Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations

Detalhes bibliográficos
Autor(a) principal: Britto, Constança Carvalho
Data de Publicação: 2009
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27122
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.
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spelling Britto, Constança Carvalho2018-06-26T16:54:55Z2018-06-26T16:54:55Z2009BRITTO, Constança Carvalho. Mem Inst Oswaldo Cruz, v..104, p.122-135, Suppl. I, 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/271221678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzDoença de Chagasdiagnóstico molecularTrypanosoma cruziQuimioterapiaavaliação de curaChagas DiseasechemotherapyTrypanosoma cruzimolecular diagnosiscure assessmentUsefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.One major goal of research on Chagas disease is the development of effective chemotherapy to eliminate the infection from individuals who have not yet developed cardiac and/or digestive disease manifestations. Cure evaluation is the more complex aspect of its treatment, often leading to diverse and controversial results. The absence of reliable methods or a diagnostic gold standard to assess etiologic treatment efficacy still constitutes a major challenge. In an effort to develop more sensitive tools, polymerase chain reaction (PCR)-based assays were introduced to detect low amounts of Trypanosoma cruzi DNA in blood samples from chagasic patients, thus improving the diagnosis and follow-up evaluation after chemotherapy. In this article, I review the main problems concerning drug efficacy and criteria used for cure estimation in treated chagasic patients, and the work conducted by different groups on developing PCR methodologies to monitor treatment outcome of congenital infections as well as recent and late chronic T. cruzi infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27122/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALconstança_brito_IOC_2009.pdfconstança_brito_IOC_2009.pdfapplication/pdf845972https://www.arca.fiocruz.br/bitstream/icict/27122/2/constan%c3%a7a_brito_IOC_2009.pdf736575c0425bab79be9ac741a29074e3MD52TEXTconstança_brito_IOC_2009.pdf.txtconstança_brito_IOC_2009.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
title Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
spellingShingle Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
Britto, Constança Carvalho
Doença de Chagas
diagnóstico molecular
Trypanosoma cruzi
Quimioterapia
avaliação de cura
Chagas Disease
chemotherapy
Trypanosoma cruzi
molecular diagnosis
cure assessment
title_short Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
title_full Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
title_fullStr Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
title_full_unstemmed Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
title_sort Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
author Britto, Constança Carvalho
author_facet Britto, Constança Carvalho
author_role author
dc.contributor.author.fl_str_mv Britto, Constança Carvalho
dc.subject.other.pt_BR.fl_str_mv Doença de Chagas
diagnóstico molecular
Trypanosoma cruzi
Quimioterapia
avaliação de cura
topic Doença de Chagas
diagnóstico molecular
Trypanosoma cruzi
Quimioterapia
avaliação de cura
Chagas Disease
chemotherapy
Trypanosoma cruzi
molecular diagnosis
cure assessment
dc.subject.en.pt_BR.fl_str_mv Chagas Disease
chemotherapy
Trypanosoma cruzi
molecular diagnosis
cure assessment
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-06-26T16:54:55Z
dc.date.available.fl_str_mv 2018-06-26T16:54:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRITTO, Constança Carvalho. Mem Inst Oswaldo Cruz, v..104, p.122-135, Suppl. I, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27122
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BRITTO, Constança Carvalho. Mem Inst Oswaldo Cruz, v..104, p.122-135, Suppl. I, 2009.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/27122
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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