Azithromycin is able to control Toxoplasma gondii infection in human villous explants

Detalhes bibliográficos
Autor(a) principal: Filice, Letícia de Souza Castro
Data de Publicação: 2014
Outros Autores: Barbosa, Bellisa de Freitas, Angeloni, Mariana Bodini, Silva, Neide Maria da, Gomes, Angelica de Oliveira, Alves, Celene M. O. S., Silva, Deise Aparecida de Oliveira, Martins Filho, Olindo Assis, Santos, Maria C., Mineo, Jose Roberto, Ferro, Eloisa Amalia Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9500
Resumo: Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunofisiologia da Reprodução. Uberlândia, MG, Brasil.
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spelling Filice, Letícia de Souza CastroBarbosa, Bellisa de FreitasAngeloni, Mariana BodiniSilva, Neide Maria daGomes, Angelica de OliveiraAlves, Celene M. O. S.Silva, Deise Aparecida de OliveiraMartins Filho, Olindo AssisSantos, Maria C.Mineo, Jose RobertoFerro, Eloisa Amalia Vieira2015-02-19T14:55:14Z2015-02-19T14:55:14Z2014FILICE, Letícia de Souza Castro et al. Azithromycin is able to control Toxoplasma gondii infection in human villous explants. Journal of Translational Medicine, v. 12, p. 132, 2014.1479-5876https://www.arca.fiocruz.br/handle/icict/9500engSpringer Co.Azithromycin is able to control Toxoplasma gondii infection in human villous explantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunofisiologia da Reprodução. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunofisiologia da Reprodução. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunofisiologia da Reprodução. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunofisiologia da Reprodução. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunofisiologia da Reprodução. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil.Universidade Federal de Uberlândia. Faculdade de Medicina. Departamento de Ginecologia e Obstetricia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciencias Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brasil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunofisiologia da Reprodução. Uberlândia, MG, Brasil.BACKGROUND: Although Toxoplasma gondii infection is normally asymptomatic, severe cases of toxoplasmosis may occur in immunosuppressed patients or congenitally infected newborns. When a fetal infection is established, the recommended treatment is a combination of pyrimethamine, sulfadiazine and folinic acid (PSA). The aim of the present study was to evaluate the efficacy of azithromycin to control T. gondii infection in human villous explants. METHODS: Cultures of third trimester human villous explants were infected with T. gondii and simultaneously treated with either PSA or azithromycin. Proliferation of T. gondii, as well as production of cytokines and hormones by chorionic villous explants, was analyzed. RESULTS: Treatment with either azithromycin or PSA was able to control T. gondii infection in villous explants. After azithromycin or PSA treatment, TNF-α, IL-17A or TGF-β1 levels secreted by infected villous explants did not present significant differences. However, PSA-treated villous explants had decreased levels of IL-10 and increased IL-12 levels, while treatment with azithromycin increased production of IL-6. Additionally, T. gondii-infected villous explants increased secretion of estradiol, progesterone and HCG+β, while treatments with azithromycin or PSA reduced secretion of these hormones concurrently with decrease of parasite load. CONCLUSIONS: In conclusion, these results suggest that azithromycin may be defined as an effective alternative drug to control T. gondii infection at the fetal-maternal interfaceAzithromycinHuman placental villiTrophoblastCytokinesSex hormonesToxoplasma gondiiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9500/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_084.pdf2014_084.pdfapplication/pdf1442525https://www.arca.fiocruz.br/bitstream/icict/9500/2/2014_084.pdf0ea3f08436712815ef93363ee3beae9eMD52TEXT2014_084.pdf.txt2014_084.pdf.txtExtracted texttext/plain49677https://www.arca.fiocruz.br/bitstream/icict/9500/3/2014_084.pdf.txt56fa6eb50766a7d2ea8b72d51a1a95c2MD53icict/95002019-06-19 10:07:35.332oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:07:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Azithromycin is able to control Toxoplasma gondii infection in human villous explants
title Azithromycin is able to control Toxoplasma gondii infection in human villous explants
spellingShingle Azithromycin is able to control Toxoplasma gondii infection in human villous explants
Filice, Letícia de Souza Castro
Azithromycin
Human placental villi
Trophoblast
Cytokines
Sex hormones
Toxoplasma gondii
title_short Azithromycin is able to control Toxoplasma gondii infection in human villous explants
title_full Azithromycin is able to control Toxoplasma gondii infection in human villous explants
title_fullStr Azithromycin is able to control Toxoplasma gondii infection in human villous explants
title_full_unstemmed Azithromycin is able to control Toxoplasma gondii infection in human villous explants
title_sort Azithromycin is able to control Toxoplasma gondii infection in human villous explants
author Filice, Letícia de Souza Castro
author_facet Filice, Letícia de Souza Castro
Barbosa, Bellisa de Freitas
Angeloni, Mariana Bodini
Silva, Neide Maria da
Gomes, Angelica de Oliveira
Alves, Celene M. O. S.
Silva, Deise Aparecida de Oliveira
Martins Filho, Olindo Assis
Santos, Maria C.
Mineo, Jose Roberto
Ferro, Eloisa Amalia Vieira
author_role author
author2 Barbosa, Bellisa de Freitas
Angeloni, Mariana Bodini
Silva, Neide Maria da
Gomes, Angelica de Oliveira
Alves, Celene M. O. S.
Silva, Deise Aparecida de Oliveira
Martins Filho, Olindo Assis
Santos, Maria C.
Mineo, Jose Roberto
Ferro, Eloisa Amalia Vieira
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Filice, Letícia de Souza Castro
Barbosa, Bellisa de Freitas
Angeloni, Mariana Bodini
Silva, Neide Maria da
Gomes, Angelica de Oliveira
Alves, Celene M. O. S.
Silva, Deise Aparecida de Oliveira
Martins Filho, Olindo Assis
Santos, Maria C.
Mineo, Jose Roberto
Ferro, Eloisa Amalia Vieira
dc.subject.en.pt_BR.fl_str_mv Azithromycin
Human placental villi
Trophoblast
Cytokines
Sex hormones
Toxoplasma gondii
topic Azithromycin
Human placental villi
Trophoblast
Cytokines
Sex hormones
Toxoplasma gondii
description Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunofisiologia da Reprodução. Uberlândia, MG, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-02-19T14:55:14Z
dc.date.available.fl_str_mv 2015-02-19T14:55:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv FILICE, Letícia de Souza Castro et al. Azithromycin is able to control Toxoplasma gondii infection in human villous explants. Journal of Translational Medicine, v. 12, p. 132, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9500
dc.identifier.issn.none.fl_str_mv 1479-5876
identifier_str_mv FILICE, Letícia de Souza Castro et al. Azithromycin is able to control Toxoplasma gondii infection in human villous explants. Journal of Translational Medicine, v. 12, p. 132, 2014.
1479-5876
url https://www.arca.fiocruz.br/handle/icict/9500
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