Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut

Detalhes bibliográficos
Autor(a) principal: Uehara, Lívia Almeida
Data de Publicação: 2012
Outros Autores: Moreira, Otacilio C., Oliveira, Ana Carolina, Azambuja, Patricia, Lima, Ana Paula Cabral Araujo, Britto, Constança, Santos, André Luis Souza dos, Branquinha, Marta Helena, Levy, Claudia Masini d'Avila
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16226
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
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spelling Uehara, Lívia AlmeidaMoreira, Otacilio C.Oliveira, Ana CarolinaAzambuja, PatriciaLima, Ana Paula Cabral AraujoBritto, ConstançaSantos, André Luis Souza dosBranquinha, Marta HelenaLevy, Claudia Masini d'Avila2016-10-13T17:07:57Z2016-10-13T17:07:57Z2012UEHARA, Lívia; et al. Cruzipain Promotes Trypanosoma cruzi Adhesion to Rhodnius prolixus Midgut. PLoS Negl Trop Dis., v.6, n.12, e1958, 11p, Dec. 2012.1935-2727https://www.arca.fiocruz.br/handle/icict/1622610.1371/journal.pntd.00019581935-2735engPublic Library of ScienceTrypanosoma cruziRhodnius prolixusDoença de ChagasCruzipaínaTrypanosoma cruziRhodnius prolixusCruzipainChagas DiseaseCruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgutinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil / Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Bioquímica e Biologia Molecular de Proteases. Rio de janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Departamento de Microbiologia Celular. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Departamento de Microbiologia Celular. Laboratório de Estudos Integrados em Bioquímica de Proteases. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Background: Trypanosoma cruzi is the etiological agent of Chagas’ disease. Cysteine peptidases are relevant to several aspects of the T. cruzi life cycle and are implicated in parasite-mammalian host relationships. However, little is known about the factors that contribute to the parasite-insect host interaction. Methodology/Principal Findings: Here, we have investigated whether cruzipain could be involved in the interaction of T. cruzi with the invertebrate host. We analyzed the effect of treatment of T. cruzi epimastigotes with anti-cruzipain antibodies or with a panel of cysteine peptidase inhibitors (cystatin, antipain, E-64, leupeptin, iodocetamide or CA-074-OMe) on parasite adhesion to Rhodnius prolixus posterior midgut ex vivo. All treatments, with the exception of CA074-OMe, significantly decreased parasite adhesion to R. prolixus midgut. Cystatin presented a dose-dependent reduction on the adhesion. Comparison of the adhesion rate among several T. cruzi isolates revealed that the G isolate, which naturally possesses low levels of active cruzipain, adhered to a lesser extent in comparison to Dm28c, Y and CL Brener isolates. Transgenic epimastigotes overexpressing an endogenous cruzipain inhibitor (pCHAG), chagasin, and that have reduced levels of active cruzipain adhered to the insect gut 73% less than the wild-type parasites. The adhesion of pCHAG parasites was partially restored by the addition of exogenous cruzipain. In vivo colonization experiments revealed low levels of pCHAG parasites in comparison to wild-type. Parasites isolated after passage in the insect presented a drastic enhancement in the expression of surface cruzipain. Conclusions/Significance: These data highlight, for the first time, that cruzipain contributes to the interaction of T. cruzi with the insect host.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16226/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALlivia_uehara_etal_IOC_2012.pdflivia_uehara_etal_IOC_2012.pdfapplication/pdf574657https://www.arca.fiocruz.br/bitstream/icict/16226/2/livia_uehara_etal_IOC_2012.pdf001227bfb1811283a09795b5e95f7acfMD52TEXTlivia_uehara_etal_IOC_2012.pdf.txtlivia_uehara_etal_IOC_2012.pdf.txtExtracted texttext/plain56729https://www.arca.fiocruz.br/bitstream/icict/16226/3/livia_uehara_etal_IOC_2012.pdf.txtecd95abf1b4f9c866ca49ee0dc9fefb7MD53icict/162262023-09-05 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dc.title.pt_BR.fl_str_mv Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
title Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
spellingShingle Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
Uehara, Lívia Almeida
Trypanosoma cruzi
Rhodnius prolixus
Doença de Chagas
Cruzipaína
Trypanosoma cruzi
Rhodnius prolixus
Cruzipain
Chagas Disease
title_short Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
title_full Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
title_fullStr Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
title_full_unstemmed Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
title_sort Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
author Uehara, Lívia Almeida
author_facet Uehara, Lívia Almeida
Moreira, Otacilio C.
Oliveira, Ana Carolina
Azambuja, Patricia
Lima, Ana Paula Cabral Araujo
Britto, Constança
Santos, André Luis Souza dos
Branquinha, Marta Helena
Levy, Claudia Masini d'Avila
author_role author
author2 Moreira, Otacilio C.
Oliveira, Ana Carolina
Azambuja, Patricia
Lima, Ana Paula Cabral Araujo
Britto, Constança
Santos, André Luis Souza dos
Branquinha, Marta Helena
Levy, Claudia Masini d'Avila
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Uehara, Lívia Almeida
Moreira, Otacilio C.
Oliveira, Ana Carolina
Azambuja, Patricia
Lima, Ana Paula Cabral Araujo
Britto, Constança
Santos, André Luis Souza dos
Branquinha, Marta Helena
Levy, Claudia Masini d'Avila
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Rhodnius prolixus
Doença de Chagas
Cruzipaína
topic Trypanosoma cruzi
Rhodnius prolixus
Doença de Chagas
Cruzipaína
Trypanosoma cruzi
Rhodnius prolixus
Cruzipain
Chagas Disease
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Rhodnius prolixus
Cruzipain
Chagas Disease
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-10-13T17:07:57Z
dc.date.available.fl_str_mv 2016-10-13T17:07:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv UEHARA, Lívia; et al. Cruzipain Promotes Trypanosoma cruzi Adhesion to Rhodnius prolixus Midgut. PLoS Negl Trop Dis., v.6, n.12, e1958, 11p, Dec. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16226
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001958
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv UEHARA, Lívia; et al. Cruzipain Promotes Trypanosoma cruzi Adhesion to Rhodnius prolixus Midgut. PLoS Negl Trop Dis., v.6, n.12, e1958, 11p, Dec. 2012.
1935-2727
10.1371/journal.pntd.0001958
1935-2735
url https://www.arca.fiocruz.br/handle/icict/16226
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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