Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42271 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. |
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Veloso, Laura BarralCardoso, Flávia de OliveiraCharret, Karen dos SantosSilva, Matheus Pereira de SáCôrtes, Luzia Monteiro de CastroCalabrese, Kátia da SilvaSilva, Franklin Souza daSousa, Joel Fontes deRocha, Marília FonsecaAlves, Carlos Roberto2020-07-15T21:04:00Z2020-07-15T21:04:00Z2020VELOSO, Laura Barral et al. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International, v. 2020, Article ID 2615787, 8p, 2020.2314-6133https://www.arca.fiocruz.br/handle/icict/422712314-6141engHindawiLeishmania (Leishmania) infantumDetecção de RNAMetaloproteasesProteases de cisteínaLeishmania (Leishmania) infantumInfecçãoPeles de cãesLeishmania (Leishmania) infantumMetalloproteasesCysteine ProteasesDetectionInfected DogsDetection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Prefeitura Municipal de Montes Claros. Centro de Controle de Zoonoses. Montes Claros, MG, Brasil.Prefeitura Municipal de Montes Claros. Centro de Controle de Zoonoses. Montes Claros, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Leishmania spp. proteases have been proposed as virulence factors contributing to adaptive success these parasites to the mammalian hosts. Since these enzymes are poorly studied in naturally infected dogs, this work aims to show the differences in metalloprotease and cysteine proteases gene expression in ear edge skin of dogs naturally infected by Leishmania (Leishmania) infantum. A cohort of dogs (n = 20) naturally infected by L. (L.) infantum was clinically classified as asymptomatic, oligosymptomatic, and polysymptomatic and the parasite load range estimated. The analysis of proteases expression by RT-PCR in the ear edge skin was also assessed, suggesting more transcripts of proteases in cDNA samples from polysymptomatic dogs than oligosymptomatic and asymptomatic ones. Metalloprotease RT-PCR assays yielded products (202 bp) in all assessed cDNA dog samples. In contrast, cysteine proteases transcripts (227 bp) had shown to be better detected in cDNA samples of polysymptomatic dogs, compared with cDNA samples from asymptomatic and oligosymptomatic dogs. Predictive in silico assays suggested that secondary structures of metalloproteasee mRNAs can be more stable than cysteine proteases at the skin temperature of dogs. Evidence is presented that during natural infection of dogs by L. (L.) infantum, this parasite produces transcripts of metalloprotease and cysteine protease RNA in the skin from asymptomatic, oligosymptomatic, and polysymptomatic dogs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42271/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLauraBVeloso_KarenCharret_etal_IOC_2020.pdfLauraBVeloso_KarenCharret_etal_IOC_2020.pdfapplication/pdf665809https://www.arca.fiocruz.br/bitstream/icict/42271/2/LauraBVeloso_KarenCharret_etal_IOC_2020.pdf52c4ab7519f9734309eadb74e4ffbaa6MD52TEXTLauraBVeloso_KarenCharret_etal_IOC_2020.pdf.txtLauraBVeloso_KarenCharret_etal_IOC_2020.pdf.txtExtracted texttext/plain41303https://www.arca.fiocruz.br/bitstream/icict/42271/3/LauraBVeloso_KarenCharret_etal_IOC_2020.pdf.txtc4f01b04aa1ae8dcdb7677bccfcbaf22MD53icict/422712022-06-24 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dc.title.pt_BR.fl_str_mv |
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs |
title |
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs |
spellingShingle |
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs Veloso, Laura Barral Leishmania (Leishmania) infantum Detecção de RNA Metaloproteases Proteases de cisteína Leishmania (Leishmania) infantum Infecção Peles de cães Leishmania (Leishmania) infantum Metalloproteases Cysteine Proteases Detection Infected Dogs |
title_short |
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs |
title_full |
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs |
title_fullStr |
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs |
title_full_unstemmed |
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs |
title_sort |
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs |
author |
Veloso, Laura Barral |
author_facet |
Veloso, Laura Barral Cardoso, Flávia de Oliveira Charret, Karen dos Santos Silva, Matheus Pereira de Sá Côrtes, Luzia Monteiro de Castro Calabrese, Kátia da Silva Silva, Franklin Souza da Sousa, Joel Fontes de Rocha, Marília Fonseca Alves, Carlos Roberto |
author_role |
author |
author2 |
Cardoso, Flávia de Oliveira Charret, Karen dos Santos Silva, Matheus Pereira de Sá Côrtes, Luzia Monteiro de Castro Calabrese, Kátia da Silva Silva, Franklin Souza da Sousa, Joel Fontes de Rocha, Marília Fonseca Alves, Carlos Roberto |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Veloso, Laura Barral Cardoso, Flávia de Oliveira Charret, Karen dos Santos Silva, Matheus Pereira de Sá Côrtes, Luzia Monteiro de Castro Calabrese, Kátia da Silva Silva, Franklin Souza da Sousa, Joel Fontes de Rocha, Marília Fonseca Alves, Carlos Roberto |
dc.subject.other.pt_BR.fl_str_mv |
Leishmania (Leishmania) infantum Detecção de RNA Metaloproteases Proteases de cisteína Leishmania (Leishmania) infantum Infecção Peles de cães |
topic |
Leishmania (Leishmania) infantum Detecção de RNA Metaloproteases Proteases de cisteína Leishmania (Leishmania) infantum Infecção Peles de cães Leishmania (Leishmania) infantum Metalloproteases Cysteine Proteases Detection Infected Dogs |
dc.subject.en.pt_BR.fl_str_mv |
Leishmania (Leishmania) infantum Metalloproteases Cysteine Proteases Detection Infected Dogs |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-07-15T21:04:00Z |
dc.date.available.fl_str_mv |
2020-07-15T21:04:00Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VELOSO, Laura Barral et al. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International, v. 2020, Article ID 2615787, 8p, 2020. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/42271 |
dc.identifier.issn.pt_BR.fl_str_mv |
2314-6133 |
dc.identifier.eissn.none.fl_str_mv |
2314-6141 |
identifier_str_mv |
VELOSO, Laura Barral et al. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International, v. 2020, Article ID 2615787, 8p, 2020. 2314-6133 2314-6141 |
url |
https://www.arca.fiocruz.br/handle/icict/42271 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Hindawi |
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Hindawi |
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