DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis

Detalhes bibliográficos
Autor(a) principal: Marcon, Bruna Hilzendeger
Data de Publicação: 2020
Outros Autores: Rebelatto, Carmen K., Cofré, Axel Helmut Rulf, Dallagiovanna, Bruno, Dominguez, Alejandro Correa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41328
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Marcon, Bruna HilzendegerRebelatto, Carmen K.Cofré, Axel Helmut RulfDallagiovanna, BrunoDominguez, Alejandro Correa2020-05-20T16:08:00Z2020-05-20T16:08:00Z2020MARCON, Bruna Hilzendeger et al. DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis. International Journal of Molecular Sciences. V.21, n.2607, p. 1-24, 2020.1422-0067https://www.arca.fiocruz.br/handle/icict/4132810.3390/ijms21072607porMDPIDDX6 helicaseMesenchymal Stem CellsRNA-Binding ProteinsAdipogenesisOsteogenesisCélulas Madre MesenquimatosasProteínas de Unión al ARNAdipogénesisOsteogénesisCélulas-Tronco MesenquimaisProteínas de Ligação a RNAAdipogeniaOsteogêneseDDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Universidade Federal do Alagoas. Laboratório de Inovação Farmacológica. Maceió, AL, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.DDX6 helicase is an RNA-binding protein involved in different aspects of gene expression regulation. The roles played by DDX6 depend on the complexes associated with it. Here, for the first time, we characterize the protein complexes associated with DDX6 in human adipose tissue-derived stem cells (hASCs) and analyze the dynamics of this helicase under different conditions of translational activity and differentiation. The results obtained demonstrated that the DDX6 helicase is associated with proteins involved in the control of mRNA localization, translation and metabolism in hASCs. DDX6 complexes may also assemble into more complex structures, such as RNA-dependent granules, the abundance and composition of which change upon inhibited translational activity. This finding supports the supposition that DDX6 is possibly involved in the regulation of the mRNA life cycle in hASCs. Although there was no significant variation in the protein composition of these complexes during early adipogenic or osteogenic induction, there was a change in the distribution pattern of DDX6: the number of DDX6 granules per cell was reduced during adipogenesis and was enhanced during osteogenesis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/41328/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALijms-21-0260.pdfijms-21-0260.pdfapplication/pdf7594828https://www.arca.fiocruz.br/bitstream/icict/41328/2/ijms-21-0260.pdf86dc631e0f9f3151eecf3d3cb81e6b13MD52TEXTijms-21-0260.pdf.txtijms-21-0260.pdf.txtExtracted texttext/plain91812https://www.arca.fiocruz.br/bitstream/icict/41328/3/ijms-21-0260.pdf.txtf83d07008aadee0241cd05471a4c45f9MD53icict/413282020-05-21 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dc.title.pt_BR.fl_str_mv DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis
title DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis
spellingShingle DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis
Marcon, Bruna Hilzendeger
DDX6 helicase
Mesenchymal Stem Cells
RNA-Binding Proteins
Adipogenesis
Osteogenesis
Células Madre Mesenquimatosas
Proteínas de Unión al ARN
Adipogénesis
Osteogénesis
Células-Tronco Mesenquimais
Proteínas de Ligação a RNA
Adipogenia
Osteogênese
title_short DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis
title_full DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis
title_fullStr DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis
title_full_unstemmed DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis
title_sort DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis
author Marcon, Bruna Hilzendeger
author_facet Marcon, Bruna Hilzendeger
Rebelatto, Carmen K.
Cofré, Axel Helmut Rulf
Dallagiovanna, Bruno
Dominguez, Alejandro Correa
author_role author
author2 Rebelatto, Carmen K.
Cofré, Axel Helmut Rulf
Dallagiovanna, Bruno
Dominguez, Alejandro Correa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Marcon, Bruna Hilzendeger
Rebelatto, Carmen K.
Cofré, Axel Helmut Rulf
Dallagiovanna, Bruno
Dominguez, Alejandro Correa
dc.subject.other.pt_BR.fl_str_mv DDX6 helicase
topic DDX6 helicase
Mesenchymal Stem Cells
RNA-Binding Proteins
Adipogenesis
Osteogenesis
Células Madre Mesenquimatosas
Proteínas de Unión al ARN
Adipogénesis
Osteogénesis
Células-Tronco Mesenquimais
Proteínas de Ligação a RNA
Adipogenia
Osteogênese
dc.subject.en.pt_BR.fl_str_mv Mesenchymal Stem Cells
RNA-Binding Proteins
Adipogenesis
Osteogenesis
dc.subject.es.pt_BR.fl_str_mv Células Madre Mesenquimatosas
Proteínas de Unión al ARN
Adipogénesis
Osteogénesis
dc.subject.decs.pt_BR.fl_str_mv Células-Tronco Mesenquimais
Proteínas de Ligação a RNA
Adipogenia
Osteogênese
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-05-20T16:08:00Z
dc.date.available.fl_str_mv 2020-05-20T16:08:00Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARCON, Bruna Hilzendeger et al. DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis. International Journal of Molecular Sciences. V.21, n.2607, p. 1-24, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41328
dc.identifier.issn.pt_BR.fl_str_mv 1422-0067
dc.identifier.doi.none.fl_str_mv 10.3390/ijms21072607
identifier_str_mv MARCON, Bruna Hilzendeger et al. DDX6 helicase behavior and protein partners in human adipose tissue-derived stem cells during early adipogenesis and osteogenesis. International Journal of Molecular Sciences. V.21, n.2607, p. 1-24, 2020.
1422-0067
10.3390/ijms21072607
url https://www.arca.fiocruz.br/handle/icict/41328
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/41328/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/41328/2/ijms-21-0260.pdf
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