Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice

Detalhes bibliográficos
Autor(a) principal: Meloni-Bruneri, L. H.
Data de Publicação: 1996
Outros Autores: Campa, A., Abdalla, D. S., Calich, Vera Lucia G., Lenzi, Henrique Leonel, Burger, Eva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3838
Resumo: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.
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spelling Meloni-Bruneri, L. H.Campa, A.Abdalla, D. S.Calich, Vera Lucia G.Lenzi, Henrique LeonelBurger, Eva2012-04-13T18:50:41Z2012-04-13T18:50:41Z1996MELONI-BRUNERI, L. H. et al. Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice. Journal of Leukocyte Biology, v. 59, n. 4, p. 526-33, Apr. 1996.0741-5400https://www.arca.fiocruz.br/handle/icict/3838engSociety for Leukocyte BiologyNeutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Médicas. Departamento de Imunologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.niversidade de São Paulo. Instituto de Ciências Médicas. Departamento de Imunologia. São Paulo, SP, Brasil.The oxidative burst of polymorphonuclear neutrophils (PMN) and their ability to inhibit Paracoccidioides brasiliensis growth was studied in susceptible (B10.A) and resistant (A/J) mice. The cells were obtained after subcutaneous inoculation in air pouches, yielding highly pure PMN preparations; the number of cells was similar for both strains at 24 h and five times higher in the resistant strain at 15 days. The oxidative metabolism of these PMN was evaluated by the luminol and lucigen-enhanced chemiluminescence upon stimulation with PMA or killed P. brasiliensis (Pb). At 24 h of infection PMN from both strains showed similar responses. However, at 15 days a great enhancement of the Pb-stimulated luminol-enhanced chemiluminescence was observed only in PMN from resistant mice. Such increase was markedly inhibited by the addition of catalase. Independent of the mouse strain or time of infection of lucigen-enhanced chemiluminescence showed the same intensity. The lucigen-enhanced chemiluminescence of PMN without stimuli from resistant mice did not change with the time of infection, however, after 15 days of infection a significantly lower chemiluminescence was detected with PMN from susceptible mice. At 15 days of infection the PMN from B10.A were unable to kill P. brasiliensis yeast cells in vitro. Because the lucigenin- and luminol-enhanced chemiluminescence detects, respectively, the O2- production and the myeloperoxidase/hydrogen peroxide halide system, the present data show parallels between deficiency in the production of oxygen-reactive species by PMN and lower fungicidal activity.Disease SusceptibilityLuminescent MeasurementsLuminolNeutrophilsParacoccidioidomycosisParacoccidioidesSuscetibilidade a DoençasMedições LuminescentesLuminolNeutrófilosParacoccidioidomicoseParacoccidioidesImunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNeutrophil oxidative metabolism and killing of P. brasiliensis.pdfNeutrophil oxidative metabolism and killing of P. brasiliensis.pdfapplication/pdf1480678https://www.arca.fiocruz.br/bitstream/icict/3838/1/Neutrophil%20oxidative%20metabolism%20and%20killing%20of%20P.%20brasiliensis.pdf7891f1385e602ed5c1bdab5dd042531eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3838/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTNeutrophil oxidative metabolism and killing of P. brasiliensis.pdf.txtNeutrophil oxidative metabolism and killing of P. brasiliensis.pdf.txtExtracted texttext/plain38378https://www.arca.fiocruz.br/bitstream/icict/3838/5/Neutrophil%20oxidative%20metabolism%20and%20killing%20of%20P.%20brasiliensis.pdf.txt70b353f4d18698c75b0a14b2e96a62fdMD55THUMBNAILNeutrophil oxidative metabolism and killing of P. brasiliensis.pdf.jpgNeutrophil oxidative metabolism and killing of P. brasiliensis.pdf.jpgGenerated Thumbnailimage/jpeg2909https://www.arca.fiocruz.br/bitstream/icict/3838/4/Neutrophil%20oxidative%20metabolism%20and%20killing%20of%20P.%20brasiliensis.pdf.jpge04029abc8f15e9556483d481fe0a04aMD54icict/38382018-04-06 09:06:41.883oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T12:06:41Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice
title Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice
spellingShingle Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice
Meloni-Bruneri, L. H.
Disease Susceptibility
Luminescent Measurements
Luminol
Neutrophils
Paracoccidioidomycosis
Paracoccidioides
Suscetibilidade a Doenças
Medições Luminescentes
Luminol
Neutrófilos
Paracoccidioidomicose
Paracoccidioides
Imunologia
title_short Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice
title_full Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice
title_fullStr Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice
title_full_unstemmed Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice
title_sort Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice
author Meloni-Bruneri, L. H.
author_facet Meloni-Bruneri, L. H.
Campa, A.
Abdalla, D. S.
Calich, Vera Lucia G.
Lenzi, Henrique Leonel
Burger, Eva
author_role author
author2 Campa, A.
Abdalla, D. S.
Calich, Vera Lucia G.
Lenzi, Henrique Leonel
Burger, Eva
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Meloni-Bruneri, L. H.
Campa, A.
Abdalla, D. S.
Calich, Vera Lucia G.
Lenzi, Henrique Leonel
Burger, Eva
dc.subject.en.pt_BR.fl_str_mv Disease Susceptibility
Luminescent Measurements
Luminol
Neutrophils
Paracoccidioidomycosis
Paracoccidioides
topic Disease Susceptibility
Luminescent Measurements
Luminol
Neutrophils
Paracoccidioidomycosis
Paracoccidioides
Suscetibilidade a Doenças
Medições Luminescentes
Luminol
Neutrófilos
Paracoccidioidomicose
Paracoccidioides
Imunologia
dc.subject.decs.pt_BR.fl_str_mv Suscetibilidade a Doenças
Medições Luminescentes
Luminol
Neutrófilos
Paracoccidioidomicose
Paracoccidioides
Imunologia
description Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2012-04-13T18:50:41Z
dc.date.available.fl_str_mv 2012-04-13T18:50:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MELONI-BRUNERI, L. H. et al. Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice. Journal of Leukocyte Biology, v. 59, n. 4, p. 526-33, Apr. 1996.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3838
dc.identifier.issn.none.fl_str_mv 0741-5400
identifier_str_mv MELONI-BRUNERI, L. H. et al. Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice. Journal of Leukocyte Biology, v. 59, n. 4, p. 526-33, Apr. 1996.
0741-5400
url https://www.arca.fiocruz.br/handle/icict/3838
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for Leukocyte Biology
publisher.none.fl_str_mv Society for Leukocyte Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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