Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51153 |
Resumo: | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia e Biologia Molecular. Salvador, BA, Brazil. |
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Sampaio, Julia RamosSoares, Rodrigo Pedro PintoBarral, Thiago DoriaPassos, Gabriela PorfirioFonseca, Maisa SantosMeyer, RobertoBarrouin-Melo, Stella MariaPortela, Ricardo Wagner2022-02-11T13:22:13Z2022-02-11T13:22:13Z2021SAMPAIO, Julia Ramos et al. Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs. Front Vet Sci, v. 8, 581148, 2021. doi: 10.3389/fvets.2021.581148. eCollection 2021.2297-1769https://www.arca.fiocruz.br/handle/icict/51153porFrontiers Media S.ALeishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia e Biologia Molecular. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia e Biologia Molecular. Salvador, BA, Brazil.Departamento de Anatomia. Patologia e Clínicas. Escola de Medicina Veterinária e Zootecnia. Universidade Federal da Bahia. Salvador, BA, Brazil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia e Biologia Molecular. Salvador, BA, Brazil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia e Biologia Molecular. Salvador, BA, Brazil.Departamento de Anatomia. Patologia e Clínicas. Escola de Medicina Veterinária e Zootecnia. Universidade Federal da Bahia. Salvador, BA, Brazil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia e Biologia Molecular. Salvador, BA, Brazil.Lipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of Leishmania infantum antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which allows their use in point-of-care testing without special storage conditions. During the glycoconjugate extraction, a glycoinositolphospholipid (GIPL)-enriched fraction is obtained in similar quantities as LPG. Since GIPLs can be extracted from the same parasite pellet as LPGs, this work aimed to evaluate the immune recognition of GIPLs by Leishmania infantum-infected dogs and its use for canine leishmaniasis (CanL) immunodiagnosis. Like LPG, GIPLs were recognized by sera from L. infantum-infected dogs, but with less sensitivity (83.8%). However, 80% (16/20) of subclinically infected dogs were detected as positive in the assay. Different from LPG, the GIPL-based assay achieved a lower specificity (73.7%) and cross-reactions occurred with T. cruzi and L. braziliensis-infected dogs. Although GIPLs exhibited a similar performance to LPG for subclinically L. infantum-infected dogs, the occurrence of cross-reactivities with other protozoa and a lower sensitivity hinders its use for an immunodiagnostic test. In places where those diseases do not co-exist such as in the Mediterranean region, its use for subclinically dogs could be an alternative.ELISALeishmania infantumdogsglycoconjugatesimmunodiagnosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51153/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALLeishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs.pdfLeishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs.pdfapplication/pdf3005830https://www.arca.fiocruz.br/bitstream/icict/51153/2/Leishmania%20infantum-Derived%20Glycoinositolphospholipids%20in%20the%20Immunodiagnosis%20of%20Subclinically%20Infected%20Dogs.pdf5fc87e7aa1afc0a5ee14f25884460fadMD52icict/511532022-02-11 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| dc.title.pt_BR.fl_str_mv |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
| title |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
| spellingShingle |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs Sampaio, Julia Ramos ELISA Leishmania infantum dogs glycoconjugates immunodiagnosis |
| title_short |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
| title_full |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
| title_fullStr |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
| title_full_unstemmed |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
| title_sort |
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs |
| author |
Sampaio, Julia Ramos |
| author_facet |
Sampaio, Julia Ramos Soares, Rodrigo Pedro Pinto Barral, Thiago Doria Passos, Gabriela Porfirio Fonseca, Maisa Santos Meyer, Roberto Barrouin-Melo, Stella Maria Portela, Ricardo Wagner |
| author_role |
author |
| author2 |
Soares, Rodrigo Pedro Pinto Barral, Thiago Doria Passos, Gabriela Porfirio Fonseca, Maisa Santos Meyer, Roberto Barrouin-Melo, Stella Maria Portela, Ricardo Wagner |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sampaio, Julia Ramos Soares, Rodrigo Pedro Pinto Barral, Thiago Doria Passos, Gabriela Porfirio Fonseca, Maisa Santos Meyer, Roberto Barrouin-Melo, Stella Maria Portela, Ricardo Wagner |
| dc.subject.en.pt_BR.fl_str_mv |
ELISA Leishmania infantum dogs glycoconjugates immunodiagnosis |
| topic |
ELISA Leishmania infantum dogs glycoconjugates immunodiagnosis |
| description |
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia e Biologia Molecular. Salvador, BA, Brazil. |
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2021 |
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2021 |
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2022-02-11T13:22:13Z |
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2022-02-11T13:22:13Z |
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SAMPAIO, Julia Ramos et al. Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs. Front Vet Sci, v. 8, 581148, 2021. doi: 10.3389/fvets.2021.581148. eCollection 2021. |
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https://www.arca.fiocruz.br/handle/icict/51153 |
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2297-1769 |
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SAMPAIO, Julia Ramos et al. Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs. Front Vet Sci, v. 8, 581148, 2021. doi: 10.3389/fvets.2021.581148. eCollection 2021. 2297-1769 |
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Frontiers Media S.A |
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