Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection
| Autor(a) principal: | |
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| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19893 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Universidade Federal Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. |
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Nogueira, Paula MonalisaAssis, Rafael RamiroTorrecilhas, Ana Claudia TrocoliSaraiva, Elvira MariaPessoa, Natália LimaCampos, Marco AntonioSantos, Eric Fabricio Marialva dosRíos-Velasquez, Cláudia MariaPessoa, Felipe A.Secundino, Nágila Francinete CostaRugani, Jeronimo Marteleto NunesNieves, ElsaTurco, Salvatore J.Melo, Maria NormaSoares, Rodrigo Pedro Pinto2017-07-10T17:21:18Z2017-07-10T17:21:18Z2016NOGUEIRA, Paula Monalisa et al. Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection. PLoS Neglected Tropical Diseases, v. 10, n. 8, p. 1-17, 2016.1935-2727https://www.arca.fiocruz.br/handle/icict/1989310.1371/journal.pntd.0004848porPublic Library of ScienceLeishmaniosesLeishmaniose cutaneaLeishmaniasisCutaneous LeishmaniasisLipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Universidade Federal Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Departamento de Ciências Biológicas. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, São Paulo, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Imunologia. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidad de Los Andes. Departamento de Biologia. Laboratório de Parasitologia Experimental. Mérida, Venezuela.University of Kentucky Medical Center. Department of Biochemistry. Lexington, Kentucky, USA / Liverpool School of Tropical Medicine, United Kingdom.Universidade Federal Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.The immunomodulatory properties of lipophosphoglycans (LPG) from New World species of Leishmania have been assessed in Leishmania infantum and Leishmania braziliensis, the causative agents of visceral and cutaneous leishmaniasis, respectively. This glycoconjugate is highly polymorphic among species with variation in sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Here, the immunomodulatory activity of LPGs from Leishmania amazonensis, the causative agent of diffuse cutaneous leishmaniasis, was evaluated in two strains from Brazil. One strain (PH8) was originally isolated from the sand fly and the other (Josefa) was isolated from a human case. The ability of purified LPGs from both strains was investigated during in vitro interaction with peritoneal murine macrophages and CHO cells and in vivo infection with Lutzomyia migonei. In peritoneal murine macrophages, the LPGs from both strains activated TLR4. Both LPGs equally activate MAPKs and the NF-κB inhibitor p-IκBα, but were not able to translocate NF-κB. In vivo experiments with sand flies showed that both stains were able to sustain infection in L. migonei. A preliminary biochemical analysis indicates intraspecies variation in the LPG sugar moieties. However, they did not result in different activation profiles of the innate immune system. Also those polymorphisms did not affect infectivity to the sand fly.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/19893/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALve_Nogueira_Paula_Lipophosphoglycans_IRR_2016.pdfve_Nogueira_Paula_Lipophosphoglycans_IRR_2016.pdfapplication/pdf6574205https://www.arca.fiocruz.br/bitstream/icict/19893/2/ve_Nogueira_Paula_Lipophosphoglycans_IRR_2016.pdf6e49e5f2e6d83c0ed3041eae86cdbdddMD52TEXTve_Nogueira_Paula_Lipophosphoglycans_IRR_2016.pdf.txtve_Nogueira_Paula_Lipophosphoglycans_IRR_2016.pdf.txtExtracted texttext/plain734https://www.arca.fiocruz.br/bitstream/icict/19893/3/ve_Nogueira_Paula_Lipophosphoglycans_IRR_2016.pdf.txt29133a82ce0c8fb99452fb1eab272dd4MD53icict/198932019-08-27 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| dc.title.pt_BR.fl_str_mv |
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection |
| title |
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection |
| spellingShingle |
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection Nogueira, Paula Monalisa Leishmanioses Leishmaniose cutanea Leishmaniasis Cutaneous Leishmaniasis |
| title_short |
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection |
| title_full |
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection |
| title_fullStr |
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection |
| title_full_unstemmed |
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection |
| title_sort |
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection |
| author |
Nogueira, Paula Monalisa |
| author_facet |
Nogueira, Paula Monalisa Assis, Rafael Ramiro Torrecilhas, Ana Claudia Trocoli Saraiva, Elvira Maria Pessoa, Natália Lima Campos, Marco Antonio Santos, Eric Fabricio Marialva dos Ríos-Velasquez, Cláudia Maria Pessoa, Felipe A. Secundino, Nágila Francinete Costa Rugani, Jeronimo Marteleto Nunes Nieves, Elsa Turco, Salvatore J. Melo, Maria Norma Soares, Rodrigo Pedro Pinto |
| author_role |
author |
| author2 |
Assis, Rafael Ramiro Torrecilhas, Ana Claudia Trocoli Saraiva, Elvira Maria Pessoa, Natália Lima Campos, Marco Antonio Santos, Eric Fabricio Marialva dos Ríos-Velasquez, Cláudia Maria Pessoa, Felipe A. Secundino, Nágila Francinete Costa Rugani, Jeronimo Marteleto Nunes Nieves, Elsa Turco, Salvatore J. Melo, Maria Norma Soares, Rodrigo Pedro Pinto |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nogueira, Paula Monalisa Assis, Rafael Ramiro Torrecilhas, Ana Claudia Trocoli Saraiva, Elvira Maria Pessoa, Natália Lima Campos, Marco Antonio Santos, Eric Fabricio Marialva dos Ríos-Velasquez, Cláudia Maria Pessoa, Felipe A. Secundino, Nágila Francinete Costa Rugani, Jeronimo Marteleto Nunes Nieves, Elsa Turco, Salvatore J. Melo, Maria Norma Soares, Rodrigo Pedro Pinto |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmanioses Leishmaniose cutanea |
| topic |
Leishmanioses Leishmaniose cutanea Leishmaniasis Cutaneous Leishmaniasis |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmaniasis Cutaneous Leishmaniasis |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Universidade Federal Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. |
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2016 |
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2016 |
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2017-07-10T17:21:18Z |
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2017-07-10T17:21:18Z |
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NOGUEIRA, Paula Monalisa et al. Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection. PLoS Neglected Tropical Diseases, v. 10, n. 8, p. 1-17, 2016. |
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https://www.arca.fiocruz.br/handle/icict/19893 |
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1935-2727 |
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10.1371/journal.pntd.0004848 |
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NOGUEIRA, Paula Monalisa et al. Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection. PLoS Neglected Tropical Diseases, v. 10, n. 8, p. 1-17, 2016. 1935-2727 10.1371/journal.pntd.0004848 |
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