The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion

Detalhes bibliográficos
Autor(a) principal: Freitas, Carla
Data de Publicação: 2012
Outros Autores: Assis, Maria Cristina, Saliba, Alessandra Mattos, Morandi, Veronica Maria, Figueiredo, Camila Castro, Pereira, Mirian, Plotkowski, Maria Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18796
Resumo: Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil.
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spelling Freitas, CarlaAssis, Maria CristinaSaliba, Alessandra MattosMorandi, Veronica MariaFigueiredo, Camila CastroPereira, MirianPlotkowski, Maria Cristina2017-05-09T15:58:14Z2017-05-09T15:58:14Z2012FREITAS, Carla; et al. The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion. Mem Inst Oswaldo Cruz, v.107, n.6, p.728-734, Sept. 2012.0074-0276https://www.arca.fiocruz.br/handle/icict/1879610.1590/S0074-027620120006000041678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzPseudomonas aeruginosaFator de von WillebrandAtividade protrombóticaMicropartículasPseudomonas aeruginosavon Willebrand factorprothrombotic activitymicroparticlesThe infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil.Centro Universitário Estadual da Zona Oeste. Departamento de Ciências Biológicas e da Saúde. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-Estrutura e Biologia Celular. Rio de Janeiro, RJ. Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil.An increased plasma concentration of von Willebrand factor (vWF) is detected in individuals with many infectious diseases and is accepted as a marker of endothelium activation and prothrombotic condition. To determine whether ExoU, a Pseudomonas aeruginosa cytotoxin with proinflammatory activity, enhances the release of vWF, microvascular endothelial cells were infected with the ExoU-producing PA103 P. aeruginosa strain or an exoU-deficient mutant. Significantly increased vWF concentrations were detected in conditioned medium and subendothelial extracellular matrix from cultures infected with the wild-type bacteria, as determined by enzyme-linked immunoassays. PA103-infected cells also released higher concentrations of procoagulant microparticles containing increased amounts of membrane-associated vWF, as determined by flow cytometric analyses of cell culture supernatants. Both flow cytometry and confocal microscopy showed that increased amounts of vWF were associated with cytoplasmic membranes from cells infected with the ExoU-producing bacteria. PA103-infected cultures exposed to platelet suspensions exhibited increased percentages of cells with platelet adhesion. Because no modulation of the vWF mRNA levels was detected by reverse transcription-polymerase chain reaction assays in PA103-infected cells, ExoU is likely to have induced the release of vWF from cytoplasmic stores rather than vWF gene transcription. Such release is likely to modify the thromboresistance of microvascular endothelial cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18796/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmiriam_pereira_etal_IOC_2012.pdfmiriam_pereira_etal_IOC_2012.pdfapplication/pdf1172987https://www.arca.fiocruz.br/bitstream/icict/18796/2/miriam_pereira_etal_IOC_2012.pdf77b56820c786cb755c383e2832744152MD52TEXTmiriam_pereira_etal_IOC_2012.pdf.txtmiriam_pereira_etal_IOC_2012.pdf.txtExtracted texttext/plain35183https://www.arca.fiocruz.br/bitstream/icict/18796/3/miriam_pereira_etal_IOC_2012.pdf.txt9972de2c95433a9dc177ba39dc59c960MD53icict/187962023-09-05 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dc.title.pt_BR.fl_str_mv The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
title The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
spellingShingle The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
Freitas, Carla
Pseudomonas aeruginosa
Fator de von Willebrand
Atividade protrombótica
Micropartículas
Pseudomonas aeruginosa
von Willebrand factor
prothrombotic activity
microparticles
title_short The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
title_full The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
title_fullStr The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
title_full_unstemmed The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
title_sort The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion
author Freitas, Carla
author_facet Freitas, Carla
Assis, Maria Cristina
Saliba, Alessandra Mattos
Morandi, Veronica Maria
Figueiredo, Camila Castro
Pereira, Mirian
Plotkowski, Maria Cristina
author_role author
author2 Assis, Maria Cristina
Saliba, Alessandra Mattos
Morandi, Veronica Maria
Figueiredo, Camila Castro
Pereira, Mirian
Plotkowski, Maria Cristina
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Freitas, Carla
Assis, Maria Cristina
Saliba, Alessandra Mattos
Morandi, Veronica Maria
Figueiredo, Camila Castro
Pereira, Mirian
Plotkowski, Maria Cristina
dc.subject.other.pt_BR.fl_str_mv Pseudomonas aeruginosa
Fator de von Willebrand
Atividade protrombótica
Micropartículas
topic Pseudomonas aeruginosa
Fator de von Willebrand
Atividade protrombótica
Micropartículas
Pseudomonas aeruginosa
von Willebrand factor
prothrombotic activity
microparticles
dc.subject.en.pt_BR.fl_str_mv Pseudomonas aeruginosa
von Willebrand factor
prothrombotic activity
microparticles
description Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-05-09T15:58:14Z
dc.date.available.fl_str_mv 2017-05-09T15:58:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FREITAS, Carla; et al. The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion. Mem Inst Oswaldo Cruz, v.107, n.6, p.728-734, Sept. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18796
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762012000600004
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FREITAS, Carla; et al. The infection of microvascular endothelial cells with ExoU-producing Pseudomonas aeruginosa triggers the release of von Willebrand factor and platelet adhesion. Mem Inst Oswaldo Cruz, v.107, n.6, p.728-734, Sept. 2012.
0074-0276
10.1590/S0074-02762012000600004
1678-8060
url https://www.arca.fiocruz.br/handle/icict/18796
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/18796/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/18796/2/miriam_pereira_etal_IOC_2012.pdf
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