Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species

Detalhes bibliográficos
Autor(a) principal: Oddone, Rolando
Data de Publicação: 2009
Outros Autores: Schweynoch, Carola, Schönian, Gabriele, Sousa, Cintia dos Santos de, Cupolillo, Elisa, Espinosa, Diego, Arevalo, Jorge, Noyes, Harry, Mauricio, Isabel, Kuhls, Katrin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28331
Resumo: Universita¨tsmedizin Berlin. Institut fu¨r Mikrobiologie und Hygiene. Berlin, Germany / Universidad Nacional de Asuncion. Instituto de Investigacionesn Ciencias de la Salud. Asunción, Paraguay.
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spelling Oddone, RolandoSchweynoch, CarolaSchönian, GabrieleSousa, Cintia dos Santos deCupolillo, ElisaEspinosa, DiegoArevalo, JorgeNoyes, HarryMauricio, IsabelKuhls, Katrin2018-08-23T15:16:16Z2018-08-23T15:16:16Z2009ODDONE, Rolando; et al. Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of Leishmania (Viannia) Species. Journal of Clinical Microbiology, v.47, n.9, p.2818-2825, Sept. 2009.0095-1137https://www.arca.fiocruz.br/handle/icict/2833110.1128/JCM.00645-091098-660XengAmerican Society for MicrobiologyEspécies de Leishmania Vianniamicrossatélites MultilocusCepas discriminantesLeishmania (Viannia) speciesMultilocus MicrosatelliteDiscriminating strainsDevelopment of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversita¨tsmedizin Berlin. Institut fu¨r Mikrobiologie und Hygiene. Berlin, Germany / Universidad Nacional de Asuncion. Instituto de Investigacionesn Ciencias de la Salud. Asunción, Paraguay.Universita¨tsmedizin Berlin. Institut fu¨r Mikrobiologie und Hygiene. Berlin, Germany.Universita¨tsmedizin Berlin. Institut fu¨r Mikrobiologie und Hygiene. Berlin, Germany.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ. Brasil.Instituto de Medicina Tropical Alexander von Humboldt. Lima, Peru.Instituto de Medicina Tropical Alexander von Humboldt. Lima, Peru.University of Liverpool. Biosciences Department. Liverpool, United Kingdom.London School of Hygiene and Tropical Medicine. London, United Kingdom.Universita¨tsmedizin Berlin. Institut fu¨r Mikrobiologie und Hygiene. Berlin, Germany.A multilocus microsatellite typing (MLMT) approach based on the analysis of 15 independent loci has been developed for the discrimination of strains belonging to different Viannia species. Thirteen microsatellite loci were isolated de novo from microsatellite-enriched libraries for both Leishmania braziliensis and L. guyanensis. Two previously identified markers, AC01 and AC16, were modified and added to our marker set. Markers were designed to contain simple dinucleotide repeats flanked by the minimal possible number of nucleotides in order to allow variations in repeat numbers to be scored as size variations of the PCR products. The 15 markers in total were amplified for almost all of the strains of Viannia tested; one marker did not amplify from the two L. peruviana strains included in the study. When 30 strains of L. braziliensis, 21 strains of L. guyanensis, and 2 strains of L. peruviana were tested for polymorphisms, all strains except two strains of L. guyanensis had individual MLMT types. Distance-based analysis identified three main clusters. All strains except one strain of L. guyanensis grouped together. Two clusters consisted of strains of L. braziliensis according to their geographical origins. The two strains of L. peruviana grouped together with strains of L. braziliensis from Peru and the adjacent Brazilian state of Acre. MLMT has proven capable of individualizing strains even from the same areas of endemicity and of detecting genetic structures at different levels. MLMT is thus applicable for epidemiological and population genetic studies of strains within the subgenus Viannia.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28331/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrolando_odonne_etal_IOC_2009.pdfrolando_odonne_etal_IOC_2009.pdfapplication/pdf599935https://www.arca.fiocruz.br/bitstream/icict/28331/2/rolando_odonne_etal_IOC_2009.pdf8a6b81d3c04df908c66d03d3ea2d6dffMD52TEXTrolando_odonne_etal_IOC_2009.pdf.txtrolando_odonne_etal_IOC_2009.pdf.txtExtracted texttext/plain46081https://www.arca.fiocruz.br/bitstream/icict/28331/3/rolando_odonne_etal_IOC_2009.pdf.txt5f03da69829cdcb3ad87a6602d179ef5MD53icict/283312018-08-24 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dc.title.pt_BR.fl_str_mv Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species
title Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species
spellingShingle Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species
Oddone, Rolando
Espécies de Leishmania Viannia
microssatélites Multilocus
Cepas discriminantes
Leishmania (Viannia) species
Multilocus Microsatellite
Discriminating strains
title_short Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species
title_full Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species
title_fullStr Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species
title_full_unstemmed Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species
title_sort Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species
author Oddone, Rolando
author_facet Oddone, Rolando
Schweynoch, Carola
Schönian, Gabriele
Sousa, Cintia dos Santos de
Cupolillo, Elisa
Espinosa, Diego
Arevalo, Jorge
Noyes, Harry
Mauricio, Isabel
Kuhls, Katrin
author_role author
author2 Schweynoch, Carola
Schönian, Gabriele
Sousa, Cintia dos Santos de
Cupolillo, Elisa
Espinosa, Diego
Arevalo, Jorge
Noyes, Harry
Mauricio, Isabel
Kuhls, Katrin
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oddone, Rolando
Schweynoch, Carola
Schönian, Gabriele
Sousa, Cintia dos Santos de
Cupolillo, Elisa
Espinosa, Diego
Arevalo, Jorge
Noyes, Harry
Mauricio, Isabel
Kuhls, Katrin
dc.subject.other.pt_BR.fl_str_mv Espécies de Leishmania Viannia
microssatélites Multilocus
Cepas discriminantes
topic Espécies de Leishmania Viannia
microssatélites Multilocus
Cepas discriminantes
Leishmania (Viannia) species
Multilocus Microsatellite
Discriminating strains
dc.subject.en.pt_BR.fl_str_mv Leishmania (Viannia) species
Multilocus Microsatellite
Discriminating strains
description Universita¨tsmedizin Berlin. Institut fu¨r Mikrobiologie und Hygiene. Berlin, Germany / Universidad Nacional de Asuncion. Instituto de Investigacionesn Ciencias de la Salud. Asunción, Paraguay.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-08-23T15:16:16Z
dc.date.available.fl_str_mv 2018-08-23T15:16:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ODDONE, Rolando; et al. Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of Leishmania (Viannia) Species. Journal of Clinical Microbiology, v.47, n.9, p.2818-2825, Sept. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28331
dc.identifier.issn.pt_BR.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.00645-09
dc.identifier.eissn.none.fl_str_mv 1098-660X
identifier_str_mv ODDONE, Rolando; et al. Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of Leishmania (Viannia) Species. Journal of Clinical Microbiology, v.47, n.9, p.2818-2825, Sept. 2009.
0095-1137
10.1128/JCM.00645-09
1098-660X
url https://www.arca.fiocruz.br/handle/icict/28331
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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