Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23162 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil. |
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Vieira, Debora Ferreira BarretoJácome, Fernanda CunhaSilva, Marcos Alexandre Nunes daCaldas, Gabriela CardosoFilippis, Ana Maria Bispo deSequeira, Patrícia Carvalho deSouza, Elen Mello deAndrade, Audrien AlvesManso, Pedro Paulo de AbreuTrindade, Gisela FreitasLima, Sheila Maria BarbosaBarth, Ortrud Monika2017-11-14T13:12:48Z2017-11-14T13:12:48Z2017VIEIRA, Debora Ferreira Barreto; et al. Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus. Plos One, v.12, n.9, e0184397, 18p, Sept. 2107.1932-6203https://www.arca.fiocruz.br/handle/icict/2316210.1371/journal.pone.0184397engPublic Library of ScienceZika VirusBrasilCélulas VeroPesquisaZika virusBrazilVero cellsC6/36 cellsstructural investigationStructural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil .Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR. The infection was observed in both cell lineages, and ZIKV particles were observed inside lysosomes, the rough endoplasmic reticulum and viroplasm-like structures. The susceptibility of C6/36 and Vero cells to ZIKV infection was demonstrated. Moreover, this study showed that part of the replicative cycle may occur within viroplasm-like structures, which has not been previously demonstrated in other flaviviruses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83142https://www.arca.fiocruz.br/bitstream/icict/23162/1/license.txt37a74d92f1f1fd437ab11671160e0230MD51ORIGINALve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017 .pdfve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017 .pdfapplication/pdf32395268https://www.arca.fiocruz.br/bitstream/icict/23162/2/ve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017%20.pdfa29aeece74c9bb979c75face28995a5cMD52TEXTve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017 .pdf.txtve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017 .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus |
title |
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus |
spellingShingle |
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus Vieira, Debora Ferreira Barreto Zika Virus Brasil Células Vero Pesquisa Zika virus Brazil Vero cells C6/36 cells structural investigation |
title_short |
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus |
title_full |
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus |
title_fullStr |
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus |
title_full_unstemmed |
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus |
title_sort |
Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus |
author |
Vieira, Debora Ferreira Barreto |
author_facet |
Vieira, Debora Ferreira Barreto Jácome, Fernanda Cunha Silva, Marcos Alexandre Nunes da Caldas, Gabriela Cardoso Filippis, Ana Maria Bispo de Sequeira, Patrícia Carvalho de Souza, Elen Mello de Andrade, Audrien Alves Manso, Pedro Paulo de Abreu Trindade, Gisela Freitas Lima, Sheila Maria Barbosa Barth, Ortrud Monika |
author_role |
author |
author2 |
Jácome, Fernanda Cunha Silva, Marcos Alexandre Nunes da Caldas, Gabriela Cardoso Filippis, Ana Maria Bispo de Sequeira, Patrícia Carvalho de Souza, Elen Mello de Andrade, Audrien Alves Manso, Pedro Paulo de Abreu Trindade, Gisela Freitas Lima, Sheila Maria Barbosa Barth, Ortrud Monika |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Debora Ferreira Barreto Jácome, Fernanda Cunha Silva, Marcos Alexandre Nunes da Caldas, Gabriela Cardoso Filippis, Ana Maria Bispo de Sequeira, Patrícia Carvalho de Souza, Elen Mello de Andrade, Audrien Alves Manso, Pedro Paulo de Abreu Trindade, Gisela Freitas Lima, Sheila Maria Barbosa Barth, Ortrud Monika |
dc.subject.other.pt_BR.fl_str_mv |
Zika Virus Brasil Células Vero Pesquisa |
topic |
Zika Virus Brasil Células Vero Pesquisa Zika virus Brazil Vero cells C6/36 cells structural investigation |
dc.subject.en.pt_BR.fl_str_mv |
Zika virus Brazil Vero cells C6/36 cells structural investigation |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-11-14T13:12:48Z |
dc.date.available.fl_str_mv |
2017-11-14T13:12:48Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VIEIRA, Debora Ferreira Barreto; et al. Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus. Plos One, v.12, n.9, e0184397, 18p, Sept. 2107. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/23162 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0184397 |
identifier_str_mv |
VIEIRA, Debora Ferreira Barreto; et al. Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus. Plos One, v.12, n.9, e0184397, 18p, Sept. 2107. 1932-6203 10.1371/journal.pone.0184397 |
url |
https://www.arca.fiocruz.br/handle/icict/23162 |
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eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
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Public Library of Science |
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