Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function

Detalhes bibliográficos
Autor(a) principal: Wanderley, João Luiz Mendes
Data de Publicação: 2013
Outros Autores: Thorpe, Philip E., Barcinski, Marcelo André, Soong, Lynn
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11615
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Programa de Ciências Morfológicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Campus UFRJ Macaé. Pólo Universitário, Macaé, RJ, Brasil / University of Texas Medical Branch. Institute for Human Infections and Immunity. 3Department of Microbiology and Immunology. Galveston, TX, USA.
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spelling Wanderley, João Luiz MendesThorpe, Philip E.Barcinski, Marcelo AndréSoong, Lynn2015-08-19T13:49:41Z2015-08-19T13:49:41Z2013WANDERLEY, João Luiz Mendes; et al. Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function. Parasite Immunol., v.35, n.3-4, p.109–119, Mar. 2013.https://www.arca.fiocruz.br/handle/icict/1161510.1111/pim.12019engNIH Public AccessPhosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell functioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Programa de Ciências Morfológicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Campus UFRJ Macaé. Pólo Universitário, Macaé, RJ, Brasil / University of Texas Medical Branch. Institute for Human Infections and Immunity. 3Department of Microbiology and Immunology. Galveston, TX, USA.University of Texas Southwestern Medical Center. Department of Pharmacology. Dallas, TX, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil / Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia.São Paulo, SP, Brasil.University of Texas Southwestern Medical Center. Department of Pharmacology. Sealy Center for Vaccine Development. Center for Biodefense and Emerging Infectious Diseases. 7Department of Pathology. Dallas, TX, USA /Leishmania amazonensis parasites can cause diverse forms of leishmaniasis in humans and persistent lesions in most inbred strains of mice. In both cases, the infection is characterized by a marked immunosuppression of the host. We previously showed that amastigote forms of the parasite make use of surface-exposed phosphatidylserine (PS) molecules to infect host cells and promote alternative macrophage activation, leading to uncontrolled intracellular proliferation of the parasites. In this study, we demonstrated that treatment of infected mice with an PS-targeting monoclonal antibody ameliorated parasite loads and lesion development, which correlated with increased proliferative responses by lymphocytes. In addition, we observed an enhanced dendritic cell (DC) activation and antigen presentation in vitro. Our data imply that the recognition of PS exposed on the surface of amastigotes plays a role in down-modulating DC functions, in a matter similar to that of apoptotic cell clearance. This study provides new information regarding the mechanism of immune suppression in Leishmania infection.Leishmania amazonensisPhosphatidylserine (PS)Leishmania amazonensisCDPdiacilglicerol-Serina O-Fosfatidiltransferaseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11615/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmarcelo_marcinski2_etal_IOC-2013.pdfapplication/pdf2808095https://www.arca.fiocruz.br/bitstream/icict/11615/2/marcelo_marcinski2_etal_IOC-2013.pdfedcdb98bfead0b5ab32e449b0789faf4MD52TEXTmarcelo_marcinski2_etal_IOC-2013.pdf.txtmarcelo_marcinski2_etal_IOC-2013.pdf.txtExtracted texttext/plain50227https://www.arca.fiocruz.br/bitstream/icict/11615/3/marcelo_marcinski2_etal_IOC-2013.pdf.txta0546e94f6481924288902b8a8a1d7a5MD53icict/116152022-06-24 13:12:38.436oai:www.arca.fiocruz.br:icict/11615TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:12:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function
title Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function
spellingShingle Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function
Wanderley, João Luiz Mendes
Leishmania amazonensis
Phosphatidylserine (PS)
Leishmania amazonensis
CDPdiacilglicerol-Serina O-Fosfatidiltransferase
title_short Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function
title_full Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function
title_fullStr Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function
title_full_unstemmed Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function
title_sort Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function
author Wanderley, João Luiz Mendes
author_facet Wanderley, João Luiz Mendes
Thorpe, Philip E.
Barcinski, Marcelo André
Soong, Lynn
author_role author
author2 Thorpe, Philip E.
Barcinski, Marcelo André
Soong, Lynn
author2_role author
author
author
dc.contributor.author.fl_str_mv Wanderley, João Luiz Mendes
Thorpe, Philip E.
Barcinski, Marcelo André
Soong, Lynn
dc.subject.en.pt_BR.fl_str_mv Leishmania amazonensis
Phosphatidylserine (PS)
topic Leishmania amazonensis
Phosphatidylserine (PS)
Leishmania amazonensis
CDPdiacilglicerol-Serina O-Fosfatidiltransferase
dc.subject.decs.pt_BR.fl_str_mv Leishmania amazonensis
CDPdiacilglicerol-Serina O-Fosfatidiltransferase
description Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Programa de Ciências Morfológicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Campus UFRJ Macaé. Pólo Universitário, Macaé, RJ, Brasil / University of Texas Medical Branch. Institute for Human Infections and Immunity. 3Department of Microbiology and Immunology. Galveston, TX, USA.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:41Z
dc.date.available.fl_str_mv 2015-08-19T13:49:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WANDERLEY, João Luiz Mendes; et al. Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function. Parasite Immunol., v.35, n.3-4, p.109–119, Mar. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11615
dc.identifier.doi.pt_BR.fl_str_mv 10.1111/pim.12019
identifier_str_mv WANDERLEY, João Luiz Mendes; et al. Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function. Parasite Immunol., v.35, n.3-4, p.109–119, Mar. 2013.
10.1111/pim.12019
url https://www.arca.fiocruz.br/handle/icict/11615
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv NIH Public Access
publisher.none.fl_str_mv NIH Public Access
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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