Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease

Detalhes bibliográficos
Autor(a) principal: Torrentes-Carvalho, Amanda
Data de Publicação: 2020
Outros Autores: Sánchez-Arcila, Juan Camilo, Azamor, Tamiris, Barbosa, Luciana Santos, Hottz, Eugênio Damaceno, Gandini, Mariana, Bozza, Fernando Augusto, Cunha, Rivaldo Venâncio da, Pinto, Luzia Maria de Oliveira, Damasco, Paulo Vieira, Azeredo, Elzinandes Leal de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40932
Resumo: Universidade Federal Fluminense. Instituto de Biologia. Departamento de Imunobiologia. Niterói, RJ, Brasil.
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spelling Torrentes-Carvalho, AmandaSánchez-Arcila, Juan CamiloAzamor, TamirisBarbosa, Luciana SantosHottz, Eugênio DamacenoGandini, MarianaBozza, Fernando AugustoCunha, Rivaldo Venâncio daPinto, Luzia Maria de OliveiraDamasco, Paulo VieiraAzeredo, Elzinandes Leal de2020-04-23T13:49:29Z2020-04-23T13:49:29Z2020TORRENTES-CARVALHO, Amanda et al. Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease. Scientific Reports, v. 10, n. 6351, p. 1-13, 14 Apr. 2020.2045-2322https://www.arca.fiocruz.br/handle/icict/4093210.1038/s41598-020-62776-4engNature ResearchApoptoseLeucócitos de sangue mononuclearPacientes com HIVDengue agudaApoptosisMononuclear blood leukocytesHIV patientsDengue acute diseaseApoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Instituto de Biologia. Departamento de Imunobiologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil.Universidade Federal do Mato Grosso do Sul. Faculdade de Medicina. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gafreé & Guinle. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Rio de Janeiro. Hospital Pedro Ernesto. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Dengue virus (DENV) co-circulation in Brazil represents a challenge for treatment and vaccine development. Despite public health impact, the occurrence of coinfections with other viruses is a common event. Increased T cell activation and altered inflammatory response are found during DENV coinfection with Human Immunodeficiency Virus (HIV) impacting HIV-pathogenesis. Even with Antiretroviral therapy (ART), HIV- treated patients had chronic immune activation and lymphocyte apoptosis. However, apoptotic mechanisms have not been investigated during coinfection with DENV. Our attention was attracted to apoptotic cell markers expressions in PBMCs from DENV and DENV/HIV coinfected patients. We found CD4/CD8 ratio inversion in most coinfected patients. CD4 T and CD8 T-cell subsets from DENV and DENV/HIV groups expressed low levels of anti-apoptotic protein Bcl-2. Furthermore, CD8 CD95 double positive cells frequency expressing low levels of Bcl-2 were significantly higher in these patients. Additionally, the density of Bcl-2 on classical monocytes (CD14++CD16-) was significantly lower during DENV infection. Upregulation of pro-apoptotic proteins and anti-apoptotic proteins were found in DENV and DENV/HIV, while catalase, an antioxidant protein, was upregulated mainly in DENV/HIV coinfection. These findings provide evidence of apoptosis triggering during DENV/HIV coinfection, which may contribute to knowledge of immunological response during DENV acute infection in HIV-patients treated with ART.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40932/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALElzinandesLAzeredo_LucianaBarbosa_etal_IOC_2020.pdfElzinandesLAzeredo_LucianaBarbosa_etal_IOC_2020.pdfapplication/pdf1881180https://www.arca.fiocruz.br/bitstream/icict/40932/2/ElzinandesLAzeredo_LucianaBarbosa_etal_IOC_2020.pdf2708273f5170fef9eb7d8a5396ee7013MD52TEXTElzinandesLAzeredo_LucianaBarbosa_etal_IOC_2020.pdf.txtElzinandesLAzeredo_LucianaBarbosa_etal_IOC_2020.pdf.txtExtracted 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dc.title.en.fl_str_mv Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
title Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
spellingShingle Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
Torrentes-Carvalho, Amanda
Apoptose
Leucócitos de sangue mononuclear
Pacientes com HIV
Dengue aguda
Apoptosis
Mononuclear blood leukocytes
HIV patients
Dengue acute disease
title_short Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
title_full Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
title_fullStr Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
title_full_unstemmed Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
title_sort Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease
author Torrentes-Carvalho, Amanda
author_facet Torrentes-Carvalho, Amanda
Sánchez-Arcila, Juan Camilo
Azamor, Tamiris
Barbosa, Luciana Santos
Hottz, Eugênio Damaceno
Gandini, Mariana
Bozza, Fernando Augusto
Cunha, Rivaldo Venâncio da
Pinto, Luzia Maria de Oliveira
Damasco, Paulo Vieira
Azeredo, Elzinandes Leal de
author_role author
author2 Sánchez-Arcila, Juan Camilo
Azamor, Tamiris
Barbosa, Luciana Santos
Hottz, Eugênio Damaceno
Gandini, Mariana
Bozza, Fernando Augusto
Cunha, Rivaldo Venâncio da
Pinto, Luzia Maria de Oliveira
Damasco, Paulo Vieira
Azeredo, Elzinandes Leal de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Torrentes-Carvalho, Amanda
Sánchez-Arcila, Juan Camilo
Azamor, Tamiris
Barbosa, Luciana Santos
Hottz, Eugênio Damaceno
Gandini, Mariana
Bozza, Fernando Augusto
Cunha, Rivaldo Venâncio da
Pinto, Luzia Maria de Oliveira
Damasco, Paulo Vieira
Azeredo, Elzinandes Leal de
dc.subject.other.pt_BR.fl_str_mv Apoptose
Leucócitos de sangue mononuclear
Pacientes com HIV
Dengue aguda
topic Apoptose
Leucócitos de sangue mononuclear
Pacientes com HIV
Dengue aguda
Apoptosis
Mononuclear blood leukocytes
HIV patients
Dengue acute disease
dc.subject.en.pt_BR.fl_str_mv Apoptosis
Mononuclear blood leukocytes
HIV patients
Dengue acute disease
description Universidade Federal Fluminense. Instituto de Biologia. Departamento de Imunobiologia. Niterói, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-04-23T13:49:29Z
dc.date.available.fl_str_mv 2020-04-23T13:49:29Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TORRENTES-CARVALHO, Amanda et al. Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease. Scientific Reports, v. 10, n. 6351, p. 1-13, 14 Apr. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40932
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.pt_BR.fl_str_mv 10.1038/s41598-020-62776-4
identifier_str_mv TORRENTES-CARVALHO, Amanda et al. Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease. Scientific Reports, v. 10, n. 6351, p. 1-13, 14 Apr. 2020.
2045-2322
10.1038/s41598-020-62776-4
url https://www.arca.fiocruz.br/handle/icict/40932
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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