Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization

Detalhes bibliográficos
Autor(a) principal: Rübsamen-Waigmann, H
Data de Publicação: 1994
Outros Autores: von Briesen, H, Holmes, H, Björndal, A, Korber, B, Esser, R, Ranjbar, S, Tomlinson, P, Castro Filho, Bernardo Galvão, Karita, E, Sempala, S, Wasi, C, Osmanov, S, Fenyo, E.-M, WHO Network for HIV Isolation and Characterization, WHO Network
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15222
Resumo: Georg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, Germany
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spelling Rübsamen-Waigmann, Hvon Briesen, HHolmes, HBjörndal, AKorber, BEsser, RRanjbar, STomlinson, PCastro Filho, Bernardo GalvãoKarita, ESempala, SWasi, COsmanov, SFenyo, E.-MWHO Network for HIV Isolation and Characterization, WHO Network2016-08-10T13:12:37Z2016-08-10T13:12:37Z1994RUBSAMEN-WAIGMANN, H. et al. Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization. AIDS Research and Human Retroviruses, v. 10, n. 11, p. 1401-1408, 1994.0889-2229https://www.arca.fiocruz.br/handle/icict/15222engMary Ann LiebertStandard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGeorg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, GermanyGeorg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, GermanyNational Institute of Biological Standards and Control. London, UKLos Alamos National Laboratory. HIV Sequence Database. Los Alamos, New MexicoGeorg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, GermanyNational Institute of Biological Standards and Control. London, UKNational Institute of Biological Standards and Control. London, UKFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitute of Tropical Medicine. Department of Microbiology. Antwerp, BelgiumUganda Virus Research Institute. Entebbe, UgandaMahidol University. Faculty of Medicine Siriraj Hospital. Department of Microbiology, Division of Virology. Bangkok, ThailandWorld Health Organization. Global Programme on AIDS. Geneva, SwitzerlandLos Alamos National Laboratory. HIV Sequence Database. Los Alamos, New MexicoWHO Network for HIV Isolation and CharacterizationHIV-1 isolates were obtained from four countries within the framework of the WHO Network for HIV Isolation and Characterization. The use of standard HIV isolation procedures allowed us to compare the biological properties of 126 HIV-1 isolates spanning five genetic subtypes. In primary isolation cultures, viruses from Uganda and Brazil appeared early and replicated without delay, whereas the replication of Thai viruses was delayed by several weeks. Regardless of genetic subtype or country of origin, blood samples collected more than 2 years after seroconversion yielded virus that replicated efficiently in the primary isolation cultures. None of the isolates obtained from Thailand or Rwanda replicated in cell lines, whereas 5 of the 13 Brazilian isolates and 7 of the 11 Ugandan isolates replicated and induced syncytia in Ml -2 cells. As expected for virus isolates obtained early in HIV-1 infection (within 2 years of seroconversion), all viruses from Brazil, Rwanda, and Thailand showed a slow/low replicative pattern. For the Ugandan samples, the time from seroconversion was known precisely for a few of the samples and only in one case was less than 2 years. This may explain why the five viruses that were able to replicate in all cell lines, and thus classified as rapid/high, were of Ugandan origin. Viruses able to induce syncytia in MT-2 cells, also induced syncytia in PBMC. However, 8 slow/low viruses (out of 27) gave discordant results, inducing syncytia in PBMC but not in MT-2 cells. Furthermore, using syncytium induction as a marker, changes in virus populations during early in vitro passage in PBMC could be observed. The results indicate that biological variation is a general property of HIV-1 in different regions of the world. Moreover, the time from HIV-1 infection, rather than genetic subtype, seems to be linked to viral phenotype.HIV-1HIV InfectionsLeukocytes, MononuclearPhenotypeVirus ReplicationWorld Health OrganizationGenetic VariationGenotypeCytopathogenic Effect, ViralBrazilHumansinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15222/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRubsamen-Waigmann H Standard conditions....pdfRubsamen-Waigmann H Standard conditions....pdfapplication/pdf1168798https://www.arca.fiocruz.br/bitstream/icict/15222/2/Rubsamen-Waigmann%20H%20Standard%20conditions....pdf8a27aca198229a64007a9bbceb037d23MD52TEXTRubsamen-Waigmann H Standard conditions....pdf.txtRubsamen-Waigmann H Standard conditions....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization
title Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization
spellingShingle Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization
Rübsamen-Waigmann, H
HIV-1
HIV Infections
Leukocytes, Mononuclear
Phenotype
Virus Replication
World Health Organization
Genetic Variation
Genotype
Cytopathogenic Effect, Viral
Brazil
Humans
title_short Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization
title_full Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization
title_fullStr Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization
title_full_unstemmed Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization
title_sort Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization
author Rübsamen-Waigmann, H
author_facet Rübsamen-Waigmann, H
von Briesen, H
Holmes, H
Björndal, A
Korber, B
Esser, R
Ranjbar, S
Tomlinson, P
Castro Filho, Bernardo Galvão
Karita, E
Sempala, S
Wasi, C
Osmanov, S
Fenyo, E.-M
WHO Network for HIV Isolation and Characterization, WHO Network
author_role author
author2 von Briesen, H
Holmes, H
Björndal, A
Korber, B
Esser, R
Ranjbar, S
Tomlinson, P
Castro Filho, Bernardo Galvão
Karita, E
Sempala, S
Wasi, C
Osmanov, S
Fenyo, E.-M
WHO Network for HIV Isolation and Characterization, WHO Network
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rübsamen-Waigmann, H
von Briesen, H
Holmes, H
Björndal, A
Korber, B
Esser, R
Ranjbar, S
Tomlinson, P
Castro Filho, Bernardo Galvão
Karita, E
Sempala, S
Wasi, C
Osmanov, S
Fenyo, E.-M
WHO Network for HIV Isolation and Characterization, WHO Network
dc.subject.en.pt_BR.fl_str_mv HIV-1
HIV Infections
Leukocytes, Mononuclear
Phenotype
Virus Replication
World Health Organization
Genetic Variation
Genotype
Cytopathogenic Effect, Viral
Brazil
Humans
topic HIV-1
HIV Infections
Leukocytes, Mononuclear
Phenotype
Virus Replication
World Health Organization
Genetic Variation
Genotype
Cytopathogenic Effect, Viral
Brazil
Humans
description Georg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, Germany
publishDate 1994
dc.date.issued.fl_str_mv 1994
dc.date.accessioned.fl_str_mv 2016-08-10T13:12:37Z
dc.date.available.fl_str_mv 2016-08-10T13:12:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv RUBSAMEN-WAIGMANN, H. et al. Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization. AIDS Research and Human Retroviruses, v. 10, n. 11, p. 1401-1408, 1994.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15222
dc.identifier.issn.pt_BR.fl_str_mv 0889-2229
identifier_str_mv RUBSAMEN-WAIGMANN, H. et al. Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization. AIDS Research and Human Retroviruses, v. 10, n. 11, p. 1401-1408, 1994.
0889-2229
url https://www.arca.fiocruz.br/handle/icict/15222
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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