Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.

Franco, Priscila Silva; Gomes, Angelica de Oliveira; Barbosa, Bellisa de Freitas; Angeloni, Mariana Bodini; Silva, Neide Maria; Carvalho, Andréa Teixeira; Martins Filho, Olindo Assis; Silva, Daise Aparecida Oliveira; Mineo, José Roberto

Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.

Detalhes bibliográficos
Autor(a) principal:	Franco, Priscila Silva
Data de Publicação:	2011
Outros Autores:	Gomes, Angelica de Oliveira, Barbosa, Bellisa de Freitas, Angeloni, Mariana Bodini, Silva, Neide Maria, Carvalho, Andréa Teixeira, Martins Filho, Olindo Assis, Silva, Daise Aparecida Oliveira, Mineo, José Roberto
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/8683
Resumo:	Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil

Metadados do item

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repository_id_str	2135
spelling	Franco, Priscila SilvaGomes, Angelica de OliveiraBarbosa, Bellisa de FreitasAngeloni, Mariana BodiniSilva, Neide MariaCarvalho, Andréa TeixeiraMartins Filho, Olindo AssisSilva, Daise Aparecida OliveiraMineo, José Roberto2014-10-28T12:49:06Z2014-10-28T12:49:06Z2011FRANCO, Priscila Silva et at. Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection. Placenta, 32(11): 838-844, 2011.0143-400410.1016/j.placenta.2011.08.012https://www.arca.fiocruz.br/handle/icict/8683engElsevier Ltd.Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, BrazilUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, BrazilUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, BrazilUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, BrazilUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, BrazilUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, BrazilUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, BrazilToxoplasma gondii is an important pathogen which may cause fetal infection if primary infection. Our previous studies have used human choriocarcinoma trophoblastic cells (BeWo cell line) as experimental model of T. gondii infection involving placental microenvironment. This study aimed to examine the effects of azithromycin and spiramycin against T. gondiiinfection in BeWo cells. Cells were treated with different concentrations of the macrolide antibiotics and analyzed first for cell viability using thiazolyl blue tetrazole (MTT) assay. As cell viability was significantly decreased with drug concentrations higher than 400 μg/mL, the concentration range used in further experiments was from 50 to 400 μg/mL. The number of infected cells and intracellular replication of T. gondii decreased after treatment with each drug. The infection induced up-regulation of the macrophage migration inhibitory factor (MIF), which was also enhanced in infected cells after treatment with azithromycin, but not with spiramycin. Analysis of the cytokine profile showed increase TNF-α, IL-10 and IL-4 production, but decreased IFN-γ levels, were detected in infected cells and treated with each drug. In conclusion, treatment of human trophoblastic BeWo cells with with azithromycin or spiramycin is able to control the infection and replication of T. gondii. In addition, treatment with these macrolides, especially with azityromycin induces an anti-inflammatory response and high MIF production, which can be important for the establishment and maintenance of a viable pregnancy during T. gondii infection.AzithromycinSpiramycinBeWo cellsCytokinesToxoplasma gondiiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8683/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALAzithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.pdfAzithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.pdfapplication/pdf794274https://www.arca.fiocruz.br/bitstream/icict/8683/2/Azithromycin%20and%20spiramycin%20induce%20anti-inflammatory%20response%20in%20human%20trophoblastic%20%28BeWo%29%20cells%20infected%20by%20Toxoplasma%20gondii%20but%20are%20able%20to%20control%20infection.pdfe58f023f31657c60ddc41663faa9cec9MD52TEXTAzithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.pdf.txtAzithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.pdf.txtExtracted texttext/plain38391https://www.arca.fiocruz.br/bitstream/icict/8683/3/Azithromycin%20and%20spiramycin%20induce%20anti-inflammatory%20response%20in%20human%20trophoblastic%20%28BeWo%29%20cells%20infected%20by%20Toxoplasma%20gondii%20but%20are%20able%20to%20control%20infection.pdf.txte29001ccc8808f8dfc447fe86e5505f0MD53icict/86832019-06-19 10:12:34.076oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:12:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
title	Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
spellingShingle	Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection. Franco, Priscila Silva Azithromycin Spiramycin BeWo cells Cytokines Toxoplasma gondii
title_short	Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
title_full	Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
title_fullStr	Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
title_full_unstemmed	Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
title_sort	Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
author	Franco, Priscila Silva
author_facet	Franco, Priscila Silva Gomes, Angelica de Oliveira Barbosa, Bellisa de Freitas Angeloni, Mariana Bodini Silva, Neide Maria Carvalho, Andréa Teixeira Martins Filho, Olindo Assis Silva, Daise Aparecida Oliveira Mineo, José Roberto
author_role	author
author2	Gomes, Angelica de Oliveira Barbosa, Bellisa de Freitas Angeloni, Mariana Bodini Silva, Neide Maria Carvalho, Andréa Teixeira Martins Filho, Olindo Assis Silva, Daise Aparecida Oliveira Mineo, José Roberto
author2_role	author author author author author author author author
dc.contributor.author.fl_str_mv	Franco, Priscila Silva Gomes, Angelica de Oliveira Barbosa, Bellisa de Freitas Angeloni, Mariana Bodini Silva, Neide Maria Carvalho, Andréa Teixeira Martins Filho, Olindo Assis Silva, Daise Aparecida Oliveira Mineo, José Roberto
dc.subject.en.pt_BR.fl_str_mv	Azithromycin Spiramycin BeWo cells Cytokines Toxoplasma gondii
topic	Azithromycin Spiramycin BeWo cells Cytokines Toxoplasma gondii
description	Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
publishDate	2011
dc.date.issued.fl_str_mv	2011
dc.date.accessioned.fl_str_mv	2014-10-28T12:49:06Z
dc.date.available.fl_str_mv	2014-10-28T12:49:06Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	FRANCO, Priscila Silva et at. Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection. Placenta, 32(11): 838-844, 2011.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/8683
dc.identifier.issn.none.fl_str_mv	0143-4004 10.1016/j.placenta.2011.08.012
identifier_str_mv	FRANCO, Priscila Silva et at. Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection. Placenta, 32(11): 838-844, 2011. 0143-4004 10.1016/j.placenta.2011.08.012
url	https://www.arca.fiocruz.br/handle/icict/8683
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Elsevier Ltd.
publisher.none.fl_str_mv	Elsevier Ltd.
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.

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