovanna, Bruno Stimamiglio, Marco Augusto |
| author_role |
author |
| author2 |
Angulski, Addeli Bez Batti Spangenberg, Lucia Shigunov, Patrícia Pereira, Isabela Tiemy Bettes, Paulo Sergio Loiacono Naya, Hugo Dominguez, Alejandro Correa Dallagiovanna, Bruno Stimamiglio, Marco Augusto |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Robert, Anny Waloski Angulski, Addeli Bez Batti Spangenberg, Lucia Shigunov, Patrícia Pereira, Isabela Tiemy Bettes, Paulo Sergio Loiacono Naya, Hugo Dominguez, Alejandro Correa Dallagiovanna, Bruno Stimamiglio, Marco Augusto |
| dc.subject.other.pt_BR.fl_str_mv |
Células-tronco mesenquimais |
| topic |
Células-tronco mesenquimais Mesenchymal Stromal Cells Mesenchymal stem cells Osteogenesis Gene Expression Regulation Células del Estroma Mesenquimatoso Osteogénesis Regulación de la Expresión Génica Células Mesenquimais Estromais Osteogênese Regulação da Expressão Gênica |
| dc.subject.en.pt_BR.fl_str_mv |
Mesenchymal Stromal Cells Mesenchymal stem cells Osteogenesis Gene Expression Regulation |
| dc.subject.es.pt_BR.fl_str_mv |
Células del Estroma Mesenquimatoso Osteogénesis Regulación de la Expresión Génica |
| dc.subject.decs.pt_BR.fl_str_mv |
Células Mesenquimais Estromais Osteogênese Regulação da Expressão Gênica |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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2018 |
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2018 |
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2019-02-20T19:47:52Z |
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2019-02-20T19:47:52Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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ROBERT, Anny Waloski et al. Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis. Scientific Reports, v. 8, n. 1, p. 1-11, 2018. |
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https://www.arca.fiocruz.br/handle/icict/31797 |
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2045-2322 |
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10.1038/s41598-018-22991-6 |
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ROBERT, Anny Waloski et al. Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis. Scientific Reports, v. 8, n. 1, p. 1-11, 2018. 2045-2322 10.1038/s41598-018-22991-6 |
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Nature Research |
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Nature Research |
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